Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
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Select A Species, Panel Type, Kit or Sample Type
To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
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96-Well Plate
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Add Additional Reagents (Buffer and Detection Kit is required for use with MAPmates)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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This page describes common challenges encountered when lysing cells and extracting proteins prior to Western blotting. Total protein concentration must be determined for these cell lysates. Variables affecting each of these steps are outlined below, as each could affect the sensitivity and reproducibility of the Western blot.
The possible causes and potential remedies for challenges encountered during preparation of samples for SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) and optimizing electrophoresis conditions.
...and pathway assays, with relevant lysate controls included in each kit, these lysates are also recommended for use in Western blotting and immunoprecipitation. Browse our MILLIPLEX%C2%AE Cell Signaling Lysates Lysate and Treatment Catalogue Number ...
Mesenchymal stem cell lines are defined as adherent multipotent progenitor cells with the capacity to differentiate into several mesenchymal cell lineages.
Traditional protein assays are not suitable for analyzing extracellular vesicle preparations because the presence of lipids and other nonprotein components in the sample may interfere with protein analysis. Learn more about how IR-based assay-free quantitation afforded by Direct Detect system can be used to in extracellular vesicle preparation.
LC3-II enrichment Kit (Western Blot) enables quantification of autophagosome density by utilizing a permeabilization procedure that reveals quantities of LC3-II, without interference from LC3-I, by Western blotting analysis.
Extracellular matrix (ECM) proteins regulate cell adhesion, differentiation, proliferation, migration, invasion, and survival. The nature of neural architecture requires an intricate relationship with the ECM.
Our membrane protein extraction kits allow isolation of proteins from mammalian cells and tissue, and can enable extraction of transmembrane proteins such as GPCRs.