Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

PC386 PhosphoDetect™ Anti-p53 (pSer¹⁵) (Ab-3) Rabbit pAb

This PhosphoDetect™ Anti-p53 (pSer¹⁵) (Ab-3) Rabbit pAb is validated for use in Immunoblotting , Immunoprecipitation for the detection of p53 (pSer¹⁵) (Ab-3).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
View Products on Sigmaaldrich.com
PC386
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, M, RRbPolyclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
PC386-50ULCN 塑膠安瓿;塑膠針藥瓶 50 ul
Description
OverviewRecognizes the ~53 kDa p53 protein phosphorylated at Ser15 in PC12 or HeLa cells treated with DNA damaging agents. Does not recognize unphosphorylated p53.
Catalogue NumberPC386
Brand Family Calbiochem®
Application Data
Detection of human p53, phophorylated on Ser15, by immunoblotting. Samples: Whole cell lysates from untreated HCT116 cells (Lane A) and HCT116 cells treated with ionizing radiation (Lane B). Primary antibody: PhosphoDetect™ Anti-p53 (pSer15) (Ab-3) Rabbit pAb (Cat. No. PC386) (1:1000). Detection: chemiluminescence.
References
ReferencesTibbetts, R.S., et al. 1999. Genes Dev. 13, 152.
Levine, A.J. 1997. Cell 88, 323.
Milczarek, G.J., et al. 1997. Life Sci. 60, 1.
Shieh, S.Y., et al. 1997. Cell 91, 325.
Meek, D.W. 1994. Semin Cancer Biol. 5, 203.
Product Information
FormLiquid
FormulationIn 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
Positive controlPC-12, HCT116, or HeLa cells treated with DNA damaging agents
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Immunoprecipitation
Application NotesImmunoblotting (1:1000)
Immunoprecipitation (1:200)
Application CommentsDoes not recognize non-phosphorylated p53. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser¹⁵ phosphorylation site of human p53, conjugated to KLH
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C). Avoid freeze/thaw cycles.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
PC386-50ULCN 04055977224559

Documentation

PhosphoDetect™ Anti-p53 (pSer¹⁵) (Ab-3) Rabbit pAb MSDS

Title

Safety Data Sheet (SDS) 

PhosphoDetect™ Anti-p53 (pSer¹⁵) (Ab-3) Rabbit pAb Certificates of Analysis

TitleLot Number
PC386

References

Reference overview
Tibbetts, R.S., et al. 1999. Genes Dev. 13, 152.
Levine, A.J. 1997. Cell 88, 323.
Milczarek, G.J., et al. 1997. Life Sci. 60, 1.
Shieh, S.Y., et al. 1997. Cell 91, 325.
Meek, D.W. 1994. Semin Cancer Biol. 5, 203.

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision28-April-2008 JSW
ApplicationImmunoblotting (1:1000)
Immunoprecipitation (1:200)
Application Data
Detection of human p53, phophorylated on Ser15, by immunoblotting. Samples: Whole cell lysates from untreated HCT116 cells (Lane A) and HCT116 cells treated with ionizing radiation (Lane B). Primary antibody: PhosphoDetect™ Anti-p53 (pSer15) (Ab-3) Rabbit pAb (Cat. No. PC386) (1:1000). Detection: chemiluminescence.
DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~53 kDa p53 protein phosphorylated on Ser15.
Backgroundp53 tumor suppressor proteins play a key role in cellular defense against DNA damaging agents. p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro. DNA damage results in the phosphorylation of p53 at Ser15, leading to the reduced interactions of p53 with its negative regulator, the oncoprotein MDM2. p53 can be phosphorylated by ATM, by ATR, and by the DNA-dependent protein kinase (DNA-PK) at Ser15. This impairs the ability of MDM2 to bind p53 and promotes both the accumulation and functional activation of p53 in response to DNA damage.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser¹⁵ phosphorylation site of human p53, conjugated to KLH
IsotypeIgG
Specieshuman, mouse, rat
Positive controlPC-12, HCT116, or HeLa cells treated with DNA damaging agents
FormLiquid
FormulationIn 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
PreservativeNone
CommentsDoes not recognize non-phosphorylated p53. Antibody should be titrated for optimal results in individual systems.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C). Avoid freeze/thaw cycles.
Toxicity Standard Handling
ReferencesTibbetts, R.S., et al. 1999. Genes Dev. 13, 152.
Levine, A.J. 1997. Cell 88, 323.
Milczarek, G.J., et al. 1997. Life Sci. 60, 1.
Shieh, S.Y., et al. 1997. Cell 91, 325.
Meek, D.W. 1994. Semin Cancer Biol. 5, 203.