Qty	Catalogue Number	Ordering Description	Qty/Pack	List Price
			96-Well Plate

Qty	Catalogue Number	Ordering Description	Qty/Pack	List Price
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

MultiScreen^®_HTS-PCF Filter Plates for Solubility Assays

Increased screening throughput and efficiency More

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Cell Culture & Systems

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Overview

Specifications

Ordering Information

MultiScreen PlatesClear Sorting & Filtering Show Filter Hide Filter
Catalogue Number	Description	Pack Size
MSSLBPC10	MultiScreen_HTS-PCF Filter plate, non-sterile	10
MSSLBPC50	MultiScreen_HTS-PCF Filter plate, non-sterile	50
MSRLN0410	MultiScreen Solvinert 96 Well Filter Plate	10
MSRLN0450	MultiScreen Solvinert 96 Well Filter Plate	50
MSHVN4510	MultiScreen_HTS HV Filter Plate, 0.45 µm, clear, non-sterile	10
MSHVN4550	MultiScreen_HTS HV Filter Plate, 0.45 µm, clear, non-sterile	50
MZHVN0W10	MultiScreen_HTS 384 well	10
MZHVN0W50	MultiScreen_HTS384 well	50
MDRLN0410	MultiScreen Deep, 96 Well Solvinert	10
MSGVN2210	MultiScreen_HTS GV Filter Plate, 0.22 µm, clear, non-sterile	10
MSGVN2250	MultiScreen_HTS GV Filter Plate, 0.22 µm, clear, non-sterile	50

Required EquipmentClear Sorting & Filtering Show Filter Hide Filter
Catalogue Number	Description	Pack Size
MSCPNPS00	96-well Collection Plate	100
MSCPNUV40	96-well Collection Plate	40
MSCPNPP00	96-well Collection Plate	100

Documentation

References

Reference overview
Use of ADME screening to conserve resources in drug discovery and development Weiss, A. and Joseph Machamer American Biotechnology Laboratory, January 2004: 16-17 2004
Screening for pharma gold: Researchers are using membrane technologies for high-throughput ADME analysis Lynch, John,Modern Drug Discovery, January 2004: 27-28 Modern Drug Discovery, January 2004: 27-28 2004
Assessing High-Throughput Compound Solubility Onofrey, Thomas J.,Genetic Engineering News, Feb. 14, 2004: 34, 38 Genetic Engineering News, Feb. 14, 2004: 34, 38 2004
Screening compounds for aqueous solubility: a new automated, high throughput method for solubility determination Weiss, Alan and John Lynch PreClinica, March 2003 2003

FAQ

Question	Answer
What is the well depth and maximum volume capacity of a MultiScreen plate?	The well depth of a 96 well MultiScreen plate is 1.245 cm. The well depth for a 384 well MultiScreen plate is 1.2 cm. The maximum working volume of a 96 well plate is 300 ul. The maximum working volume for a 384 well plate is 100 ul.

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Life Science Research > Cell Culture and Systems > Cell Culture Flasks, Plates, & Slides > Multiwell Plates

C8875Increased screening throughput and efficiency

Determining a compound's aqueous solubility has become an essential early measurement to make in the drug discovery process. Poor water solubility can cause problems in many different in vitro testing techniques leading to unreliable results and/or reproducibility problems; insoluble precipitates have been shown to cause false positives in bioassays potentially wasting valuable resources. Water solubility also influences absorption and thus can be used to help predict the bioavailability of a molecule.

Protocol

1.	Add compound dissolved in organic solvent to aqueous buffer
2.	Shake for 90 minutes to allow insoluble compound to precipitate
3.	Apply vacuum to filter solution into collection plate. Precipitates remain on membrane. Analyze filtrate in collection plate to quantitate amount of compound still in solution

Performance

Results Correlate to Shake-flask

Fifteen (15) compounds were evaluated by an ASTM shake-flask method and a protocol using the MultiScreen<sub>HTS</sub>-PCF Filter plate. Shake-flask solubility was determined for solid compounds added to PBS. MultiScreen<sub>HTS</sub>-PCF Filter plate results were determined for 10 µL of 10 mM DMSO stocks added to 190 µL PBS for a final DMSO concentration of 5%. The maximum concentration on the Y axis is given as 500 µM due to the MultiScreen<sub>HTS</sub>-PCF Filter plate protocol that has an upper limit for the amount of compound added.

Fifteen (15) compounds were evaluated by an ASTM shake-flask method and a protocol using the MultiScreen_HTS-PCF Filter plate. Shake-flask solubility was determined for solid compounds added to PBS. MultiScreen_HTS-PCF Filter plate results were determined for 10 µL of 10 mM DMSO stocks added to 190 µL PBS for a final DMSO concentration of 5%. The maximum concentration on the Y axis is given as 500 µM due to the MultiScreen_HTS-PCF Filter plate protocol that has an upper limit for the amount of compound added.

High Drug Recovery

Soluble drugs were dissolved in 5% DMSO/PBS at 1 µM, incubated in the MultiScreen<sub>HTS</sub>-PCF Filter plate and filtered into a receiver plate. The results are reported as percent drug recovery as determined by radiometric analysis.

Soluble drugs were dissolved in 5% DMSO/PBS at 1 µM, incubated in the MultiScreen_HTS-PCF Filter plate and filtered into a receiver plate. The results are reported as percent drug recovery as determined by radiometric analysis.

Validated for High Drug Recovery

The MultiScreen_HTS-PCF Filter plate incorporates low-binding membrane and low-binding plate materials to yield the high drug recovery needed for solubility results. The plate is validated versus a panel of 9 drugs for >80% drug recovery at 10 µM concentration in 5% DMSO/PBS.

Increased Screening Throughput and Efficiency

The MultiScreen_HTS-PCF Filter plate is a high throughput, 96-well system to classify or quantify the aqueous solubility of compound libraries stored in solvent as a concentrated solution.

The filtration-based protocol is fast and efficient. The protocol has been validated to screen hundreds of samples per day and saves time and sample over the shake-flask method. Typical MultiScreen_HTS-PCF Filter plate analysis time is less than 4 hours and uses <100 µg of sample per analysis. This is an improvement as compared to the shake-flask method that can take several days and require milligrams of sample for each analysis.

MultiScreen_HTS-PCF Filter plates are automation compatible. The plates are in compliance with SBS guidelines for easy handling by robotics. They also include a space for barcoding.

Applications

Solubility Assays

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