Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

CB1044 Anti-OCLN Mouse mAb (1G7)

This Anti-OCLN Mouse mAb (1G7) is validated for use in ELISA, Immunoblotting, Immunocytochemistry for the detection of OCLN.

Anti-OCLN Mouse mAb (1G7) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Occludin

View Products on Sigmaaldrich.com
CB1044
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
CB1044-100UGCN 100 μg
Description
OverviewRecognizes the ~55-60 kDa OCLN protein in HL-60 and HeLa cells.
Catalogue NumberCB1044
Brand Family Calbiochem®
SynonymsAnti-Occludin
Application Data
Detection of human OCLN by immunoblotting. Sample: Whole cell lysate from HL-60 cells. Primary antibody: Anti-OCLN Mouse mAb (1G7) (Cat. No. CB1044) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human OCLN by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-OCLN Mouse mAb (1G7) (Cat. No. CB1044) (10 µg/ml). Detection: fluorescence.
References
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
Positive controlHL-60 cells, HeLa cells
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunocytochemistry
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenA recombinant polypeptide corresponding to amino acids of 423-523 human OCLN, expressed as a GST fusion protein
Clone1G7
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
CB1044-100UGCN 04055977228403

Documentation

Anti-OCLN Mouse mAb (1G7) MSDS

Title

Safety Data Sheet (SDS) 

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision01-November-2010 RFH
SynonymsAnti-Occludin
ApplicationELISA (see comments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Application Data
Detection of human OCLN by immunoblotting. Sample: Whole cell lysate from HL-60 cells. Primary antibody: Anti-OCLN Mouse mAb (1G7) (Cat. No. CB1044) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human OCLN by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-OCLN Mouse mAb (1G7) (Cat. No. CB1044) (10 µg/ml). Detection: fluorescence.
DescriptionPurified mouse monoclonal antibody. Recognizes the ~55-60 kDa OCLN protein.
BackgroundOCLN is an integral membrane protein which is located at tight junctions. This protein may be involved in the formation and maintenance of the tight junction.
HostMouse
ImmunogenA recombinant polypeptide corresponding to amino acids of 423-523 human OCLN, expressed as a GST fusion protein
Clone1G7
IsotypeIgG₁
Specieshuman
Positive controlHL-60 cells, HeLa cells
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review