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MABS1721 Anti-Potassium Channel Kir3.4 Antibody, clone 8F4.1

Detect K(+) channel Kir3.4 using this mouse monoclonal Anti-Potassium Channel Kir3.4, clone 8F4.1, Cat. No. MABS1721, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: KCNJ5, GIRK4, LQT13 UniProt Number: P48544
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MABS1721
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS1721
DescriptionAnti-Potassium Channel Kir3.4 Antibody, clone 8F4.1
Alternate Names
  • G protein-activated inward rectifier potassium channel 4
  • Cardiac inward rectifier
  • CIR
  • Heart KATP channel
  • Inward rectifier K(+) channel Kir3.4
  • GIRK-4
  • IRK-4
  • KATP-1
  • Potassium channel
  • inwardly rectifying subfamily J member 5
Background InformationG protein-activated inward rectifier potassium channel 4 (UniProt P48544; also known as Cardiac inward rectifier, CIR, Heart KATP channel, Inward rectifier K(+) channel Kir3.4, GIRK-4, IRK-4, KATP-1, Potassium channel, inwardly rectifying subfamily J member 5) is encoded by the KCNJ5 (also known as GIRK4, LQT13) gene (Gene ID 3762) in human. Inward rectifier K+ channels (Kirs) modulate cell membrane potential and are classified as K+ transport channels (Kir1.x, Kir4.x, Kir5.x, Kir7.x), classic Kir (Kir2.x), G-protein-gated Kirs (Kir2.x and Kir3.x), and ATP-sensitive Kirs (Kir6.x). Kirs mediate diverse physiological functions of both excitable and nonexcitable epithelial cells, such as cell adhesion-migration, volume regulation, apoptosis, and proliferation. While a large fraction of Kir3.4 (GIRK4; KCNJ5) subunits is processed to the cell membrane to form homomultimers, Kir3.4 subunits also assemble into heteromeric channels with Kir3.1 (GIRK1; KCNJ3) subunits in atrial myocytes or Kir3.2 (GIRK2; KCNJ6) in the cerebellum. Upon agonist activation of appropriate GPCRs, heteroterameric Kir3.4 channels are opened via interaction with subunits of the pertussis toxin-sensitive class (Gi/o) G-proteins, resulting in membrane hyperpolarization and/or reduced excitability. Whereas homotetrameric Kir3.4 channels can be activated by high intracellular Na+ independent of G-protein. Human Kir3.4 is a 419-amino acid multi-pass membrane (a.a. 87-111, 136-147, 164-185) protein that contains two small extraceullar regions (a.a. 112-135 and 155-163) and a pore-forming domain (a.a. 148-154), having both its N- and C-terminal ends exposed intracellularly (a.a. 1-86 and a.a. 186-419).
References
Product Information
FormatPurified
PresentationPurified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect K(+) channel Kir3.4 using this mouse monoclonal Anti-Potassium Channel Kir3.4, clone 8F4.1, Cat. No. MABS1721, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected potassium channel Kir3.4 in human adrenal gland and kidney tissue sections.
Biological Information
ImmunogenGST-tagged human potassium channel Kir3.4 C-terminal end fragment.
Clone8F4.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 8F4.1 detects human potassium channel Kir3.4 by targeting an epitope within the C-terminal cytoplasmic domain.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Specific ActivityC-terminal region.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • KCNJ5
  • GIRK4
  • LQT13
Purification MethodProtein G purified
UniProt Number
Molecular Weight~47 kDa observed. 47.67 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human adrenal gland tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected potassium channel Kir3.4 in 10 µg of human adrenal gland tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABS1721 04054839081330