164610 p-Aminophenylmercuric Acetate - CAS 6283-24-5 - Calbiochem

164610
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Overview

Replacement Information

Key Spec Table

Empirical FormulaCAS #
C₈H₉HgNO₂ 6283-24-5

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164610-1SETCN
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      纖維紙箱 1 set
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      164610-700MGCN
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          Plastic Bag(s) 700 mg
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          Description
          OverviewAn organomercurial agent used for the activation of latent MMPs in vitro. Acts by facilitating the loss of the enzyme propeptide domain through an autolytic cleavage known as the cysteine switch.
          Note: 1 set = 5 x 700 mg.
          Catalogue Number164610
          Brand Family Calbiochem®
          SynonymsAPMA
          References
          ReferencesChen, J.-M., et al. 1991. J. Biol. Chem. 266, 5113.
          Marcy, A.I., et al. 1991. Biochemistry 30, 6476.
          Stricklin, G.P., et al. 1983. Biochemistry 22, 61.
          Product Information
          CAS number6283-24-5
          ATP CompetitiveN
          FormYellow solid
          Hill FormulaC₈H₉HgNO₂
          Chemical formulaC₈H₉HgNO₂
          ReversibleN
          Structure formula ImageStructure formula Image
          Quality LevelMQ100
          Applications
          Biological Information
          Primary TargetActivation of latent MMPs in vitro
          Purity≥90% by titration
          Physicochemical Information
          Cell permeableN
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          RTECSOV5550000
          Safety Information
          R PhraseR: 26/27/28-36/37/38

          Very toxic by inhalation, in contact with skin and if swallowed.
          Irritating to eyes, respiratory system and skin.
          S PhraseS: 36

          Wear suitable protective clothing.
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Highly Toxic
          Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
          Storage +15°C to +30°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, refrigerate (4°C). Stock solutions are stable for up to 1 month at 4°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          p-Aminophenylmercuric Acetate - CAS 6283-24-5 - Calbiochem MSDS

          Title

          Safety Data Sheet (SDS) 

          p-Aminophenylmercuric Acetate - CAS 6283-24-5 - Calbiochem Certificates of Analysis

          TitleLot Number
          164610

          References

          Reference overview
          Chen, J.-M., et al. 1991. J. Biol. Chem. 266, 5113.
          Marcy, A.I., et al. 1991. Biochemistry 30, 6476.
          Stricklin, G.P., et al. 1983. Biochemistry 22, 61.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision06-September-2007 RFH
          SynonymsAPMA
          DescriptionAn organomercurial agent used for the activation of latent matrix metalloproteinases (MMPs) in vitro. Acts by facilitating the loss of the enzyme propeptide domain through an autolytic cleavage known as the cysteine switch.
          FormYellow solid
          Recommended reaction conditionsThe MMPs are members of a family of at least twenty Zn2+-dependent endopeptidases that function extracellularly at neutral pH. The MMPs are initially synthesized as latent or inactive zymogens that contain a regulatory propeptide. This propeptide confers the latency by binding to the Zn2+ at the enzyme's active site. MMP activation can be achieved by autoactivation and by the action of other MMPs or proteases, such as furin, urokinase, plasmin, and trypsin, as well as by organomercurial compounds. The following is a protocol from Stricklin, et al. (1983. Biochemistry 22, 61) describing the use of paminophenylmercuric acetate (APMA) to activate pro-MMPs. This protocol is good for other types of organomercurials such as p-(hydroxymercuric) benzoate (PHMB), phenylmercuric chloride (PMC), and mersalyl. Organomercurial stock solutions Prepare stock solutions in 0.1 M NaOH at a concentration range of 10-50 mM just prior to use. Although not absolutely necessary, the stock solution may be adjusted to pH 11 with 5 N HCl. (e.g. Marcy, A.I. et al. 1991. Biochemistry 30, 6476). ProMMPs Prepare proenzyme solutions in 0.1 M Tris-HCl, pH 7.5 at 1-2 mg/ml. Activation of proMMPs To initiate activation, combine proMMP solution with organomercurial stock in a 10:1 volume* ratio (MMPs to organomercurial). Incubate the mixture at 37°C for 2-3 hrs**. *When more organomercurial is desired, make the stock solution more concentrated. Do not exceed the above ratio so that the volume of organomercurial in the mixture is more than about 1/10 of that of the proMMP solution; this could result in significant changes in pH. **It is recommended that an analytical run be conducted first to determine the optimal incubation time. For example, a small-scale experiment with fixed concentration of proMMP and organomercurial would be incubated as described above. Remove aliquots of sample at various time points. Stop the reaction by the addition of concentrated SDS-sample buffer (for example, add 10 µl of 2X sample buffer to a 10 µl aliquot or add 2 µl of 5X sample buffer to an 8 µl aliquot) and then boil. The progress of the activation can then be monitored qualitatively by analyzing these time aliquots on a 12% SDS-PAGE gel. Removing organomercurial from the mixture after activation The activated MMPs can be used without the removal of the organomercurial. Researchers are referred to the following reference for the removal of organomercurial by gel filtration if desired: Marcy, A.I., et al. 1991. Biochemistry 30, 6476
          CAS number6283-24-5
          RTECSOV5550000
          Chemical formulaC₈H₉HgNO₂
          Structure formulaStructure formula
          Purity≥90% by titration
          SolubilityDMSO (10 mg/ml) or 0.1 N NaOH (10 mg/ml)
          Storage +15°C to +30°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, refrigerate (4°C). Stock solutions are stable for up to 1 month at 4°C.
          Toxicity Highly Toxic
          ReferencesChen, J.-M., et al. 1991. J. Biol. Chem. 266, 5113.
          Marcy, A.I., et al. 1991. Biochemistry 30, 6476.
          Stricklin, G.P., et al. 1983. Biochemistry 22, 61.