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ABN76 Sigma-AldrichAnti-Neurofilament H (200 kDa) Antibody

This Anti-Neurofilament H (200 kDa) Antibody is validated for use in WB, IH(P) for the detection of Neurofilament H (200 kDa).

Anti-Neurofilament H (200 kDa) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Research Category: Neuroscience Research Sub-Category: Neurofilament & Neuron Metabolism Gene Symbol: NEFH UniProt Number: P12036

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Key Spec Table

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, B, R	WB, IH(P)	Rb	Affinity Purified	Polyclonal Antibody

Description
Catalogue Number	ABN76
Replaces	AB1982
Description	Anti-Neurofilament H (200 kDa) Antibody
Alternate Names	Neurofilament heavy polypeptide Neurofilament H
Background Information	Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexes of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas, adrenal and extra-adrenal pheochromocytomas, carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung. For moreinformation, see the Nervous System Cell Type Specific Marker chart on the Millipore website.

References

Product Information
Format	Affinity Purified
Control	Bovine cerebellum tissue lysate
Presentation	Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	This Anti-Neurofilament H (200 kDa) Antibody is validated for use in WB, IH(P) for the detection of Neurofilament H (200 kDa).
Key Applications	Western Blotting Immunohistochemistry (Paraffin)
Application Notes	Immunohistochemistry Analysis: A 1 µg/mL dilution from a representative lot detected Neurofilament H in normal rat cerebellum tissue.

Biological Information
Immunogen	GST-tagged recombinant protein corresponding to bovine Neurofilament H.
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Species Reactivity	Human Mouse Bovine Rat
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_066554.2
Entrez Gene Summary	Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene.
Gene Symbol	NEFH
Purification Method	Affinity Purfied
UniProt Number	P12036
UniProt Summary	FUNCTION: Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins. POST-TRANSLATION MODIFICATION: Modification There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function. Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization. POLYMORPHISM: The number of repeats is shown to vary between 29 and 30. INVOLVEMENT IN DISEASE: Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors. SEQUENCE SIMILARITIES: Belongs to the intermediate filament family. SEQUENCE CAUTION: The sequence BAA74868.2 differs from that shown. Reason: Erroneous initiation. The sequence BAG63896.1 differs from that shown. Reason: Erroneous initiation.
Molecular Weight	~200 kDa observed

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in bovine cerebellum tissue lysate. Western Blot Analysis: 0.1 µg/mL of this antibody detected Neurofilament H in bovine cerebellum tissue lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
ABN76	04053252632051

Documentation

Anti-Neurofilament H (200 kDa) Antibody MSDS

Title
Safety Data Sheet (SDS)

Anti-Neurofilament H (200 kDa) Antibody Certificates of Analysis

Title	Lot Number
Anti-Neurofilament H (200 kDa) - 2458003	2458003
Anti-Neurofilament H (200 kDa) - 1966930	1966930
Anti-Neurofilament H (200 kDa) - 2211976	2211976
Anti-Neurofilament H (200 kDa) - 2275638	2275638
Anti-Neurofilament H (200 kDa) - 2290993	2290993
Anti-Neurofilament H (200 kDa) - 3202219	3202219
Anti-Neurofilament H (200 kDa) - 3388678	3388678
Anti-Neurofilament H (200 kDa) - 3783582	3783582
Anti-Neurofilament H (200 kDa) - 3862691	3862691
Anti-Neurofilament H (200 kDa) - 3988985	3988985

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AB1989	Anti-Neurofilament H (200 kDa) Antibody, CT
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AB5539	Anti-Neurofilament H Antibody
CBL212	Anti-Neurofilament 200 kDa Antibody, clone RT97
MAB5256	Anti-Neurofilament 200 kDa Antibody, clone NE14
MAB5262	Anti-Neurofilament 200 kDa Antibody, clone RT97
MAB5266	Anti-Neurofilament 200 kDa Antibody, clone N52
MAB5446	Anti-Neurofilament NF-H Antibody, tail domain (Phosphate dependent), clone RMO-24
MAB5448	Anti-Neurofilament NF-H Antibody, clone TA51

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