Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

SCT071 BioTracker BacGO Gram-positive Bacteria Dye

Live cell imaging fluorescent probe for detection of gram-posotive bacteria in vitro, in vivo and in bacterial sludge

View Products on Sigmaaldrich.com
SCT071
1 mg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberSCT071
DescriptionBioTracker BacGO Gram-positive Bacteria Dye
OverviewGram staining has been the gold standard technique for bacterial classification based on the different physical properties of the bacterial cell‐wall structure. However, Gram staining requires several steps, that is, fixation of the bacterial cell, two steps of staining with two different dyes, followed by decolorization, and washing. Notably, the decolorization step often generates false Gram determination, and the washing step can cause significant cell loss. An alternative to the Gram staining method is a fluorescence‐based dye approach for flow cytometry or fluorescence microscopy.

BioTracker BacGO Gram-positive Bacteria Dye is an orange colored dye that can be used to identify Gram-positive bacteria by flow cytometry and fluorescence imaging. BacGO dye has been tested using real samples of colony‐forming bacteria, activated bacterial sludge, and a bacterial eye infection model. To fully demonstrate the universal selectivity of BacGO dye, it was tested on 16 bacterial strains (9 Gram‐positive and 7 Gram-negative) and compared the results with Gram staining. BacGO stained all of the known Gram‐positive bacteria and the result was validated with Gram staining. The universal selectivity of BacGO was also confirmed by flow cytometry using DAPI to stain the nuclei of all the bacteria.

Spectral properties:
Excitation: 558 nm
Emission: 573 nm

References:
Kwon H-Y et al. Development of a Universal Fluorescent Probe for Gram-Positive Bacteria. Ang. Chemie Intl Edn. June 17, 2019, 58, 8426-8431.
Alternate Names
  • Live cell imaging probe
  • Gram-positive bacteria probe
References
Product Information
Detection methodFluorescence
PresentationLyophilized
Applications
ApplicationLive cell imaging fluorescent probe for detection of gram-posotive bacteria in vitro, in vivo and in bacterial sludge
Key Applications
  • Cell Based Assays
Application NotesLive cell fluorescent imaging
Biological Information
Molecular Weight485.10
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssurancePurity: ≥ 98% confirmed by HPLC. Structure confirmed by HNMR, FNMR, LC-MS and elemental analysis
Molar Mass: 485.10 g/mol
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore BioTracker BacGO Gram-positive Bacteria Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Packaging Information
Material Size1 mg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
SCT071 04061841441052