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07-2173 Anti-Stat3 Antibody

Use Anti-Stat3 Antibody (rabbit polyclonal antibody) validated in WB, IP to detect Stat3 also known as Acute-phase response factor, DNA-binding protein APRF, signal transducer & activator of transcription 3.

Anti-Stat3 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Transcription Factors Gene Symbol: APRF, HIES, STAT3 UniProt Number: P40763
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07-2173
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, R, B, Po, PmWB, IPRbPurifiedPolyclonal Antibody
Description
Catalogue Number07-2173
ReplacesAB3162
DescriptionAnti-Stat3 Antibody
Alternate Names
  • Acute-phase response factor
  • DNA-binding protein APRF
  • signal transducer and activator of transcription 3
  • signal transducer and activator of transcription 3
  • (acute-phase response factor)
Background InformationThis protein is a member of the family of STAT proteins (Signal Transduction and Activators of Transcription). These proteins are latent cytoplasmic transcription factors with the dual function of signal transduction and activation of transcription. STATs are activated by tyrosine phosphorylation in response to different ligands, after which they translocate to the cell nucleus. The N-terminal region is highly homologous among the STAT proteins and surrounds a completely conserved arginine residue. The Stat3 protein is expressed in most cell types and is activated by multiple cytokines and growth factors including: IFN-a, IL-10, IL-6, IL-11, onM, LIF, CNTF, G-CSF, IL-12, IL-2, GH, EGF, PDGF, and CSF-1. Stat3 is not activated by IFN beta. One active Stat3 translocates to the nucleus and binds to DNA as either a homodimer or as a heterodimer with Stat1. Transcription factors associated with Stat3 include cJun and cyclic AMP responsive enhancer binding protein (CREB). Stat3 interacts with the APRE (acute phase response element) and the SIE (c-sis inducible element). These proteins are latent cytoplasmic transcription factors with the dual function of signal transduction and activation of transcription. STATs are activated by tyrosine phosphorylation in response to different ligands, after which they translocate to the cell nucleus. The N-terminal region is highly homologous among the STAT proteins and surrounds a completely conserved arginine residue.

References
Product Information
FormatPurified
Control
  • HeLa cell lysate
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationUse Anti-Stat3 Antibody (rabbit polyclonal antibody) validated in WB, IP to detect Stat3 also known as Acute-phase response factor, DNA-binding protein APRF, signal transducer & activator of transcription 3.
Key Applications
  • Western Blotting
  • Immunoprecipitation
Application NotesWestern Blot (SNAP ID) Analysis: 5 µg/mL from a representative lot detected STAT3 on 10 µg of HeLa cell lysate.

Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated STAT3 from 500 µg of HeLa RIPA lysate.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human STAT3 near the C-terminus.
EpitopeNear C-terminus
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody recognizes STAT3 near the C-terminus.
Species Reactivity
  • Human
  • Mouse
  • Rat
  • Bovine
  • Pig
  • Primate
Species Reactivity NoteDemonstrated to react with human, mouse, and rat. Predicted to react with bovine, porcine, and primate based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq].
Gene Symbol
  • APRF
  • HIES
  • STAT3
Purification MethodProtein A
UniProt Number
UniProt SummaryFUNCTION: Transcription factor that binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA.

SUBUNIT STRUCTURE: Forms a homodimer or a heterodimer with a related family member (at least STAT1). Interacts with NCOA1, PELP1, SOCS7 and STATIP1. Interacts with HCV core protein. Interacts with IL23R in presence of IL23. Interacts with IL31RA. Interacts with SIPAR. Interacts (via SH2 domain) with NLK By similarity. Interacts with KPNA4 and KPNA5; KPNA4 may be the primary mediator of nuclear import By similarity. Interacts with TMF1.

SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note: Shuttles between the nucleus and the cytoplasm. Constitutive nuclear presence is independent of tyrosine phosphorylation.

TISSUE SPECIFICITY: Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

PTM: Tyrosine phosphorylated in response to IL-6, IL-11, CNTF, LIF, CSF-1, EGF, PDGF, IFN-alpha and OSM. Phosphorylated on serine upon DNA damage, probably by ATM or ATR. Serine phosphorylation is important for the formation of stable DNA-binding STAT3 homodimers and maximal transcriptional activity.

INVOLVEMENT IN DISEASE: Defects in STAT3 are the cause of hyperimmunoglobulin E recurrent infection syndrome autosomal dominant (AD-HIES) [MIM:147060]; also known as hyper-IgE syndrome or Job syndrome. AD-HIES is a rare disorder of immunity and connective tissue characterized by immunodeficiency, chronic eczema, recurrent Staphylococcal infections, increased serum IgE, eosinophilia, distinctive coarse facial appearance, abnormal dentition, hyperextensibility of the joints, and bone fractures.

MISCELLANEOUS: Involved in the gp130-mediated signaling pathway.

SEQUENCE SIMILARITIES: Belongs to the transcription factor STAT family.

Contains 1 SH2 domain.
Molecular Weight~ 88 kDa observed.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected STAT3 on 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
07-2173 04053252375316

Documentation

Anti-Stat3 Antibody MSDS

Title

Safety Data Sheet (SDS) 

Anti-Stat3 Antibody Certificates of Analysis

TitleLot Number
Anti-Stat3 - 2394071 2394071
Anti-Stat3 - 2430543 2430543
Anti-Stat3 - 2438700 2438700
Anti-Stat3 - 2007345 2007345
Anti-Stat3 - 2343898 2343898
Anti-Stat3 - 2514243 2514243
Anti-Stat3 - 3432458 3432458
Anti-Stat3 - 3848486 3848486
Anti-Stat3 - 4169256 4169256
Anti-Stat3 - NG1861050 NG1861050
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Brochure

Title
Pathways and Biomarkers of JAK/STAT Signaling

Technical Info

Title
JAK/STAT Signaling Research Focus