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03-0159-00 SMC™ Human Interleukin 17A (IL-17A) High Sensitivity Immunoassay Kit

The sensitivity of the Single Molecule Counting (SMC™) technology gives the SMC™ Human IL-17A High Sensitivity Immunoassay Kit unparalleled performance in the quantification of IL-17A in human serum and plasma.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Single Molecule Counting (SMC®) Research Category: Inflammation & Immunology Research Sub-Category: Inflammatory Disease, Immune Response, Autoimmune disease
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03-0159-00
96 well assay  
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Overview

Replacement Information

Key Spec Table

Species ReactivityKey Applications
M, R, Eq, Ca, RbSingle Molecule Counting (SMC®)
Description
Catalogue Number03-0159-00
Trade Name
  • SMC®
DescriptionSMC™ Human Interleukin 17A (IL-17A) High Sensitivity Immunoassay Kit
OverviewThe SMC™ Human IL-17A High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The Erenna® and SMCxPRO™ immunoassay systems detect analytes using Single Molecule Counting (SMC™) technology.
Background InformationInterleukin 17A (IL-17A) is a proinflammatory cytokine produced by activated T cells and has been shown to have clinical significance in chronic inflammatory diseases such as rheumatoid arthritis, multiple sclerosis and psoriasis. In tumorigenesis, IL-17A has shown mixed effects, enhancing tumor growth in some cancers while possibly promoting the production of antitumor cytokines in others. This cytokine is an important player in numerous diseases and this role makes it a key drug target pathway in the study of diseases and clinical trials in humans.
References
Product Information
Components
  • Assay Buffer
  • IL-17A Beads
  • Standard Diluent
  • IL-17A Detection Antibody
  • IL-17A Standard
  • 10X Wash Buffer
  • Buffer D
  • Elution Buffer B
Precision, %
  • Intra-assay CV% average (n=20): <10%
  • Inter-assay CV% average (n=5): <15%
Applications
ApplicationThe sensitivity of the Single Molecule Counting (SMC™) technology gives the SMC™ Human IL-17A High Sensitivity Immunoassay Kit unparalleled performance in the quantification of IL-17A in human serum and plasma.
Key Applications
  • Single Molecule Counting (SMC®)
Application NotesThis new streamline kit has been improved with the following features while the kit performance is consistent with the legacy IL-17A kit, #03-0103-00:
• Improved options for sample prep and incubation
• Lyophilization of the standard provides the kit at one storage condition
• Removal of a plate transfer step
• Overnight incubation can be used
• Increased reagent volumes to accommodate automation
• Validated to run on both SMCxPRO™ and Erenna®
Biological Information
Species Reactivity
  • Mouse
  • Rat
  • Horse
  • Canine
  • Rabbit
Species Reactivity NoteAlthough IL-17A was detectable in mouse, rat, equine, canine, and rabbit samples, the assay has not been validated for these species.
Accuracy
  • Minimum of 5 serum & plasma samples spiked with average recovery of 89% with a range of 85-92%.
Physicochemical Information
Sensitivity
  • Lower Limit of Quantification: 0.03 pg/mL (Erenna® platform)
  • 0.07 pg/mL (SMCxPRO™ platform)
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size96 well assay
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
03-0159-00 04054839536113