- Identification of immunogenic LY6K long peptide encompassing both CD4
Identification of immunogenic LY6K long peptide encompassing both CD4
Oncoimmunology (2014-10-24)
Yusuke Tomita, Akira Yuno, Hirotake Tsukamoto, Satoru Senju, Yasuhiro Kuroda, Masatoshi Hirayama, Yuya Imamura, Junji Yatsuda, Mohammad Abu Sayem, Atsushi Irie, Akinobu Hamada, Hirofumi Jono, Koji Yoshida, Takuya Tsunoda, Yataro Daigo, Hirotsugu Kohrogi, Yoshihiro Yoshitake, Yusuke Nakamura, Masanori Shinohara, Yasuharu Nishimura
PMID25340007
RESUMEN
Identification of peptides that activate both tumor-specific helper T (Th) cells and cytotoxic T lymphocytes (CTLs) are important for the induction of effective antitumor immune responses. We focused on a long peptide (LP) derived from lymphocyte antigen 6 complex locus K (LY6K) encompassing both candidate Th epitopes and a known CTL epitope. Using IFNγ ELISPOT assays as a marker of activated T cells, we studied the immunogenicity and cross-priming potential of LY6K-LP, assaying human immune cell responses in vitro and immunologic activities in HLA-A24 transgenic mice in vivo. We identified LY6K
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