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Merck

The C-terminal region of NELL1 mediates osteoblastic cell adhesion through integrin α3β1.

FEBS letters (2012-06-26)
Ai Hasebe, Yoko Nakamura, Hiroki Tashima, Kaneyoshi Takahashi, Masumi Iijima, Nobuo Yoshimoto, Kang Ting, Shun'ichi Kuroda, Tomoaki Niimi
RESUMEN

NELL1 is a secretory osteogenic protein containing several structural motifs that suggest that it functions as an extracellular matrix component. To determine the mechanisms underlying NELL1-induced osteoblast differentiation, we examined the cell-adhesive activity of NELL1 using a series of recombinant NELL1 proteins. We demonstrated that NELL1 promoted osteoblastic cell adhesion through at least three cell-binding domains located in the C-terminal region of NELL1. Adhesion of cells to NELL1 was strongly inhibited by function-blocking antibodies against integrin α3 and β1 subunits, suggesting that osteoblastic cells adhered to NELL1 through integrin α3β1. Further, focal adhesion kinase activation is involved in NELL1 signaling.

MATERIALES
Product Number
Marca
Descripción del producto

Sigma-Aldrich
Anti-Integrin β1 Antibody, clone 6S6, clone 6S6, Chemicon®, from mouse
Sigma-Aldrich
Anti-Integrin β3 Antibody, clone 25E11, clone 25E11, Chemicon®, from mouse