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ABS2250-25UG Anti-phospho ULK1(Ser746)

Anti-phospho ULK1(Ser746), Cat. No. ABS2250, is a rabbit polyclonal antibody that detects ULK1 phosphorylated on serine 746 and is tested for use in Immunofluorescence, Immunoprecipitation, and Western Blotting.

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ABS2250-25UG
25 μg  
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Overview

Replacement Information
Description
Catalogue NumberABS2250-25UG
DescriptionAnti-phospho ULK1(Ser746)
Alternate Names
  • Serine/threonine-protein kinase ULK1
  • EC:2.7.11.1
  • Serine/threonine-protein kinase Unc51.1
  • Unc-51-like kinase 1
Background InformationSerine/threonine-protein kinase ULK1 (UniProt: O70405; also known as EC:2.7.11.1, Serine/threonine-protein kinase Unc51.1, Unc-51-like kinase 1) is encoded by the Ulk1 gene (Gene ID: 22241) in murine species. ULK-1 is a cytosolic serine/threonine protein kinase that is localized in cytosol and preautophagosomal structures. Under condition of starvation, it is localized to punctate structures primarily representing the isolation membrane that sequesters a portion of the cytoplasm resulting in the formation of an autophagosome. Its protein kinase domain is localized to amino acids 16-278. It is involved in autophagy in response to starvation and acts upstream of PI3-kinase (PIK3C3) to regulate the formation of autophagophores, the precursors of autophagosomes. It acts both as a downstream effector and a negative regulator of mTORC1 via interaction with Raptor. ULK1 contains multiple phosphorylation sites, and their phosphorylation status is reported to regulate canonical autophagy. Under unstimulated conditions, it is phosphorylated at serine 637 and 757 by mTOR complex that leads to its inactivation. ULK1 is activated via phosphorylation by AMPK and also acts as a negative regulator of AMPK through phosphorylation of the AMPK subunits PRKAA1, PRKAB2 and PRKAG1. Despite its similarity with ULK2 in its enzymatic domain, there are major differences in their autophagy-related interactors and their post-translational and transcriptional regulators. ULK1 undergoes phosphorylation at serine 746 by receptor interacting protein kinase 3 (RIPK3) during genotoxic stress-induced alternative autophagy and this phosphorylated form is shown to localize exclusively on the Golgi and is essential for alternative autophagy, but not canonical autophagy. (Ref.: Torii, S., et al. (2020). Nat. Commun. 11: 1754).
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in buffer containing PBS with 0.09% sodium azide
Quality LevelMQ200
Applications
ApplicationAnti-phospho ULK1(Ser746), Cat. No. ABS2250, is a rabbit polyclonal antibody that detects ULK1 phosphorylated on serine 746 and is tested for use in Immunofluorescence, Immunoprecipitation, and Western Blotting.
Key Applications
  • Western Blotting
  • Immunoprecipitation
  • Immunofluorescence
Application NotesTested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected ULK1 phosphorylated on serine 746.

Immunoprecipitation Analysis: A representative lot immunoprecipitated phospho ULK1(Ser746) in Immunoprecipitation applications (Satoru Torii, et al. (2020). Nat. Commun. 11(1):1754).

Immunofluorescence Analysis: A representative lot detected phospho ULK1(Ser746) in Immunofluorescence applications (Satoru Torii, et al. (2020). Nat. Commun. 11(1):1754).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 16 amino acids surrounding phosphoserine 746 of mouse ULK-1.
EpitopeC-terminal half
Concentration0.73 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects ULK-1 phosphorylated on serine 746.
IsotypeIgG
Species Reactivity
  • Mouse
Species Reactivity NoteMouse.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • Ulk1
Modifications
  • Phosphorylation
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~ 42 kDa observed; 112.46 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western blotting with constructs of ULK-1 phosphorylated on Serine 746.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected MBP-tagged ULK1 construct phosphorylated on serine 746, but not in non-phosphorylated construct.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABS2250-25UG 04065267027630