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HTH17SF-14K MILLIPLEX® Human TH17 Panel Serum-Free. Available in China Only. All other countries, please order HTH17MAG-14K.

Simultaneously analyze multiple Th17 cytokine and chemokine biomarkers with the Th17 Bead-Based Multiplex Assays using the Luminex technology, in cell culture samples.

HTH17SF-14K
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Overview

Replacement Information
Description
Catalogue NumberHTH17SF-14K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX® Human TH17 Panel Serum-Free. Available in China Only. All other countries, please order HTH17MAG-14K.
OverviewMILLIPLEX® offers the broadest selection of analytes across a wide range of disease states and species. Once the analytes of interest have been identified, you can rely on the quality that we build into each kit to produce results you can trust. Each MILLIPLEX® panel and kit includes:
• Quality controls (QCs) provided to qualify assay performance
• Comparison of standard (calibrator) and QC lots to a reference lot to ensure lot-to-lot consistency
• Detection antibody cocktails designed to yield consistent analyte profiles within panel
Background InformationCD4 T-helper cells are major players in adaptive immunity. Based on their expression profile of transcription factors and secreted cytokines, these cells are divided into the major subsets Th1, Th2, Th17 and T regulatory (Treg) cells. Th1 cells, induced by IL-12 and enhanced by IFNγ, secrete IFNγ that activates macrophages. Induced by IL-4, Th2 cells primarily produce IL-4, IL-5, IL-13, IL-25 and IL-17E, playing a role in allergy. Treg cells, induced by TGFβ, produce the anti-inflammatory cytokines IL-10 and TGFβ and function to control T-cell responses to prevent autoimmune reactions. Th17 cells, a more recently discovered subset characterized by the production of IL-17, are induced by TGFβ combined with IL-23 and IL-1β in humans, and TGFβ, IL-6 and IL-21 in mice. Activated Th17 cells secrete IL-17A, IL-17F, IL-21, IL-22 and TNFα to promote tissue inflammation.

Th17 cells are involved in the clearance of extracellular bacteria and fungi. They are abundant in the intestinal lamina propria and function as a barrier against invading pathogens. Excessive amounts of Th17 cells have been implicated in the pathogenesis of several autoimmune diseases, including multiple sclerosis, psoriasis, juvenile diabetes, rheumatoid arthritis, Crohn's disease and autoimmune uveitis. In addition Th17 cells play an important role in tumor development, progression and metastasis, potentially in both promoting and inhibiting tumor growth.

The MILLIPLEX® MAP Human TH17 Magnetic Bead Panell enables you to focus on the relevance and therapeutic potential of the modulation of CD4 T-helper cell cytokines in your research model. Coupled with the Luminex xMAP® platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of multiple analytes simultaneously, which can dramatically improve productivity.
References
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Inter-assay: < 15
  • Intra-assay:< 10
Multiplex Ordering RequirementsFor questions, contact Technical Service.
Panel TypeMAGNETIC Cytokines/Chemokines
Quality LevelMQ200
Applications
ApplicationSimultaneously analyze multiple Th17 cytokine and chemokine biomarkers with the Th17 Bead-Based Multiplex Assays using the Luminex technology, in cell culture samples.
Key Applications
  • Multiplexing
Application Notes• This is an overnight incubation assay requiring 25 µL of cell culture supernatant per well.
• This kit may be used for the analysis of all or any combination of the Th17 panel analytes in tissue/cell lysate and culture supernatant
Biological Information
Species Reactivity
  • Human
Analytes Available
  • GM-CSF
  • IFN-γ
  • IL-1β
  • IL-2
  • IL-4
  • IL-5
  • IL-6
  • IL-9
  • IL-10
  • IL-12 (p70)
  • IL-13
  • IL-15
  • IL-17A
  • IL-17F
  • IL-17E/IL-25
  • IL-21
  • IL-22
  • IL-23
  • IL-27
  • IL-28A
  • IL-31
  • IL-33
  • MIP-3α/CCL20
  • TNF-α
  • TNFβ
Cross ReactivityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Accuracy
  • 79 - 107%
Physicochemical Information
Sensitivity
  • Refer to kit protocol for sensitivities of individual analytes.
Standard Curve Range
  • IL-17 & IL-4, 24 - 100,000 pg/mL
  • GM-CSF & IL-27, 61 - 250,000 pg/mL
  • IFNγ, 10 - 40,000 pg/mL
  • IL-10, 1 - 5000 pg/mL
  • MIP-3α, IL-1β, IL-12 (p70), IL-15, IL-21 & IL-33, 5 - 20,000 pg/mL
  • IL-13, 7 - 30,000 pg/mL
  • IL-17A & IL-2, 12 - 50,000 pg/mL
  • IL-22 & TNFβ, 37 - 150,000 pg/mL
  • IL-9, 9 - 35,000 pg/mL
  • IL-23, 336 - 1,500,000 pg/mL
  • IL-5, 6 - 25,000 pg/mL
  • IL-6 & TNFα, 3 - 10,000 pg/mL
  • IL-17E/IL-25, 488 - 2,000,000
  • IL-28a & IL-31, 49 - 200,000 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
HTH17SF-14K 04065267238173

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