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ABE1851 Anti-MLL-3

Anti-MLL3, Cat. No. ABE1851, is a rabbit polyclonal antibody that detects Myeloid-lineage leukemia protein 3 homolog (MLL3) and is tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

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ABE1851
100 μg  
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Overview

Replacement Information
Description
Catalogue NumberABE1851
DescriptionAnti-MLL-3
Alternate Names
  • HALR
  • Histone-lysine N-methyltransferase 2C
  • EC: 2.1.1.43
  • Lysine-N-methyltransferase 2C
  • Myeloid-lineage leukemia protein 3 homolog
Background InformationHistone-lysine N-methyltransferase 2C (UniProt: Q8BRH4; also known as EC:2.1.1.354, Lysine N-methyltransferase 2C, Myeloid/lymphoid or mixed-lineage leukemia protein 3 homolog) is encoded by the Kmt2c (also known as Mll3) gene (Gene ID: 231051) in murine species. MLL 3 is a histone methyltransferase that catalyzes the methyl group transfer from S-adenosyl-L-methionine to the epsilon-amino group of lysine 4 of histone H3 (H3K4). It is a part of chromatin remodeling machinery that predominantly forms H3K4me1 methylation marks at active chromatin sites where transcription and DNA repair take place. It is a DHHC-type zinc finger protein with eight PHD-type zinc fingers, a SET domain (aa 4763-4879), a post-SET domain (aa 4887-4903) and a RING-type zinc finger (aa 343-388). The SET domain interacts with histone H3, but not H2A, H2B, and H4. In adults, MLL-3 is detected in testis, kidney, spleen, and lung and is weakly expressed in brain. It is absent in heart and liver tissue. Mll3 gene is reported to be frequently mutated in cancers and shows a high frequency of loss-of-function mutations, which are spread over the entire protein, including the PHD fingers and SET domain. Mutations in Mll3 gene are reported to cause defects in BRCA1 associated protein 1 (BAP1) recruitment to the enhancers of tumor-suppressor genes, which represses enhancer activity and promotes tumorigenesis. Depletion of MLL3 can enhance cell migration, but does not elevate the proliferation rate of cancer cells. (Ref.: Zheng, J-Y., et al. (2021). Cell Death Dis. 12(4); Article 364).
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-MLL3, Cat. No. ABE1851, is a rabbit polyclonal antibody that detects Myeloid-lineage leukemia protein 3 homolog (MLL3) and is tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Key Applications
  • Immunohistochemistry (Paraffin)
  • Immunocytochemistry
  • Western Blotting
Application NotesTested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:250-1,000 dilution from a representative lot detected MLL3 in mouse testis and mouse kidney tissue sections.

Western Blotting Analysis: A 1:500 dilution from a representative lot detected mouse recombinant MLL3.

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected MLL3 in U2OS cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 18 amino acids from the N-terminal half of murine Myeloid/lymphoid or mixed-lineage leukemia protein 3 homolog (MLL3).
Epitopeunknown
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody specifically detects Myeloid/lymphoid or mixed-lineage leukemia protein 3 homolog (MLL3). It targets an epitope within 18 amino acids from the N-terminal half.
IsotypeIgG
Species Reactivity
  • Mouse
  • Rat
  • Human
Species Reactivity NoteHuman, Mouse, Rat.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • Kmt2C
  • Mll3
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~540 kDa observed; 540.19 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in rat uterus tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution of this antibody detected MLL3 in rat uterus tissue sections.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABE1851 04054839233210

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