Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

07-1708 Anti-FOXH1 Antibody

Anti-FOXH1 Antibody is a Rabbit Polyclonal Antibody for detection of FOXH1 also known as Forkhead activin signal transducer, TGF-beta/activin signal transduce & has been validated in ICC.

Anti-FOXH1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Transcription Factors Gene Symbol: FAST-1, FAST1, FAST2, Fast-1, Fast-2, hFAST-1 UniProt Number: O75593
View Products on Sigmaaldrich.com
07-1708
100 µg  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, HICCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number07-1708
DescriptionAnti-FOXH1 Antibody
Alternate Names
  • Forkhead activin signal transducer 1
  • Forkhead activin signal transducer 2
  • TGF-beta/activin signal transducer
  • forkhead activin signal transducer-1
  • forkhead box H1
Background InformationForkhead box H1 (FOXH1) is a member of the HNF–3FKH family of transcriptional activators. FOXH1 is a winged helix with two loops on the C-terminal side expressed in all human tissue. FOXH1 is an embryonic transcription regulator and is an essential transcriptional co-activator that binds with SMAD2 to activate activin, and therefore induce the gooseciod (GSC) promoter. After FOXH1 binds to SMAD4, activin response element is activated and TGF-β is stimulated. FOXH1 also interacts with NKX2-5 to form an essential component of anterior heart field development. FOXH1 is associated with complex brain malfunctions due to incomplete cleavage of the prosencephalon, and in developmental delays resulting in facial abnormalities such as cyclopia and severe cleft lip or palate.
References
Product Information
FormatAffinity Purified
Control
  • NIH/3T3, HeLa, and A431 cells.
PresentationPurified rabbit polyclonal antibody precipitated in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.
Quality LevelMQ100
Applications
ApplicationAnti-FOXH1 Antibody is a Rabbit Polyclonal Antibody for detection of FOXH1 also known as Forkhead activin signal transducer, TGF-beta/activin signal transduce & has been validated in ICC.
Key Applications
  • Immunocytochemistry
Biological Information
ImmunogenSynthetic to peptide corresponding to FOXH1.
HostRabbit
SpecificityThis antibody recognizes FOXH1.
Species Reactivity
  • Mouse
  • Human
Species Reactivity NoteHuman. Predicted to react with mouse based on sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • FAST-1
  • FAST1
  • FAST2
  • Fast-1
  • Fast-2
  • hFAST-1
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION:Transcriptional activator. Recognizes and binds to the DNA sequence 5'-TGT[GT][GT]ATT-3'. Required for induction of the goosecoid (GSC) promoter by TGF-beta or activin signaling. Forms a transcriptionally active complex containing FOXH1/SMAD2/SMAD4 on a site on the GSC promoter called TARE (TGF-beta/activin response element). Ref.1

SUBUNIT: Interacts with the MH2 domains of SMAD2 and SMAD3. Ref.1

SUBCELLULAR LOCATION: Nucleus.

TISSUE SPECIFICITY: Ubiquitous. Ref.1

DOMAIN:The FM region is required for binding SMAD2/SMAD4 complexes. FM2 is more effective than FM1 and only interacts with phosphorylated SMAD2 that is in an activated SMAD complex By similarity.

Sequence similarities Contains 1 fork-head DNA-binding domain.
Molecular Weight38 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by immunocytochemistry on NIH/3T3, HeLa, and A431 cells.

Immunocytochemistry:
1:500 dilution of this antibody was used to detect FOXH1 in NIH/3T3, HeLa and A431 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8ºC from date of receipt.
To reconstitute the antibody, centrifuge the antibody at a moderate speed (5,000 rpm) for 5 minutes. Carefully remove the ammonium sulfate/PBS buffer solution and discard; 10μL of residual ammonium sulfate solution will not affect the re-suspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur. Re-suspend the antibody pellet in 100L either standard PBS or TBS (pH 7.3-7.5). DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to re-suspend are normal. Vials are overfilled to compensate for any losses.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
07-1708 04053252324376