Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

CL07 TMB Solution

This TMB Solution is validated for use in ELISA for the detection of TMB Solution.

TMB Solution MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
View Products on Sigmaaldrich.com
CL07
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
CL07-1000MLCN 玻璃瓶 1000 ml
CL07-100MLCN 塑膠安瓿;塑膠針藥瓶 100 ml
Description
OverviewA soluble, non-toxic, non-flammable horseradish peroxidase substrate.
Catalogue NumberCL07
Brand Family Calbiochem®
Application Data
TMB (Soluble Substrate) produces a higher signal with the lowest background and outperforms other commercially available TMB single-component substrates offering the best signal to noise ratio possible.

Different lots of TMB (Soluble Substrate) were compared in an ELISA containing 5 µg horseradish peroxidase (HRP) conjugate. The absorbance at 620 nm was measured at the indicated times.

TMB (Soluble Substrate) was stored at 0°C, 4°C, 25°C or 37°C for 30 days and then tested for stability. The following graph demonstrates the stability of TMB (Soluble Substrate) where performance and background absorbance values remain consistent even after 30 days of stress conditions.
References
Product Information
CAS number54827-17-7
FormLiquid
FormulationIn a mildly acidic buffer with proprietary stabilizers, pH 3.55 ± 0.20.
Quality LevelMQ100
Applications
Application NotesELISA
Application CommentsProduct does not contain hazardous ingredients such as polar aprotic solvents (i.e., N,N-Dimethylformamide (DMF) or DMSO) that are found in other TMB formulations. There is also no alcohol in TMB (Soluble Substrate) so it is not flammable and does not require special handling for shipping. Unreacted TMB (Soluble Substrate) should be colorless or a very light-bluish-green prior to use.
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSDV2300000
Safety Information
R PhraseR: 21/22-36/38

Harmful in contact with skin and if swallowed.
Irritating to eyes and skin.
S PhraseS: 26-36/37-45

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing and gloves.
In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Harmful
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
CL07-1000MLCN 04055977228496
CL07-100MLCN 04055977228489

Documentation

TMB Solution MSDS

Title

Safety Data Sheet (SDS) 

TMB Solution Certificates of Analysis

TitleLot Number
CL07
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision21-August-2007 RFH
ApplicationELISA
Application Data
TMB (Soluble Substrate) produces a higher signal with the lowest background and outperforms other commercially available TMB single-component substrates offering the best signal to noise ratio possible.

Different lots of TMB (Soluble Substrate) were compared in an ELISA containing 5 µg horseradish peroxidase (HRP) conjugate. The absorbance at 620 nm was measured at the indicated times.

TMB (Soluble Substrate) was stored at 0°C, 4°C, 25°C or 37°C for 30 days and then tested for stability. The following graph demonstrates the stability of TMB (Soluble Substrate) where performance and background absorbance values remain consistent even after 30 days of stress conditions.
DescriptionA soluble, non-toxic, non-flammable horseradish peroxidase substrate.
BackgroundTMB (Soluble Substrate) is a non-carcinogenic chromophore for use in ELISA and other microwell applications. TMB (Soluble Substrate) contains 3,3',5,5'-tetramethylbenzidine formulated in a mildly acidic buffer with proprietary stabilizers. It is supplied as a one component liquid solution at a ready-to-use working dilution for application use. Unreacted substrate should be colorless or a very light-bluish-green prior to use. The substrate system develops a blue reaction product when reacted with peroxidase conjugates in microwell applications for visual reading at a wavelength of 370 nm and between 620 and 650 nm. For endpoint assays, the addition of an equal volume of 1N or 2N HCl, or 1N H2SO4 stops the reaction and turns the blue reaction product to a yellow reaction product and is read at a wavelength of 450 nm. Endpoint assays should be read between thirty and sixty minutes after addition of stopping solution. TMB (Soluble Substrate) produces a soluble reaction product that is not recommended for membrane or immunohistochemical applications, which require a precipitating reaction product.
FormLiquid
FormulationIn a mildly acidic buffer with proprietary stabilizers, pH 3.55 ± 0.20.
CAS number54827-17-7
RTECSDV2300000
CommentsProduct does not contain hazardous ingredients such as polar aprotic solvents (i.e., N,N-Dimethylformamide (DMF) or DMSO) that are found in other TMB formulations. There is also no alcohol in TMB (Soluble Substrate) so it is not flammable and does not require special handling for shipping. Unreacted TMB (Soluble Substrate) should be colorless or a very light-bluish-green prior to use.
Storage Protect from light
+2°C to +8°C
Do Not Freeze Yes
Toxicity Harmful