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07-571-I Anti-nNOS/NOS I Antibody

Anti-nNOS/NOS I Antibody is an antibody against nNOS/NOS I for use in western blotting & IHC (Paraffin).

Anti-nNOS/NOS I Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Neurodegenerative Diseases Gene Symbol: Nos1, Bnos UniProt Number: P29476
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07-571-I
200 µL  
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Overview

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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
R, MWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number07-571-I
Replaces07-571
DescriptionAnti-nNOS/NOS I Antibody
Alternate Names
  • Nitric oxide synthase, brain
  • bNOS
  • Constitutive NOS
  • NC-NOS
  • NOS type I
  • Neuronal NOS
  • N-NOS
  • nNOS
  • Peptidyl-cysteine S-nitrosylase NOS1
Background InformationNitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. nNOS/NOS I, also known as NC-NOS, or NOS type I, or Neuronal NOS, or Peptidyl-cysteine S-nitrosylase NOS1 and bNOS produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. NOS I has been suggested to have nitrosylase activity and to mediate cysteine S-nitrosylation of cytoplasmic target proteins such SRR. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. Human iNOS is regulated by calcium/calmodulin (in contrast with mouse NOS2).
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-nNOS/NOS I Antibody is an antibody against nNOS/NOS I for use in western blotting & IHC (Paraffin).
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected nNOS/NOS I in rat cerebellum and cerebral cortex tissues, and mouse hippocampus and thalamus tissues.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the near N-terminus of rat nNOS/NOS I.
EpitopeNear N-terminus
Concentration0.5 mg/mL
HostRabbit
Species Reactivity
  • Rat
  • Mouse
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • Nos1
  • Bnos
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~165 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in rat brain microsomal preparation.

Western Blotting Analysis: A 1:500 dilution of this antibody detected nNOS/NOS I in 10 µg of rat brain microsomal preparation.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size200 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
07-571-I 04053252972881

Documentation

Anti-nNOS/NOS I Antibody MSDS

Title

Safety Data Sheet (SDS) 

Anti-nNOS/NOS I Antibody Certificates of Analysis

TitleLot Number
Anti-nNOS/NOS I - Q2411270 Q2411270
Anti-nNOS/NOS I - 3013394 3013394
Anti-nNOS/NOS I - 3386855 3386855
Anti-nNOS/NOS I - 3519341 3519341
Anti-nNOS/NOS I - 3724658 3724658
Anti-nNOS/NOS I - 3807983 3807983
Anti-nNOS/NOS I - 3898661 3898661
Anti-nNOS/NOS I - 4006701 4006701
Anti-nNOS/NOS I - 4128267 4128267
Anti-nNOS/NOS I - 4279409 4279409
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Brochure

Title
Pathways and Biomarkers of Oxidative Stress

Technical Info

Title
White Paper- Modern Methods in Oxidative Stress Research