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MABT913 Anti-Cytokeratin 19 Antibody, clone TROMA-3

Anti-Cytokeratin 19, clone TROMA-3, Cat. No. MABT913, is a rat monoclonal antibody that detects Keratin, type I cytoskeletal 19 and is tested for use in Electron Microscopy, Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

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MABT913
100 μg  
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Overview

Replacement Information
Description
Catalogue NumberMABT913
DescriptionAnti-Cytokeratin 19 Antibody, clone TROMA-3
Alternate Names
  • Keratin
  • type I cytoskeletal 19
  • CK-19
  • Keratin-19
  • K19
Background InformationKeratin, type I cytoskeletal 19 (UniProt: P19001; also known as Cytokeratin-19, CK-19, Keratin-19, K19) is encoded by the Krt19 (also known as Krt1-19) gene (Gene ID: 16669) in murine species. CK-19 belongs to the family of intermediate filament (IF) family, but differs from other IF proteins in lacking the C-terminal tail domain. About 20 different cytokeratin proteins have been reported in higher animals in varying combinations during various stages of development. CK-19 is normally expressed in the lining of the gastroenteropancreatic and hepatobiliary tracts. It is expressed throughout embryonic development with highest levels observed at 8.5 days post coitum. Its expression is shown to decrease by day 11.5 and then is increased again by 17.5 days post coitum. CK-19 is shown to be involved in the organization of myofibrils and together with keratin 8, it helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. CK-19 has been shown to be an independent prognostic factor for pancreatic neuroendocrine tumors, especially the insulin-negative tumors. TROMA 3 has been found to stain trophoblastoma TDM.1 cells, a subpopulation of the retinoic acid.-induced F9 and PCC3/ND1 cells, and in 5-azacytidine-treated 1246 cells.
References
Product Information
FormatPurified
PresentationPurified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Cytokeratin 19, clone TROMA-3, Cat. No. MABT913, is a rat monoclonal antibody that detects Keratin, type I cytoskeletal 19 and is tested for use in Electron Microscopy, Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
Key Applications
  • Western Blotting
  • Electron Microscopy
  • Immunoprecipitation
  • Immunohistochemistry (Paraffin)
  • Immunofluorescence
Application NotesImmunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Cytokeratin 19 in mouse lung tissue.

Western Blotting Analysis: A representative lot detected Cytokeratin 19 in Western Blotting applications (Boller, K., et. al. (1987). Eur J Cell Biol. 43(3):459-68).

Electron Microscopy Analysis: A representative lot detected Cytokeratin 19 in Electron Microscopy applications (Boller, K., et. al. (1987). Eur J Cell Biol. 43(3):459-68).

Immunofluorescence Analysis: A representative lot detected Cytokeratin 19 in Immunofluorescence applications (Boller, K., et. al. (1987). Eur J Cell Biol. 43(3):459-68).

Immunohistochemistry Analysis: A representative lot detected Cytokeratin 19 in Immunohistochemistry applications (Takase, H.M., et. al. (2013). Genes Dev. 27(2):169-81).

Immunoprecipitation Analysis: A representative lot detected Cytokeratin 19 in Immunoprecipitation applications (Ichinose, Y., et. al. (1990). Biochem Biophys Res Commun. 167(2):644-7).
Biological Information
ImmunogenPurified intermediate filaments from DM1 (Trophoblatoma) cell line.
CloneTROMA-3
ConcentrationPlease refer to lot specific datasheet.
HostRat
SpecificityClone TROMA-3 detects cytokeratin 19 in human and murine cells.
IsotypeIgG2aκ
Species Reactivity
  • Mouse
  • Human
Species Reactivity NoteMouse, Human.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Krt19
  • Krt1-19
Purification MethodProtein G purified
UniProt Number
Molecular Weight~44 kDa observed; 44.54 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in MCF-7 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Cytokeratin 19 in 10 µg of MCF-7 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABT913 04054839315985

Documentation

Anti-Cytokeratin 19 Antibody, clone TROMA-3 MSDS

Title

Safety Data Sheet (SDS) 

Anti-Cytokeratin 19 Antibody, clone TROMA-3 Certificates of Analysis

TitleLot Number
Anti-Cytokeratin 19, clone TROMA-3 3072292
Anti-Cytokeratin 19, clone TROMA-3 - 3261272 3261272
Anti-Cytokeratin 19, clone TROMA-3 - 3379915 3379915
Anti-Cytokeratin 19, clone TROMA-3 - 3698072 3698072
Anti-Cytokeratin 19, clone TROMA-3 - 3931681 3931681
Anti-Cytokeratin 19, clone TROMA-3 - 4017746 4017746
Anti-Cytokeratin 19, clone TROMA-3 - 4052390 4052390
Anti-Cytokeratin 19, clone TROMA-3 - 4092651 4092651
Anti-Cytokeratin 19, clone TROMA-3 - 4144886 4144886
Anti-Cytokeratin 19, clone TROMA-3 - 4219968 4219968
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