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17-493 PI3 Kinase Activity/Inhibitor ELISA

This PI3 Kinase Activity/Inhibitor ELISA is used to measure & quantify PI 3-Kinase levels.

PI3 Kinase Activity/Inhibitor ELISA MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Kinase Assay
17-493
1 plate  Kit capacity: 96 wells
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Key ApplicationsAnalytes AvailableDetection Methods
KAPI 3-Kinase Colorimetric HTRF
Description
Catalogue Number17-493
DescriptionPI3 Kinase Activity/Inhibitor ELISA
References
Product Information
Components
  • Glutathione-Coated Plate, 96-well strip format
  • 20% Tween® 20
  • 20X TBS Wash
  • Substrate TMB
  • Stop Buffer
  • SA-HRP Detection Reagent
  • GRP1-GST
  • Kinase Reaction Buffer, 5X
  • Wortmannin
  • PIP2
  • Biotinylated-PIP3
  • PI3K, p110α
  • PI3K, p110β
  • PI3K, p110δ
  • PI3K, p120γ
Detection methodColorimetric HTRF
Quality LevelMQ100
Applications
ApplicationThis PI3 Kinase Activity/Inhibitor ELISA is used to measure & quantify PI 3-Kinase levels.
Key Applications
  • Kinase Assay
Biological Information
Analytes Available
  • PI 3-Kinase
Entrez Gene Number
Entrez Gene SummaryPhosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers.
Gene Symbol
  • p110-alpha
  • MGC142161
  • PI3K
  • MGC142163
  • PIK3CA
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P42336 # Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2.
SIZE: 1068 amino acids; 124284 Da
SUBUNIT: Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunit. Binds to IRS1 in nuclear extracts. Interacts with RUFY3 (By similarity).
DISEASE: SwissProt: P42336 # Defects in PIK3CA are associated with colorectal cancer (CRC) [MIM:114500]. & Defects in PIK3CA are associated with breast cancer [MIM:114480]. & Defects in PIK3CA are associated with epithelial ovarian cancer (EOC) [MIM:604370].
SIMILARITY: SwissProt: P42336 ## Belongs to the PI3/PI4-kinase family. & Contains 1 C2 domain. & Contains 1 PI3K/PI4K domain.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size1 plate
Material PackageKit capacity: 96 wells
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
17-493 04053252017193

Related Products & Applications

Accessories

Catalogue Number Description
14-781 PI3 Kinase (p110α(E545K)/p85α), Murine, 10 µg
14-782 PI 3-Kinase (p110α(E542K)/p85α), Human, 10 µg
14-783 PI3 Kinase (p110α(E545K)/p85α), 10 µg
14-785 PI3 Kinase (p110α/p85α) murine, 10 µg
14-786 PI3 Kinase (p110α/p65α) Murine, 10 µg
14-787 PI3 Kinase (p110α (H1047R)/p85α) Murine, 10 µg
14-788 PI3 Kinase (p110β/p85β) Murine, 10 µg
14-790 PI3 Kinase (p110α/p65α), 10 µg

Categories

Life Science Research > Protein Detection and Quantification > Activity Assays > Kinase Assays