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MABC684 Anti-SOD1 Antibody, clone 6F5

Anti-SOD1 Antibody, clone 6F5 is a highly specific mouse monoclonal antibody, that targets Superoxide Dismutase & has been tested in western blotting, ICC & Flow Cytometry.

Anti-SOD1 Antibody, clone 6F5 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: SOD1 UniProt Number: P00441
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MABC684
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, ICC, FCMAscitesMonoclonal Antibody
Description
Catalogue NumberMABC684
DescriptionAnti-SOD1 Antibody, clone 6F5
Alternate Names
  • ALS
  • SOD
  • ALS1
  • IPOA
  • homodimerSuperoxide dismutase [Cu-Zn]
  • Superoxide dismutase 1
  • hSod1
Background InformationSuperoxide dismutase 1 (SOD1) also known as Superoxide dismutase [Cu-Zn] to distinguish it from SOD2 which uses manganese as its cofactor, is the cytosolic form of the enzyme that catalyzes the breakdown of superoxide into oxygen and hydrogen peroxide and is our primary anti-oxidant combating enzyme. SOD1 performs its function with the help of co-factors and one protein in particular is vital to its proper functioning. Copper chaperone protein (CCS) is a metalloprotein that specifically delivers Cu to SOD1 and may activate SOD1 through direct insertion of the Cu cofactor. Interestingly, defects in SOD1 are the cause of a form of ALS (Amyotrophic Lateral Sclerosis or Lou Gehrig's disease) and much research is centered on understanding how over-active or under-active SOD1 contributes to the disease. EMD-Millipore's Anti-SOD1 mouse monoclonal antibody has been tested in western blot on HeLa, NIH3T3, A549 and A431 cell lysates and fluorescent immunocytochemistry on PANC1, SKBR-3 and 3T3-L1 cells in culture. The antibody has also been tested in flow cytometry on A431 cells in culture.
References
Product Information
FormatAscites
Control
  • HeLa, NIH/3T3, A549, and A431 cell lysates
PresentationMouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-SOD1 Antibody, clone 6F5 is a highly specific mouse monoclonal antibody, that targets Superoxide Dismutase & has been tested in western blotting, ICC & Flow Cytometry.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Flow Cytometry
Application NotesImmunocytochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected SOD1 in PANC-1, SKBR-3, and 3T3-L1 cells.

Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected SOD1 in non serum starved A431 cells.

Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenPurified recombinant fragment of human SOD1 expressed in E. Coli.
Clone6F5
HostMouse
IsotypeIgG1
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Gene Symbol
  • SOD1
Purification MethodUnpurified
UniProt Number
Molecular Weight~18 kDa observed. Uncharacterized bands may appear in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa, NIH/3T3, A549, and A431 cell lysates.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected SOD1 in HeLa, NIH/3T3, A549, and A431 cell lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABC684 04053252918063

Documentation

Anti-SOD1 Antibody, clone 6F5 MSDS

Title

Safety Data Sheet (SDS) 

Anti-SOD1 Antibody, clone 6F5 Certificates of Analysis

TitleLot Number
Anti-SOD1, clone 6F5 VP1406129
Anti-SOD1, clone 6F5 - VP1402183 VP1402183
Anti-SOD1, clone 6F5 - QVP1305200 QVP1305200
Anti-SOD1, clone 6F5 - VP1310030 VP1310030
Anti-SOD1, clone 6F5 - VP2204221 VP2204221
Anti-SOD1, clone 6F5 -VP1509047 VP1509047
Anti-SOD1, clone 6F5 -VP1509242 VP1509242
Anti-SOD1, clone 6F5 -VP1511206 VP1511206
Anti-SOD1, clone 6F5 -VP1601124 VP1601124
Anti-SOD1, clone 6F5 -VP1607198 VP1607198
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Brochure

Title
Pathways and Biomarkers of Oxidative Stress

Technical Info

Title
White Paper- Modern Methods in Oxidative Stress Research