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MABT409 Anti-Integrin Beta1, clone AIIB2 (Azide Free) Antibody

This Anti-Integrin Beta1, clone AIIB2 (Azide Free) Antibody is validated for use in Western Blotting and Immunocytochemistry and Activity Assay and Immunoprecipitation and Immunohistochemistry for the detection of Integrin Beta1.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Adhesion (CAMs) Gene Symbol: ITGB1, FNRB, MDF2, MSK12 UniProt Number: P05556
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MABT409
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, B, Po, Sh, Eq, Ca, Rhesus MacaqueWB, ICC, ACT, IP, IHCRPurifiedMonoclonal Antibody
Description
Catalogue NumberMABT409
DescriptionAnti-Integrin Beta1, clone AIIB2 (Azide Free) Antibody
Alternate Names
  • Integrin beta-1
  • Fibronectin receptor subunit beta
  • Glycoprotein Iia
  • GPIIA
  • VLA-4 subunit beta
  • CD29
Background InformationIntegrin beta-1, also known as Fibronectin receptor subunit beta (FNRB), and Glycoprotein IIa (GPIIA), is a transmembrane glycoprotein that forms noncovalent complexes with various alpha integrin subunits to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metatastatic diffusion of tumor cells. Interactions between integrins and extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression. Phosphorylation of threonines 788 and 789 on integrin beta1 receptor may play a key role in cell-cycle dependent regulation.
References
Product Information
FormatPurified
PresentationPurified rat monoclonal IgG1κ in buffer containing PBS without preservatives.
Quality LevelMQ100
Applications
ApplicationThis Anti-Integrin Beta1, clone AIIB2 (Azide Free) Antibody is validated for use in Western Blotting and Immunocytochemistry and Activity Assay and Immunoprecipitation and Immunohistochemistry for the detection of Integrin Beta1.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Activity Assay
  • Immunoprecipitation
  • Immunohistochemistry
Application NotesActivity Assay Analysis: A representative lot detected Integrin Beta1, clone AIIB2 (Azide Free) in this functionally blocking antibody abrogated AGS cell attachment to fibronectin while causing only a slight inhibition of cell attachment to vitronectin (Hutton, M., et. al. (2010). Infection and Immunity. 78(11):4523-4531).
Immunohistochemistry Analysis: A representative lot detected Integrin Beta1, clone AIIB2 (Azide Free) in floating villi tissue (Damsky, C. H., et al. (1992). Journal of Clinical Investigation. 89:210-222).
Immunohistochemistry Analysis: A representative lot detected Integrin Beta1, clone AIIB2 (Azide Free) in CTB aggregates that have invaded matrigel in vitro (Damsky, C. H., et al. (1994). Development. 120:3657-3666).
Immunocytochemistry Analysis: A representative lot detected Integrin Beta1, clone AIIB2 (Azide Free) in AGS cells (Hutton, M., et. al. (2010). Infection and Immunity. 78(11):4523-4531).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Integrin Beta1, clone AIIB2 (Azide Free) in NP-40 cell lysate (Werb, Z., et al. (1989). JCB. 109:877-889).
Biological Information
ImmunogenWhole cells corresponding to human Integrin Beta1, clone AIIB2 (Azide Free).
CloneAIIB2
Concentration1 mg/mL
HostRat
IsotypeIgG1κ
Species Reactivity
  • Human
  • Bovine
  • Pig
  • Sheep
  • Horse
  • Canine
  • Rhesus Macaque
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • ITGB1
  • FNRB
  • MDF2
  • MSK12
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~110 kDa observed. The calculated molecular weight of this protein is 88 kDa, but can be observed at ~110 kDa due to high glycosylation.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HUVEC cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Integrin Beta1, clone AIIB2 (Azide Free) in 10 µg of HUVEC cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABT409 04053252993138

Documentation

Anti-Integrin Beta1, clone AIIB2 (Azide Free) Antibody MSDS

Title

Safety Data Sheet (SDS) 

Anti-Integrin Beta1, clone AIIB2 (Azide Free) Antibody Certificates of Analysis

TitleLot Number
Anti-Integrin Beta1, clone AIIB2 (Azide -2545775 2545775
Anti-Integrin Beta1, clone AIIB2 (Azide -2548075 2548075
Anti-Integrin Beta1, clone AIIB2 (Azide -2550072 2550072
Anti-Integrin Beta1, clone AIIB2 (Azide -2665829 2665829
Anti-Integrin Beta1, clone AIIB2 (Azide -2794967 2794967
Anti-Integrin Beta1, clone AIIB2 (Azide Free) 3079120
Anti-Integrin Beta1, clone AIIB2 (Azide Free) - Q2452912 Q2452912
Anti-Integrin Beta1, clone AIIB2 (Azide Free) - 2888556 2888556
Anti-Integrin Beta1, clone AIIB2 (Azide Free) - 3194631 3194631
Anti-Integrin Beta1, clone AIIB2 (Azide Free) - 3232379 3232379
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