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Número CAS:
UNSPSC Code:
41106310
eCl@ss:
32160414
EC Number:
293-507-3
NACRES:
NA.52
MDL number:
Biological source:
bovine (calf) thymus
Form:
lyophilized powder
Storage temp.:
−20°C
Servicio técnico
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Permítanos ayudarlebiological source
bovine (calf) thymus
Quality Segment
grade
Molecular Biology
form
lyophilized powder
mol wt
~50 kb
composition
ssDNA, ≥65%
storage temp.
−20°C
General description
High quality single-stranded template DNA isolated from the thymus of male and female calves.
Application
Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations.
Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. It was used as a standard for DNA quantification by fluorescent assay and in DNA-binding assay.
Features and Benefits
• Activated
• No nuclease activity detected after 16 h at 37 °C incubation
• High quality template DNA
• No nuclease activity detected after 16 h at 37 °C incubation
• High quality template DNA
Preparation Note
Dissolve the DNA gently rocking overnight to a final concentration of 1mg/ml in molecular biology grade water.
One mg DNA is approx. 25 A260 units.
Prepared by a modification of the method of Alberts and Herrick from calf thymus DNA.
Clase de almacenamiento
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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