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05-636-AF647 Anti-phospho Histone H2A.X (Ser139) Antibody, clone JBW301, Alexa Fluor® 647

This Anti-phospho Histone H2A.X (Ser139) Antibody, clone JBW301, Alexa Fluor® 647 is validated for use in ICC for the detection of phospho Histone H2A.X (Ser139).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones Gene Symbol: H2AFX, H2AX UniProt Number: P16104
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05-636-AF647
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HICCMAlexaFluor®647Monoclonal Antibody
Description
Catalogue Number05-636-AF647
DescriptionAnti-phospho Histone H2A.X (Ser139) Antibody, clone JBW301, Alexa Fluor® 647
Alternate Names
  • Histone H2AX
  • H2a/x
  • Histone H2A.X
Background InformationAs a member of the histone H2A family, histone H2A.x (H2A.x) is a variant histone H2A which replaces conventional H2A in a subset of nucleosomes. H2A.x is involved in the DNA repair of double-strand breakage (DSB) damage on nuclear DNA. After a double strand DNA break, H2A.x is rapidly phosphorylated at serine 139 by ATM kinase and becomes gamma-H2AFX. This phosphorylation step can extend up to several thousand nucleosomes from the actual site of the DSB and may mark the surrounding chromatin for recruitment of proteins required for DNA damage signaling and repair. As a part of posttranslational modifications during apoptosis caused by severe DNA damage, high expression of phosphorylated H2A.x is considered as an accurate indicator of apoptosis.
References
Product Information
FormatAlexaFluor®647
PresentationPurified mouse conjugate in buffer containing PBS, 15 mg/ml BSA and 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-phospho Histone H2A.X (Ser139) Antibody, clone JBW301, Alexa Fluor® 647 is validated for use in ICC for the detection of phospho Histone H2A.X (Ser139).
Key Applications
  • Immunocytochemistry
Biological Information
ImmunogenLinear peptide corresponding to phospho Histone H2A.X (Ser139).
CloneJBW301
Concentration0.5 mg/mL
HostMouse
IsotypeIgG1
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with vertebrates based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • H2AFX
  • H2AX
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~17 kDa observed. Refer to Cat. No. 05-636 for observed molecular weight information.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in untreated and staurosporin treated HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected phospho Histone H2A.X (Ser139) in staurosporin treated HeLa cells.

Alexa Fluor® is a registered trademark of Life Technologies®.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
05-636-AF647 04053252976247

Documentation

Anti-phospho Histone H2A.X (Ser139) Antibody, clone JBW301, Alexa Fluor® 647 MSDS

Title

Safety Data Sheet (SDS) 

Anti-phospho Histone H2A.X (Ser139) Antibody, clone JBW301, Alexa Fluor® 647 Certificates of Analysis

TitleLot Number
Anti-phospho Histone H2A.X (Ser139), -2760520 2760520
Anti-phospho Histone H2A.X (Ser139), -2816716 2816716
Anti-phospho Histone H2A.X (Ser139), clone JBW301, Alexa Fluor® 647 3082499
Anti-phospho Histone H2A.X (Ser139), clone JBW301, Alexa Fluor® 647 2888554
Anti-phospho Histone H2A.X (Ser139), clone JBW301, Alexa Fluor® 647 3073304
Anti-phospho Histone H2A.X (Ser139), clone JBW301, Alexa Fluor® 647 - 2555004
Anti-phospho Histone H2A.X (Ser139), clone JBW301, Alexa Fluor® 647 - Q2430678 Q2430678
Anti-phospho Histone H2A.X (Ser139), clone JBW301, Alexa Fluor® 647 - 3211145 3211145
Anti-phospho Histone H2A.X (Ser139), clone JBW301, Alexa Fluor® 647 - 3288745 3288745
Anti-phospho Histone H2A.X (Ser139), clone JBW301, Alexa Fluor® 647 - 3462127 3462127
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Life Science Research > Antibodies and Assays > Primary Antibodies