Saltar al contenido
Merck

OP33

Anti-p53 (Ab-5) (Wild type) Mouse mAb (PAb1620)

liquid, clone PAb1620, Calbiochem®

Sinónimos:

Anti-p53 Antibody, Mouse Anti-p53, p53 Detection Antibody

Iniciar sesión para ver los precios por organización y contrato.

Seleccione un Tamaño

Todas las fotos(1)

About This Item

NACRES:
NA.41
UNSPSC Code:
12352203

Nombre del producto

Anti-p53 (Ab-5) (Wild type) Mouse mAb (PAb1620), liquid, clone PAb1620, Calbiochem®

biological source

mouse

antibody form

purified antibody

antibody product type

primary antibodies

clone

PAb1620, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human, mouse, rat, primate, bovine

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

dilution

(Frozen Sections (see applications references)
Immunoblotting (not recommended)
Immunofluorescence (1-20 μg/mL)
Immunoprecipitation (1 μg per sample)
Paraffin Sections (5 μg/mL, heat pre-treatment required))

isotype

IgG2a

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... TP53(7157)

Analysis Note

Positive Control
Hs27 cells or breast carcinoma tissue

Application

Frozen Sections (see applications references)

Immunoblotting (not recommended)

Immunofluorescence (1-20 μg/ml)

Immunoprecipitation (1 μg per sample)

Paraffin Sections (5 μg/ml, heat pre-treatment required)

Disclaimer

Toxicity: Standard Handling (A)

General description

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0 Ag14 myeloma cells. Recognizes the ~53 kDa wild-type p53 protein.
Recognizes the ~53 kDa wild-type p53 protein in its native conformation in Hs27 cells and breast carcinoma tissue. Does not recognize mutant or denatured p53 protein.
This Anti-p53 (Ab-5) (Wild type) Mouse mAb (PAb1620) is validated for use in Frozen Sections, Immunoblotting, IF, IP, Paraffin Sections for the detection of p53 (Ab-5) (Wild type).

Immunogen

vLM mouse tumor cells

Other Notes

El-Deiry, W.S., et al. 1994. Cancer Res.54, 1169.
Greenblatt, M.S., et al. 1994. Cancer Res.54, 4855.
Barak, Y., et al. 1993. EMBO J.12, 461.
Kastan, M.B., et al. 1992. Cell71, 587.
Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA89, 7491.
Lane, D.P. 1992. Nature358, 15.
Kastan, M.B., et al. 1991. Cancer Res.51, 6304.
Ball, R.K., et al. 1984. EMBO J.3, 1485.
Wild-type p53 has a short half-life and is present in low amounts in cells. Increasing the amount of sample to be immunoprecipitated and applied to the gel may help for visualization. Short incubation times with 35S-Met(≤ 1 hr) will help reduce background. p53 (Ab-5) may also be used to visualize p53 in cytospins or cultured cells using standard horseradish peroxidase or immunofluorescence detection techniques. p53 (Ab-5) preferentially immunoprecipitates wild-type p53; it should not precipitate mutant or denatured p53. Antibody should be titrated for optimal results in individual systems.

Packaging

Please refer to vial label for lot-specific concentration.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

¿No encuentra el producto adecuado?  

Pruebe nuestro Herramienta de selección de productos.

Clase de almacenamiento

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Hyun-Lim Kim et al.
Molecules and cells, 38(4), 312-317 (2015-03-31)
Depletion of intracellular zinc by N,N,N',N'-tetrakis(2-pyridylmethyl) ethylenediamine (TPEN) induces p53-mediated protein synthesis-dependent apoptosis of mouse cortical neurons. Here, we examined the requirement for poly(ADP-ribose) polymerase (PARP)-1 as an upstream regulator of p53 in zinc depletion-induced neuronal apoptosis. First, we found
Maria L Gomez et al.
Oncogene, 38(29), 5751-5765 (2019-06-22)
We previously reported that the dismutase SOD1 is overexpressed in breast cancer. However, whether SOD1 plays an active role in tumor formation in vivo has never been demonstrated. Further, as luminal cells of normal breast epithelial cells are enriched in
Yasuhiro Hama et al.
Journal of pharmacological sciences, 110(4), 493-496 (2009-08-05)
We have previously demonstrated an important role of influx of Cl(-) rather than Ca(2+) in acute excitotoxicity in adult rat retina. As p53 has been implicated in delayed apoptotic cell death, here we examined the appearance of p53 immunoreactivity in
Precise pancreatic cancer therapy through targeted degradation of mutant p53 protein by cerium oxide nanoparticles.
Zhang, et al.
Journal of Nanobiotechnology, 21, 117-117 (2023)
Pavel Kramata et al.
Cancer research, 65(9), 3577-3585 (2005-05-04)
Treatment of SKH-1 hairless mice with UVB (30 mJ/cm(2)) twice a week for 20 weeks results in the formation of cellular patches, long before the appearance of tumors, that are visualized in epidermal sheets with an antibody (PAb240) recognizing mutated
Ver todos los artículos relacionados

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico