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07-030-AF647 Anti-dimethyl Histone H3 Antibody (Lys4), Alexa Fluor® 647 Conjugate

This Anti-dimethyl Histone H3 Antibody (Lys4), Alexa Fluor® 647 Conjugate is validated for use in Immunocytochemistry for the detection of dimethyl Histone H3.

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Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones Gene Symbol: HIST1H3A, H3FA, HIST1H3B, H3FB, HIST1H3C, H3FC, HIST1H3D, H3FD, HIST1H3E, HIST1H3F, H3FF, HIST1H3G, HIST1H3H, H3FH, HIST1H3I, H3FI, HIST1H3J, H3FJ, H3FK, HIST3H3, H3FT, H3T UniProt Number: Q16695
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07-030-AF647
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, TICCRbPurifiedPolyclonal Antibody
Description
Catalogue Number07-030-AF647
DescriptionAnti-dimethyl Histone H3 Antibody (Lys4), Alexa Fluor® 647 Conjugate
Alternate Names
  • H3K4me2
  • H3.3B, Lys4 dimethylated
  • H3 histone, family 3A, Lys4 dimethylated
  • H3 histone, family 3B, Lys4 dimethylated
  • Histone H3, Lys4 dimethylated
Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci.
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-dimethyl Histone H3 Antibody (Lys4), Alexa Fluor® 647 Conjugate is validated for use in Immunocytochemistry for the detection of dimethyl Histone H3.
Key Applications
  • Immunocytochemistry
Application NotesThe unconjugated antiserum (Cat. No. 07-030) is shown to be suitable also for dot blot, chromatin immunoprecipitation (ChIP), ChIP-seq, immunocytochemistry, immunofluorescence, and Western blotting applications.
Biological Information
ImmunogenKLH-conjugated synthetic peptide (ART[Kme2]QTAR-GC) corresponding to amino acids 1-8 of human histone H3.
EpitopeH3K4me2
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificitySpecificity of this polyclonal antibody toward histone H3 Lys4 dimethylation was demonstrated by dot blot analysis using the unconjugated antiserum (Cat. No. 07-030).
Species Reactivity
  • Human
  • Tetrahymena
Species Reactivity NoteHuman, Tetrahymena. Broad species cross-reactivity is expected.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • HIST1H3A
  • H3FA
  • HIST1H3B
  • H3FB
  • HIST1H3C
  • H3FC
  • HIST1H3D
  • H3FD
  • HIST1H3E
  • HIST1H3F
  • H3FF
  • HIST1H3G
  • HIST1H3H
  • H3FH
  • HIST1H3I
  • H3FI
  • HIST1H3J
  • H3FJ
  • H3FK
  • HIST3H3
  • H3FT
  • H3T
Modifications
  • Methylation
Purification MethodProtein A purified.
UniProt Number
Molecular Weight~17 kDa observed.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected nuclear histone H3 Lys4 dimethylation in A431 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
07-030-AF647 04055977355581