Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

ABT11 Anti-Fibroblast activation protein alpha (FAP) Antibody

Detect Fibroblast activation protein alpha (FAP) using this Anti-Fibroblast activation protein alpha (FAP) Antibody validated for use in WB, IH.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure, Apoptosis & Cancer Research Sub-Category: ECM Proteins, Tumor Markers Gene Symbol: FAPA, FAP, DPPIV UniProt Number: Q12884
View Products on Sigmaaldrich.com
ABT11
100 µg  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IHC, FUNCRbPurifiedPolyclonal Antibody
Description
Catalogue NumberABT11
DescriptionAnti-Fibroblast activation protein alpha (FAP) Antibody
Alternate Names
  • fibroblast activation protein, alpha
  • fibroblast activation protein, alpha subunit
  • integral membrane serine protease
  • seprase
  • 170 kDa melanoma membrane-bound gelatinase, FAP
Background InformationFibroblast activation protein alpha (FAP), also known as Seprase, is a 97 kDa, type II cell surface glycoprotein belonging to the serine protease family. Although FAP is considered fibroblast specific, it is not found in normal healthy fibroblasts. FAP is most commonly found in stromal, tumor fibroblasts, and is likely involved in ECM pericellular proteolysis, endothelial cell migration and invasion into the ECM. The presence of FAP in human epithelial carcinoma is increased, particularly in breast, colorectal, lung, and ovarian carcinomas. FAP is also transiently observed in developing cells, or in tissues that are undergoing wound healing or suffering from chronic inflammatory conditions. Due to its specific distribution both to tumor fibroblasts and to inflammed tissue, FAP makes a promising target for research in tumor-stromal studies and in therapeutic studies.
References
Product Information
FormatPurified
Control
  • HEK-FAP xenograft tumor lysate
PresentationPurified rabbit polyclonal in buffer containing PBS (pH 7.2) with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect Fibroblast activation protein alpha (FAP) using this Anti-Fibroblast activation protein alpha (FAP) Antibody validated for use in WB, IH.
Key Applications
  • Western Blotting
  • Immunohistochemistry
  • Affects Function
Application NotesImmunohistochemistry: A 1:750 dilution of a previous lot of FAP antibody was used to immunohistochemically stain frozen tissue sections of HEK-FAP or HT-29 xenograft tumors from C.B17 mice (Cheng, 2002).

Inhibition: Inhibition of dipeptidyl peptidase activity with a previous lot of FAP antibody on HT-29 xenograft tumors was demonstrated by an independent laboratory (Cheng, 2002).
Biological Information
ImmunogenHistidine-tagged recombinant protein corresponding to human FAP.
EpitopeExtracellular domain
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThe antibody recognizes the extracellular domain of Fibroblast activation protein alpha
IsotypeIgG
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. It is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. This protein is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis. [provided by RefSeq].
Gene Symbol
  • FAPA
  • FAP
  • DPPIV
Purification MethodAmmonium Sulfate
UniProt Number
UniProt SummaryFUNCTION: In association with DPP4 is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May have a role in tissue remodeling during development and wound healing, and may contribute to invasiveness in malignant cancers.

CATALYTIC ACTIVITY: Degrades gelatin and heat-denatured type I and type IV collagen, but not native type I or type IV collagen. Does not cleave laminin, fibronectin, fibrin or casein.

SUBUNIT STRUCTURE: Homodimer, or heterodimer with DPP4. The monomer is inactive.

SUBCELLULAR LOCATION: Cell membrane; Single-pass type II membrane protein. Cell projection › lamellipodium membrane; Single-pass type II membrane protein. Cell projection › invadopodium membrane; Single-pass type II membrane protein. Note: Found in cell surface lamellipodia, invadopodia and on shed vesicles. Colocalized with DPP4 in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Colocalized with DPP4 on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma.

TISSUE SPECIFICTY: Fibroblast specific.

INDUCTION: In fibroblasts at times and sites of tissue remodeling during development, tissue repair, and carcinogenesis.

PTM: N-glycosylated. Ref.11 Ref.9

The N-terminus may be blocked.

SEQUENCE SIMILARITIES: Belongs to the peptidase S9B family.
Molecular Weight~ 90 kDa observed MW. Two isoforms of the Fibroblast activation protein exist at calculated MWs of 88kDa and 27 kDa. It is possible that both bands may be obserbved in certain cell lines.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in HEK-FAP xenograft tumor lysate.

Western Blot Analysis: 2.5 µg/mL of this antibody detected FAP in 10 µg of HEK-FAP xenograft tumor lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABT11 04053252421778

Documentation

Anti-Fibroblast activation protein alpha (FAP) Antibody MSDS

Title

Safety Data Sheet (SDS) 

Anti-Fibroblast activation protein alpha (FAP) Antibody Certificates of Analysis

TitleLot Number
Anti-Fibroblast activation protein alpha (FAP) 3063843
Anti-Fibroblast activation protein alpha (FAP) 2475938
Anti-Fibroblast activation protein alpha (FAP) - 2127825 2127825
Anti-Fibroblast activation protein alpha (FAP) - 2327011 2327011
Anti-Fibroblast activation protein alpha (FAP) - 1987098 1987098
Anti-Fibroblast activation protein alpha (FAP) - 2150749 2150749
Anti-Fibroblast activation protein alpha (FAP) - 2207216 2207216
Anti-Fibroblast activation protein alpha (FAP) - 2216893 2216893
Anti-Fibroblast activation protein alpha (FAP) - 2239626 2239626
Anti-Fibroblast activation protein alpha (FAP) - 2280473 2280473
Show more