USP HPLC Columns

Ascentis^® Express HILIC: Based on bare 2, 2.7, and 5 μm silica particles having 90 Å pores, these columns offer enhanced separation of polar compounds and are suitable for both HILIC and normal-phase mode. In HILIC mode, the columns offer dual separation mechanisms— ion-exchange and partition.

• Ascentis^® Si: Utilizing unbonded 3, 5, and 10 μm silica particles having 100 Å pores, these classic Si columns are for normal phase HPLC. The rigid structure of the silica surface, as opposed to the flexible nature of bonded phases, allows it to distinguish between molecules with different footprints that may have the same hydrophobicity. They provide a high-loading capacity and operates in both normal-phase and HILIC modes.
• Purospher^® STAR Si: Based on unbonded 5 μm silica particles having 120 Å pores, these columns enable separation of polar compounds using normal-phase or HILIC chromatography. They provide very high separation efficiency, as indicated by high plate counts, and feature the absence of metal impurities, ensuring consistently symmetrical peaks and extended column lifetime.

• LiChrosorb^® Si 60: Made from irregularly shaped unbonded 5 and 10 μm silica particles with a pore diameter of 60 Å, these polar sorbents are designed for use in normal-phase HPLC applications.
• LiChrospher^® Si: Based on traditionally produced unbonded 5 and 10 μm spherical silica particles, they are available in pore diameters of 60 and 100 Å. They are designed for normal-phase HPLC chromatography separations.
• SUPELCOSIL^™ LC-Si: These columns feature unmodified spherical 3 and 5 μm silica particles with a pore diameter of 120 Å. In normal-phase mode, non-polar compounds elute first, followed by polar compounds. These columns are suitable for use in both normal-phase and HILIC modes. Typical mobile phases used consist of hydrocarbon solvents such as hexane or heptane, mixed with a relatively small amount of polar solvent.
• Superspher^® Si 60: Based on unbonded 4 μm spherical silica particles with pore size of 60 Å, the sorbent is designed for normal-phase HPLC.

• Chromolith^® Performance Si: Featuring non-endcapped unbonded monolithic silica with macropores of diameter 2 μm and mesopores of size 130 Å, these non-endcapped columns offer an efficiency exceeding 80,000 plates/meter.
• Chromolith^® Prep Si: These preparative columns feature non-endcapped bare silica with 3 μm macropores and 12 nm micropores, making them ideal for higher sample loading volumes. They deliver enhanced sample throughput and faster separation, ensuring optimal productivity in preparative chromatography workflows.
• Chromolith^® SemiPrep Si: These unbonded monolithic silica columns, featuring 2 μm macropores and 13 nm micropores, are specifically designed for scale-up from analytical to semipreparative applications. These non-endcapped columns provide faster sample throughput while maintaining lower operating pressure, dramatically reducing separation time while increasing efficiency.

Ascentis^® Express C8: Featuring endcapped dimethyloctyl modified 2, 2.7, and 5 µm silica particles having 90 Å pores, the columns are designed for the enhanced retention of less hydrophobic compounds or for achieving faster separations when retention on C18 is excessive.

• Purospher^® STAR RP-8e: They are based on endcapped octylsilane-modified 2, 3, and 5 µm silica particles having 120 Å pores. The columns offer optimal performance for less hydrophobic compounds and faster retention of very hydrophobic compounds. These columns ensure excellent peak symmetry for acidic, basic, and chelating compounds. Additionally, the columns deliver enhanced selectivity for positional isomer.
• Ascentis^® C8: Made from endcapped octyl-modified 3, 5, and 10 µm silica particles having 100 Å pores. It is suitable for any method that specifies a C8-type column. The shorter alkyl chains sometimes show different selectivity toward polar compounds because they can solvate differently with the mobile phase and interact differently due to the size and shape of certain molecules. Also, C8 reagents are smaller than C18 reagents and have improved primary phase coverage, thereby requiring less endcapping. They offer excellent peak shape and very high phase stability.
• Discovery^® C8: Based on endcapped octylsilane-modified 5 µm silica particles having 100 Å pores, they offer reduced hydrophobic reversed-phase selectivity and retention while ensuring excellent peak shapes for all compounds. They exhibit very high stability and no-bleed properties, making them well-suited for LC-MS applications.
• Discovery^® BIO Wide Pore C8: Featuring octylsilane-modified 5 and 10 µm silica particles having 300 Å pores, these endcapped columns are specifically designed for reversed-phase separations, making them ideal for peptide mapping and proteolytic digest analyses.

• LiChrosorb^® RP-8: Based on totally porous, 5 and 10 µm irregular silica particles having 100 Å pores and modified with non-polar octyl group. The non-endcapped columns are well suited for the separation of neutral, acidic and weak basic compounds.
• LiChrospher^® RP-8: Made from traditionally produced 5 and 10 µm spherical silica particles having 100 Å pores. The non-endcapped columns are well suited for the separation of neutral, acidic, and weak basic compounds.
• LiChrospher^® RP-8e: The endcapped columns consist of traditionally produced 5 and 10 µm spherical silica particles having 100 Å pores. They are well suited for the separation of neutral, acidic and weak basic compounds.
• LiChrospher^® RP-Select B: It consists of traditionally produced 5 and 10 µm spherical silica particles having 60 Å pores. The octylsilane modification is deactivated for an optimized separation of basic compounds offering symmetrical peaks. These non-endcapped columns are also suitable for the determination of neutral and acidic substances.
• SUPELCOSIL™ LC-8: Made from octyl-modified 3 and 5 µm silica particles with 120 Å pores, these endcapped columns exhibit lower retention for both polar and non-polar compounds compared to C18 columns. They are designed for use with mobile phases containing 5% less organic modifier than typically required for C18 columns. Notably, polar compounds are relatively more strongly retained on C8 columns than on C18 columns.
• SUPELCOSIL™ LC-8-DB: Made from octyl-modified 3 and 5 µm silica particles with 120 Å pores. The C8-DB phase is specially deactivated for basic compounds and provides less retention, better peak shape, and higher efficiency for organic bases than can be obtained on conventional reversed-phase columns.
• Superspher^® RP-8e: Consisting of 4 µm silica particles with 60 Å pores, these octylsilane-modified endcapped columns are well suited for the separation of neutral, acidic, and weakly basic compounds.
• Superspher^® RP-8: Made of octylsilane-modified 4 µm silica particles with 60 Å pores, the non-endcapped columns are suitable for separating neutral, acidic, and weak basic compounds.
• Superspher^® RP-Select B: Constructed from 4 µm silica particles with 60 Å pores, consisting of non-polar octylsilane modification that is deactivated for the separation of basic compounds.

• Chromolith^® CapRod^® RP-8e: Made from high-purity endcapped octylsilane derivatized monolithic silica, these capillary columns feature macropores of size 2 μm and can be operated at significantly higher flow rates without loss of performance. They combine the speed of monolithic silica technology with the sensitivity required for nano-LC. They are ideal for the separation of proteins, peptides, as well as for micro-ESI, and nano-ESI applications.
• Chromolith^® HighResolution RP-8e: Consisting of octylsilane-modified monolithic slica, the non-endcapped columns feature 1.15 μm macropores and 150 Å micropores.
• Chromolith^® Performance RP-8e: These columns feature macropores with a 2 μm diameter, providing exceptional column efficiency exceeding 80,000 plates/meter. The mesopores are 13 nm (130 Å) in size, and the surface is modified with octylsilane, fully end-capped, ensuring reliable performance and enhanced separation efficiency.
• Chromolith^® WP 300 RP-8: These columns have macropores with 2 μm diameter, giving a column efficiency exceeding 80,000 plates/meter. The mesopores are 30 nm (300 Å), and the surface modification is Octylsilane.

• Purospher^® STAR NH2: They feature amino modified 5 μm silica particles having 120 Å pores. These non-endcapped column are suitable for the separation of carbohydrates and polar compounds using normal-phase or HILIC chromatography. They deliver very high separation efficiency, as evidenced by high plate counts, and ensure consistently symmetrical peaks due to the absence of metal impurities. Additionally, these columns offer an extended operational lifetime.

• LiChrospher^® NH2: Utilizing amino-modified 5 and 10 μm silica particles having 100 Å pores, the non-endcapped sorbent possesses both polar and hydrophobic properties. This makes it suitable for normal-phase, reversed-phase and ion exchange chromatography. Typical applications include the separation of carbohydrates (mono-, di- and oligosaccharides) with reversed-phase chromatography, or the separation of nucleotides by ion exchange chromatography.
• SUPELCOSIL^™ LC-NH2: Possessing 3 and 5 μm particles having 120 Å pores, the amino-modified silica column is most often employed for the separation of mono- and disaccharides.

• Chromolith^® NH2: These non-endcapped columns feature aminopropyl-modified monolithic silica with 2 μm macropores, achieving a column efficiency exceeding 80,000 plates per meter. The mesopores have a size of 130 Å, optimizing performance for high-efficiency separations.

• TSKgel^® SP-2SW: These columns are packed with 5 μm spherical silica beads featuring 125 Å pore size, surface-modified with a strong cation exchanger. They are specifically designed for the separation and analysis of small proteins, peptides, nucleosides, and other biologically active molecules.

• SUPELCOSIL™ LC-SCX: These cation-exchange columns are functionalized with strongly acidic propylsulfonic acid groups, making them ideal for the separation of cations. Retention and selectivity can be adjusted by modifying the pH, ionic strength, or organic modifier concentration. The columns are packed with 5 μm silica particles with a pore size of 120 Å.

• Ascentis^® Express ES-Cyano: Available with 2, 2.7, and 5 μm silica particles featuring 90 Å pores. The diisopropylcyanopropyl-modified, endcapped column provides enhanced retention for polar compounds and reduced retention for hydrophobic compounds. These columns are ideal for separating non-polar bases in HILIC mode using an ion-exchange mechanism. They are compatible with 100% aqueous mobile phases and exhibit stability at low pH and high temperatures.
• BIOshell^™ Peptide CN: Featuring 2.7 and 5 μm silica particles with 160 Å pores, the columns provide an alternative selectivity to the traditional C18 bonded phases. The endcapped columns are designed for the separation of peptides and small proteins, utilizing a combination of hydrophobic and dipole-dipole interactions. This makes them ideal for biomolecules that are challenging to resolve on conventional C18 columns. The optimized peptide diffusion properties enhance their suitability for peptide mapping applications. Additionally, the sterically-protected cyanopropyl ligands provide extra stability, enabling operation across an extended pH range (2–9) and at high temperatures (up to 90 °C)

• Ascentis^® ES Cyano: These endcapped columns are available with 3 and 5 μm silica particles featuring 100 Å pores. These columns provide versatile selectivity in reversed-phase mode, including for compounds with π-π and dipole-dipole interactions. Additionally, they are suitable for use in HILIC mode and normal-phase chromatography.
• Discovery^® Cyano: Cyanopropyl-modified reversed-phase columns with lower hydrophobicity than C18 or C8, that provides them with unique selectivity. They deliver excellent peak shapes and significantly less retention compared to C18, typically requiring a lower percentage of organic modifier in the mobile phase. These endcapped columns exhibit high stability and low-bleed characteristics, making them suitable for LC/MS separations. They are available with 5 μm silica particles featuring 180 Å pores.

• LiChrospher^® CN: The amino-modified non-endcapped columns feature 5 and 10 μm silica particles with 100 Å pores. They combine polar and hydrophobic properties, offering weak hydrophobic interactions alongside polar interactions, making them effective for the HPLC separation of complex samples. These columns can serve as a less polar alternative to LiChrospher® Si 60 in normal-phase applications or as a less hydrophobic alternative to LiChrospher® RP-8 in reversed-phase applications. Additionally, their versatile sorbent is suitable for selective charged interactions.
• SUPELCOSIL™ LC-CN: These columns consist of endcapped amino-modified 3 and 5 μm silica particles with 120 Å pores. Typically operated under reversed-phase conditions, they are often used as a substitute for silica due to the advantages of a bonded phase, including quick equilibration and reduced sensitivity to small variations in the aqueous content of the mobile phase.

• Chromolith^® CN: These amino-modified monolithic silica columns feature 2 μm macropores, delivering column efficiency exceeding 80,000 plates per meter. They are non-endcapped and include 130 Å mesopores, suitable for LC-MS applications. Their column efficiency is comparable to a 5 μm particle size fully porous particulate column.

• Ascentis^® Express Phenyl-Hexyl: These columns featureg 2, 2.7, and 5 μm silica particles with 90 Å or 160 Å pores that have dimethylphenyl-hexyl bonding chemistry with endcapping. They provide enhanced selectivity for aromatic compounds due to strong π-π donor interactions, making them ideal for the separation of ketones, nitriles, and alkenes. The columns are compatible with 100% aqueous mobile phases.
• BIOshell^™ IgG Diphenyl: These columns feature 2.7 μm silica particles with 1000 Å pores and a densely bonded, extensively endcapped diphenylmethyl phase. They provide stable reversed-phase packing, making them suitable for the separation of high molecular weight compounds, such as proteins.

• Ascentis^® Phenyl: These columns are available in 3, 5, and 10 μm particle sizes with 1000 Å pores and feature an endcapped phenyl ring with a short butyl spacer as the bonded phase. Utilizing a trifunctional bonding reagent, they exhibit low-bleed performance, making them suitable for MS or UV gradient applications. The high phase loading and short butyl spacer provide exceptional phenyl selectivity, and their compatibility with 100% aqueous mobile phases makes them ideal for analyzing highly polar compounds.
• Purospher^® STAR Phenyl: Available in 2, 3, and 5 μm particle sizes with 120 Å pores, they have an endcapped phenyl as the bonded phase. They offer enhanced selectivity for aromatic compounds due to strong π-π interactions, combined with low silanol activity. With excellent pH stability ranging from 1.5 to 10.5 and compatibility with up to 100% aqueous mobile phases, they are intended to be used in the separation of antibiotics, aromatic amines, flavonoids, ketones, nucleosides, PAHs, peptides, preservatives, steroids, metabolized steroids, and sweeteners.

• SUPELCOSIL™ LC-DP Diphenyl: Available in 5 μm particle size with 120 Å pores, they have an endcapped phenyl as the bonded phase. Their diphenyl bonded phase, gives them greater selectivity for aromatic groups compared to alkyl-type bonded phases.

• TSKgel^® QAE-2SW: These columns are packed with 5 µm porous spherical silica beads with 120 Å pores, surface-modified with a strong anion exchange group (trimethylamino). They are designed for the separation and analysis of peptides, nucleotides, nucleosides, and other biologically active molecules. The column is particularly suited for analyzing smaller molecular mass samples, including nucleotides, drug candidates, catecholamines, and small peptides or proteins.

• SUPELCOSIL^™ SAX1: These columns are available with a 5 μm particle size and 120 Å pores, featuring propyltrimethylammonium as the bonded phase. Is typically employed as an anion exchange column with strongly basic quaternary aminopropyl phase and is used for separating anions.

• Proteomix^® WCX-NP10: These columns are packed with spherical, non-porous poly(styrene-divinylbenzene) (PS/DVB) 10 μm particles encapsulated in a hydrophilic, neutral polymer layer to eliminate non-specific binding. A weak cation exchange group attached to the hydrophilic bonded phase, creates a high-capacity ion-exchange layer for efficient separations. They are suitable for the analysis or small scale purification of positively charged biopolymers including glycoprotein isoforms and mAb variants.
• SUPELCOGEL^™ C-610H: Comprised of 9 µm sulfonated polystyrene/divinylbenzene spherical particles, crosslinked at 6%, the non-endcapped ligand exchange column is specifically for the analysis of organic acids. They are also used in the separation of acetic, propionic, butyric, formic, malic, citric, succinic, lactic, and other acids using a simple isocratic mobile phase and minimal sample preparation.
• SUPELCOGEL^™ H: Comprised of 9 µm sulfonated polystyrene/divinylbenzene spherical particles, crosslinked at 6%, the non-endcapped ligand exchange column is specifically for the separation of carbohydrates and organic acids.

• Proteomix^® SCX-NP5: These columns are packed with spherical, non-porous poly(styrene-divinylbenzene) (PS/DVB) 5 μm particles encapsulated in a hydrophilic, neutral polymer layer to eliminate non-specific binding. A strong cation exchange sulfonic acid group is attached to the hydrophilic bonded phase using proprietary coupling chemistry, creating a high-capacity ion-exchange layer for efficient separations. They are suitable for the analysis or small scale purification of positively charged biopolymers including glycoprotein isoforms and mAb variants.
• Proteomix^® SCX-NP10: These columns include spherical, non-porous poly(styrene-divinylbenzene) (PS/DVB) particles of 10 μm diameter, enveloped in a hydrophilic, neutral polymer layer to prevent non-specific binding. A cation exchange sulfonic acid group is fixed to the hydrophilic bonded phase, establishing a high-capacity ion-exchange layer for effective separations. They are appropriate for the investigation or small-scale purification of positively charged biopolymers, including glycoprotein isoforms and monoclonal antibody variations.
• SUPELCOGEL^™ Ca: Comprised of 9 µm sulfonated polystyrene/divinylbenzene spherical particles, crosslinked at 6%, this non-endcapped ligand exchange column is intended for the separation of carbohydrates and organic acids.

• TSKgel^® QC-PAK GFC: These columns are packed with 5 µm porous spherical silica beads featuring 120 Å pores and a diol-modified phase. The TSKgel^® QC-PAK GFC 200 column is optimized for the separation of small proteins and peptides with diol functional groups, with a fractionation range suitable for globular proteins up to 150,000 Da. The TSKgel^® QC-PAK GFC 300 column is ideal for the analysis of samples with unknown molecular mass.
• TSKgel^® SuperSW: These columns feature 4 μm particles functionalized with diol groups, delivering high-efficiency separations of peptides and proteins. With a reduced internal diameter of 4.6 mm, they provide enhanced sensitivity while minimizing solvent consumption. The TSKgel® SuperSW2000 column, with a 12.5 nm (125 Å) pore size, is ideal for separating globular proteins up to 150,000 Da. The TSKgel® SuperSW3000 column, featuring a 25 nm (250 Å) pore size, accommodates globular proteins up to 500,000 Da.
• TSKgel^® SW: Based on highly porous silica particles, whose surfaces are derivatized with diol functional group-containing ligands to prevent protein interactions. These columns distinguish themselves from other silica- or polymer-based size exclusion columns by their large pore volumes. They are specifically designed for the aqueous analysis and isolation of proteins, antibodies, enzymes, nucleic acids, and other biological macromolecules.
• TSKgel^® SW_XL: Equipped with smaller particles (5 and 8 μm) compared to the TSKgel^® SW series (10 and 13 μm), they feature highly porous silica particles, whose surfaces are derivatized with diol-functionalized ligands to minimize protein interactions. These columns are widely used for quality control in biopharmaceutical applications, particularly for the analysis of monoclonal antibodies and other biologics.

• LiChrospher^® Diol: These non-endcapped columns are available in 5 and 10 μm particle sizes and 100 Å pores, featuring a diol-bonded phase. Commonly employed as an anion exchange column with a strongly basic quaternary aminopropyl phase, they are designed for the separation of anions. These columns exhibit both polar and hydrophobic properties, combining weak hydrophobic interactions with polar interactions, enabling the effective separation of complex samples. They serve as a less polar alternative to LiChrospher^® Si 60 in normal-phase applications or as a less hydrophobic alternative (with some limitations) to LiChrospher^® RP-8 in reversed-phase applications.
• SUPELCOSIL^™ LC-Diol: These columns are non-endcapped and available in 5 μm particle size with 120 Å pores, featuring a diol-bonded phase. These columns are commonly used for protein separations by gel filtration chromatography. When operated with an aqueous buffer, the diol phase effectively shields the silica surface from interacting with proteins. Under normal-phase conditions, these columns are also well-suited for the separation of steroids and sterols.

• Chromolith^® Diol: Featuring non-endcapped diol-modified monolithic silica, these columns have macropores of 2 μm diameter, giving a column efficiency exceeding 80,000 plates/meter. The mesopores are 13 nm (130 Å) in size. 

• Hamilton^® PRP-1: Constructed entirely from polystyrene-divinylbenzene, these columns offer inherent reversed-phase separation properties without the need for a stationary phase coating. The absence of silanol groups ensures exceptional sample recovery (≥ 95%). These columns are available in four particle sizes (5, 7, 10, and 12–20 µm) with a 100 Å pore size. Their stability across a wide pH range (1 to 13) enables the analysis of samples at high pH (8 to 13) without risking column degradation or loss of efficiency. The extended pH range also allows for chromatographic analysis of ionized samples.
• Hamilton^® PRP-3: Featuring a larger 300 Å pore size, these columns are polymeric reversed-phase columns specifically designed for the purification and isolation of proteins and peptides, offering excellent recovery (> 90%). The inert polymeric packing, made from poly(styrene-divinylbenzene), eliminates silanol groups, reducing the risk of irreversible protein adsorption and enhancing protein recovery. The robust chemical stability of the PRP-3 columns allows for the use of a wide range of dissolution agents, including concentrated acids, aggressive chaotropes, and detergents.
• TSKgel^® SuperH: Packed with rigid, porous polystyrene-divinylbenzene (PS-DVB) beads, this column line consists of eight columns with different pore sizes, TSKgel^® SuperH1000 through TSKgel^® SuperH7000, and four columns with an extended linear range of the calibration curve.
• TSKgel^® SuperHZ: This column line consists of five columns with different pore sizes that have been developed for high-throughput, high-efficiency GPC applications.
• TSKgel^® H_HR: These are conventional GPC columns and consist of eight columns with different pore sizes. They have a maximum operating temperature range of 140 ºC.
• TSKgel^® H_XL: These are conventional GPC columns, available as eight column types with different pore sizes. They utilize 3 µm porous spherical PS-DVB particles and have a maximum operating temperature range of 140 ºC.

• Hamilton^® PRP-X200: These columns enable rapid resolution of alkali metals, alkaline earth metals, and ammonium ions. A unique feature of the PRP-X200 column is the ability to adjust the resolution between specific ions in the alkali metal series by varying the methanol concentration in the mobile phase. This flexibility allows analysts to target specific ions of interest while minimizing interference from other ions in complex samples.
• Hamilton^® PRP-X300: These columns provide a fast and efficient method for separating closely related alcohols and organic acids. Utilizing a sulfonated poly(styrene-divinylbenzene) support, the separation occurs through a mixed-mode mechanism. Column selectivity can be fine-tuned by adjusting the pH of the buffer or incorporating organic modifiers like methanol or acetonitrile. The support's stability with organic solvents enables the analysis of samples that are excessively retained on traditional ion-exclusion supports. High-performance packing ensures narrow, sharp peaks under isocratic conditions, eliminating the need for column re-equilibration between runs. Additionally, minimal sample preparation is required, enhancing throughput and operational efficiency in the lab.
• SUPELCOGEL^™ H: Comprised of 9 µm sulfonated polystyrene/divinylbenzene spherical particles, crosslinked at 6%, the non-endcapped ligand exchange column is specifically for the separation of carbohydrates and organic acids.

• TSKgel^® G2500PWXL: Featuring 7 µm particles with a 125 Å pore size, these columns are optimized for the analysis of water-soluble linear polymers with molecular weights up to 3,000 Da. The hydroxylated methacrylate phase ensures excellent compatibility with aqueous mobile phases, enabling efficient and reliable separations.
• TSKgel^® G2500PW: These columns feature spherical, hydrophilic polymethacrylate beads with particle sizes of 12 and 17 µm and pore sizes of 125 Å. They are specifically designed for the analysis of water-soluble polymers, including celluloses, acrylamides, glycols, dextrans, polyvinyl alcohols, and oligosaccharides, with molecular weights under 3,000 Da. Stable across a broad pH range (2 to 12), these columns can be used with aqueous or buffered mobile phases containing up to 20% methanol. They are also temperature-resistant up to 80 °C, ensuring robust performance in diverse analytical conditions.
• TSKgel^® G2000PW: Featuring 12 µm particles with a 125 Å pore size composed of spherical, hydrophilic polymethacrylate beads, these columns are specifically designed for the analysis of water-soluble polymers such as celluloses, acrylamides, glycols, dextrans, polyvinyl alcohols, and oligosaccharides with molecular weights up to 2,000 Da. These columns are particularly well-suited for semi-preparative and preparative isolation of oligosaccharides. Stable across a pH range of 2 to 12, TSKgel^® G2000PW columns are compatible with aqueous or buffered mobile phases containing up to 20% methanol and can operate at temperatures up to 80 °C, ensuring versatility and durability in various analytical and preparative workflows.
• TSKgel^® G1000PW: Composed of spherical, hydrophilic polymethacrylate beads, it is the preferred column for analysis of low molecular weight oligomers.

• BIOshell™ Protein C4: These columns are designed with endcapped dimethylbutylsilane-bonded silica particles, featuring a 400 Å pore diameter and 3.4 μm particle size. Engineered for the efficient separation of larger proteins, they suitable for handling very hydrophobic proteins, including membrane proteins. The C4 ligand, combined with an endcapped surface, ensures optimal recovery of large or highly hydrophobic biomolecules. The 3.4 μm Fused-Core particles enable shorter analysis times while maintaining back pressure comparable to 1.7 μm, 300 Å fully porous C4 particles. These columns can operate at pressures up to 9000 psi (600 bar), temperatures up to 90 °C, and within a pH range of 2 to 9, ensuring durability and versatility for challenging protein separations.
• BIOshell™ IgG C4: These columns are designed with endcapped dimethylbutylsilane-bonded silica particles, featuring a 1000 Å pore diameter and 2.7 μm particle size. It provides a stable, reversed-phase packing that can be used for separating high molecular weight compounds such as proteins.

• Chromolith^® WP 300 RP-4: Based on non-endcapped octadecylsilane modified monolithic silica having 2 μm macropores and 130 Å mesopores, this hydrophobic column enables the reversed-phase separation of protein and peptides, especially complex peptide mixtures.

• Supelclean^™ LC-Si: These normal-phase columns are packed with high-purity, irregularly shaped silica gel, designed to adsorb polar analytes from nonaqueous samples. They are available in a particle size of 45 μm and a pore size of 60 Å.

• Astec^® CLC-D: The chiral column is designed for the enantiomeric separation of α-hydroxy carboxylic acids, amino acids, and other α-bifunctional compounds. It utilizes a chiral bidentate ligand in the D-form bonded to high-purity spherical 5 μm silica particles having 100 Å pores, facilitating the formation of transient diastereomeric complexes with analytes in the presence of copper(II) ions. This mechanism enables effective chiral resolution, with the L-enantiomer typically eluting before the D-enantiomer, except in specific cases like tartaric acid. The columns requires a mobile phase having 5 mM CuSO₄, and any pH adjustments can be made with acetic acid.
• Astec^® CLC-L: The chiral column is designed for the enantiomeric separation of α-hydroxy carboxylic acids, amino acids, and other α-bifunctional compounds. It utilizes a chiral bidentate ligand in the L-form bonded to high-purity spherical 5 μm silica particles having 100 Å pores, facilitating the formation of transient diastereomeric complexes with analytes in the presence of copper(II) ions. This mechanism enables effective chiral resolution, with the D-enantiomer typically eluting before the L-enantiomer, except in specific cases like tartaric acid. The columns requires a mobile phase having 5 mM CuSO₄ and any pH adjustments can be made with acetic acid.

• TSKgel^® G2000SWXL: These premium columns are intended for the analysis of biopolymers by GFC, TSKgel G2000SWxl, 5 μm columns are an excellent choice for small proteins and peptide separations. The fractionation range is applicable for globular protein samples up to 150,000 Da. These columns feature high pore volume per unit column volume, low sample adsorption and excellent column efficiency, all contributing to unsurpassed sample resolution.
• TSKgel^® G4000SWXL: These columns feature 8 μm particles with 45 nm (450 Å) pores, offering the largest exclusion limit and the widest fractionation range in the TSKgel^® SWXL series. They are specifically designed for the analysis of pegylated proteins and glycosylated biomolecules, with a fractionation range suitable for globular proteins up to 7 million Da. Known for their superior performance in gel filtration chromatography (GFC), these columns provide high pore volume, minimal sample adsorption, excellent column efficiency, and exceptional resolution, making them an ideal choice for biopolymer analysis.

• SUPELCOGEL^™ Pb: They utilize a sulfonated polystyrene/divinylbenzene resin in the lead (Pb²⁺) ionic form, facilitating effective ligand-exchange chromatography. With 9 µm spherical particles and a microporous structure optimized for monosaccharide separation, these columns are suitable for the analysis of carbohydrates such as xylose, galactose, and mannose. Designed for aqueous mobile phases, they rely on a ligand-exchange mechanism, leveraging Pb²⁺ ions for selective interactions.

• TSKgel^® G3000PWXL: Featuring 7 µm sized particles with 20 nm pores, the column is ideally suited for analysis of water-soluble linear polymers with molecular weights up to 50,000 Da. The column's base material is hydroxylated polymethacrylate, enabling high compatibility with aqueous mobile phases and up to 20% organic solvents.
• TSKgel^® G3000PW: The column is designed for the analysis of water-soluble polymers and features spherical, hydrophilic polymethacrylate beads with particle sizes of 12 and 17 µm and a pore size of 20 nm (200 Å). These columns are particularly suited for the separation of polymers such as celluloses, acrylamides, glycols, dextrans, polyvinyl alcohols, and oligosaccharides with molecular weights under 50,000 Da. Stable across a pH range of 2 to 12, the column supports mobile phases comprising water or buffers containing up to 20% methanol. With a temperature tolerance of up to 80 °C, the column ensures reliable performance for high-resolution separations in diverse analytical applications.

• TSKgel^® SuperAW: The column is packed with 4 µm rigid, porous polymer beads with a pore size of 15 nm, designed to minimize shrinkage or swelling in polar organic solvents such as methanol, acetonitrile, DMSO, isopropanol, THF, and HFIP. Stable in 100% organic solvents, this column is ideal for analyzing polymers soluble in polar organics with molecular weights up to 60,000 Da. Its compact dimensions (6 mm ID × 15 cm) reduce analysis time and solvent consumption, making it well-suited for high-throughput applications.
• TSKgel^® PW: The columns are composed of spherical, hydrophilic polymethacrylate beads with particle sizes ranging from 12 µm (smaller pore size columns) to 17 µm (larger pore size columns). They are designed for the analysis of water-soluble polymers, including celluloses, acrylamides, glycols, dextrans, polyvinyl alcohols, and oligosaccharides, offering reliable and high-resolution performance for diverse applications.
• TSKgel^® PWXL: Composed of spherical, hydrophilic polymethacrylate beads, the columns are intended for the analysis of water-soluble polymers, such as celluloses, acrylamides, glycols, dextrans, polyvinylalcohol, and oligosaccharides.

• TSKgel^® SuperAW: The column is packed with 4 µm rigid, porous polymer beads with a pore size of 15 nm, designed to minimize shrinkage or swelling in polar organic solvents such as methanol, acetonitrile, DMSO, isopropanol, THF, and HFIP. Stable in 100% organic solvents, this column is ideal for analyzing polymers soluble in polar organics with molecular weights up to 60,000 Da. Its compact dimensions (6 mm ID × 15 cm) reduce analysis time and solvent consumption, making it well-suited for high-throughput applications.
• TSKgel^® PW: The columns are composed of spherical, hydrophilic polymethacrylate beads with particle sizes ranging from 12 µm (smaller pore size columns) to 17 µm (larger pore size columns). They are designed for the analysis of water-soluble polymers, including celluloses, acrylamides, glycols, dextrans, polyvinyl alcohols, and oligosaccharides, offering reliable and high-resolution performance for diverse applications.
• TSKgel^® PWXL: Composed of spherical, hydrophilic polymethacrylate beads, the columns are intended for the analysis of water-soluble polymers, such as celluloses, acrylamides, glycols, dextrans, polyvinylalcohol, and oligosaccharides.

• Astec^® Cellulose^® DMP: It comprises of a chiral stationary phase (CSP) made up of spherical, high-purity 5 μm porous silica particles coated with DMPC (3,5-dimethylphenyl carbamate)-derivatized cellulose. It separates a wide range of chiral compounds under normal phase, polar organic, and SFC conditions, with high efficiency, high loading capacity, and excellent column lifetime. Performance is comparable to other DMPC-derivatized cellulose CSPs.

• CHIRALPAK^® AGP: The column utilizes α1-acid glycoprotein (AGP) as the chiral selector immobilized on spherical 5 μm silica particles. Designed for reversed-phase mode, it separates enantiomers of a wide variety of drug substances, including acids, amines, and neutral compounds. The column is compatible with pure organic solvents, elevated temperatures, and pH values ranging from 4 to 7. Enantioselectivity and retention are easily adjusted by modifying the mobile phase pH, ionic strength, and the type and concentration of the organic modifier, offering versatile performance for chiral separations.

• Ascentis^® Express F5: These columns feature an endcapped pentafluorophenylpropyl (F5) bonded phase on silica particles with a 90 Å pore size, available in particle sizes of 2, 2.7, and 5 μm. These columns offer exceptional selectivity for stereoisomers, strong π-π acceptor interactions, and enhanced retention of aromatic and electron-rich compounds. They can operate in both reversed-phase and HILIC modes. Compared to C18 phases, the F5 phase provides longer retention for basic analytes and reduced retention for hydrophobic analytes, making it ideal for a wide range of separation challenges.

• Discovery^® HS F5: The columns feature an end-capped pentafluorophenylpropyl (F5) bonded phase on silica particles with a 120 Å pore diameter. Available in 3, 5, and 10 μm particle sizes, these reversed-phase columns offer unique retention and selectivity, particularly for basic and halogenated compounds. They deliver separations distinct from traditional C18 columns, though compounds generally elute within a similar retention time window, enabling straightforward method transfer. The columns provide excellent peak shape, low-bleed performance, and stability, making them ideal for LC/MS applications.

• Astec^® CYCLOBOND^® I 2000 Series: Featuring underivatized, native β-cyclodextrin bonded to high-purity 5 μm silica particles with a 100 Å pore diameter. These columns are designed for the enantioselective separation of chiral compounds through inclusion complexation and hydrogen bonding interactions. Additionally, non-inclusion separations can be achieved in the polar organic mode, broadening their applicability to various molecule types.

• ChiraDex^®: Based on a beta-cyclodextrin covalently linked to 5 μm spherical silica particles with a 100 Å pore diameter. Available in dimensions of 4 mm length and 4 mm internal diameter, the non-endcapped columns are well suited for chiral separations of hydrocarbons, steroids, phenyl esters, aromatic amines, heterocycles with 5-membered ring to 7-membered ring.
• ChiraDex^® HR: Based on a beta-cyclodextrin covalently linked to 5 μm spherical silica particles with a 100 Å pore diameter. Available in dimensions of 250 mm length and 4 mm internal diameter, the non-endcapped columns are well suited for chiral separations of hydrocarbons, steroids, phenyl esters, aromatic amines, heterocycles with 5-membered ring to 7-membered ring.

• SUPELCOSIL^™ LC-SCX: The cation-exchange columns have strongly acidic propylsulfonic acid groups bonded to the 5 μm spherical silica particles with a pore diameter of 120 Å. Adjusting pH, ionic strength, and the concentration of the organic modifier allows precise control over retention and selectivity of cation-exchange separations.

• SRT^® SEC Series: The columns feature uniform, hydrophilic, and neutral nanometer thick films chemically bonded on the high purity and enhanced mechanical stability silica. They provide excellent column-to-column reproducibility, high stability, and negligible non-specific interactions Available in a variety of pore sizes (100, 150, 300, 500, 750, 1000, and 2000 Å) and particle sizes (3 and 5 μm), they are optimized for high-protein recovery while maintaining biological activity. Suitable for a wide range of sample types, including monoclonal antibodies, proteins, peptides, nucleic acids, oligonucleotides, virus, water-soluble polymers, hydrophobic proteins like insulin, membrane protein monoclonal antibodies derivatized with polymer branches, e.g. polypeptide, PEG.
• TSKgel^® SuperSW: These columns feature 4 μm particles functionalized with diol groups, delivering high-efficiency separations of peptides and proteins. With a reduced internal diameter of 4.6 mm, they provide enhanced sensitivity while minimizing solvent consumption. The TSKgel^® SuperSW2000 column, with a 12.5 nm (125 Å) pore size, is ideal for separating globular proteins up to 150,000 Da. The TSKgel^® SuperSW3000 column, featuring a 25 nm (250 Å) pore size, accommodates globular proteins up to 500,000 Da.
• TSKgel^® SW: Based on highly porous silica particles, whose surfaces are derivatized with diol functional group-containing ligands to prevent protein interactions. These columns distinguish themselves from other silica- or polymer-based size exclusion columns by their large pore volumes. They are specifically designed for the aqueous analysis and isolation of proteins, antibodies, enzymes, nucleic acids, and other biological macromolecules.
• TSKgel^® SW_XL: Equipped with smaller particles (5 μm and 7 μm) compared to the TSKgel^® SW series (10 and 13 μm), they feature highly porous silica particles, whose surfaces are derivatized with diol-functionalized ligands to minimize protein interactions. These columns are widely used for quality control in biopharmaceutical applications, particularly for the analysis of monoclonal antibodies and other biologics.

• Ascentis^® Express RP-Amide: These columns feature a stationary phase made up of high-purity C16-silica that includes a polar-embedded amide function attached to the silica surface. The endcapped column is available in particle sizes of 2, 2.7, and 5 μm with pores of 90 Å. They offer complementary selectivity to alkyl phases with improved peak shape for basic compounds compared to traditional EPG amide phases. Ideal for separating organic acids, phenols, catechins, alcohols, and bases, with compatibility for use in 100% aqueous mobile phases.

• Ascentis^® RP-Amide: Featuring endcapped palmitamidopropyl-modified silica particles with 100 Å pores, the column is available in 3, 5, and 10 µm particle sizes. It provides similar separation capabilities to C18 columns while overcoming limitations such as poor wettability in highly aqueous mobile phases. The amide functionality enhances retention and selectivity for compounds that interact through hydrophobic and hydrogen bonding interactions, making it particularly effective for phenols, organic acids, and other polar solutes. Strong hydrogen bonding between the amide carbonyl (H-bond acceptor) and H-bond donors like phenols and acids contributes to its superior performance. Compared to other EPG phases, the RP-Amide delivers retention comparable to C18 and C8, facilitating straightforward method transfer without requiring mobile phase adjustments.
• Discovery^® RP-Amide C16: Available in 5 µm particle size with 100 Å pores, the endcapped column consists of high-purity C16-silica that includes a polar-embedded amide function attached to the silica surface with a propyl group. It offers excellent retention and resolution for polar compounds with unique selectivity distinct from C18 columns. Provides superior peak shape and efficiency, while being less hydrophobic than C18, and is fully compatible with 100% aqueous mobile phases.

• SUPELCOSIL^™ ABZ+Plus: The columns offer high efficiency and unique selectivity for polar, nonpolar, and charged analytes. Featuring deactivated silica particles with a narrow particle size distribution, these columns ensure low back pressure and symmetric peak shapes, even for reactive compounds. The column is available in 3 and 5 µm silica particles with 120 Å pores, bonded to alkylamide phase. The bonded and endcapped ABZ+Plus phase shields unreacted silanol groups, allowing the use of low ionic strength buffers without ion-suppressing modifiers. Ideal for acids, strong bases, and zwitterions, these columns enable simple mobile phases.
• SUPELCOSIL^™ LC-ABZ: Made from alkylamide-modified 3 and 5 µm silica particles with 120 Å pores, the deactivated phase in the endcapped column provides excellent reversed-phase performance for basic compounds, as well as those that are acidic, polar neutral, and non-polar.

• Ascentis^® Express C30: Available in 2.7 μm particle size with 160 Å pores, the endcapped column has a triacontyldimethyl-modified silica particles. It offers excellent selectivity for hydrophobic, long-chain, and structurally related isomers.

• Astec^® CHIROBIOTIC^® T: They utilize teicoplanin, a macrocyclic glycopeptide antibiotic with 23 chiral centers, as the chiral selector, covalently bonded to high-purity, spherical silica gel. Available in a 5 μm particle size with 100 Å pores, they offer unique selectivity for a number of classes of molecules, specifically underivatized α, β, γ and cyclic amino acids, N-derivatized amino acids, hydroxy-carboxylic acids, acidic compounds including carboxylic acids and phenols, small peptides, neutral aromatic analytes, and cyclic aromatic and aliphatic amines.
• Astec^® CHIROBIOTIC^® T2: They make use of high-purity, spherical silica gel covalently bound to the chiral selector teicoplanin, a macrocyclic glycopeptide antibiotic with 23 chiral centers. Specifically,they provide unique selectivity for a variety of molecular classes, including underivatized α, β, γ, and cyclic amino acids, N-derivatized amino acids, hydroxy-carboxylic acids, acidic compounds such as carboxylic acids and phenols, small peptides, neutral aromatic analytes, and cyclic aromatic and aliphatic amines. It is available in a 5 μm particle size of 200 Å pores.
• Astec^® CHIROBIOTIC^® TAG: They use teicoplanin with its carbohydrate moieties removed—as the chiral selector, covalently bonded to high-purity, spherical silica particles with a 5 µm diameter with 100 Å pore size. This modification enhances their selectivity for various chiral compounds, including amino acids (α, β, γ, and cyclic), particularly sulfur-containing ones like methionine, histidine, and cysteine, as well as neutral molecules such as oxazolidinones, hydantoins, and diazepines.

• apHera™ C18: These reversed-phase columns feature a stable vinyl alcohol copolymer base with octadecyl (C18) bonded phase. This innovative bonding combines the hydrophobicity of traditional C18 phases with enhanced surface wettability, even under high organic loading conditions. With a 5 μm particle size and a 300 Å pore diameter, these columns are ideal for separating small molecules, peptides, and small proteins. They operate efficiently within a pH range of 2 to 12 and are compatible with a variety of organic solvents. The apHera™ C18 columns are particularly suited for basic substances, providing logical elution patterns and excellent peak shapes, while offering high durability across diverse analytical conditions.

• SUPLEX™ pKb-100: They feature an alkylamide-bonded phase on 5 µm spherical silica particles with a 120 Å pore size. Unlike the SUPELCOSIL^™ LC-ABZ columns, which are endcapped, these columns are not endcapped. Deactivated for basic compounds, these reversed-phase columns provide shorter retention, better peak shape, and higher efficiency for bases. Additionally, they simplify mobile phase preparation as no amine modifiers, such as triethylamine, are required to obtain excellent peak shapes.

• apHera^™ NH2: These amino columns are specialized for the separation of mono- and oligosaccharides, utilizing a covalently bonded polyamine phase on a robust polyvinyl alcohol (PVA) copolymer support. This design ensures mechanical and chemical stability, offering high column efficiency and extended longevity. The columns feature a 5 μm particle size and a 300 Å pore diameter, operating effectively across a pH range of 2 to 13.

• Ascentis^® Express OH5: The columns are available in 2, 2.7, and 5 μm particle sizes and 90 Å pore diameter, operating effectively across a pH range of 2 to 9. These non-endcapped columns are ideal for the HILIC separation of very polar compounds with a LogP value close to 0 or less than 0. They provide predominantly HILIC partitioning retention, exhibit limited anionic silanol interactions, and demonstrate minimal sensitivity to variations in ionic strength.
• BIOshell™ Glycan: They feature a penta-hydroxy bonded phase on superficially porous silica particles with a 2.7 μm particle size and 90 Å pore diameter. Designed for hydrophilic interaction liquid chromatography (HILIC), these columns are ideal for the high-resolution separation of oligosaccharides, including released and labeled glycans. They offer improved retention of polar compounds and zwitterions compared to bare silica and maintain consistent resolution even with slight changes in buffer concentration. Capable of resolving complex glycan mixtures, including isobaric glycans with different linkages, these columns provide excellent performance for detailed glycan analysis.

• Astec^® CHIROBIOTIC^® V: They utilize vancomycin, a macrocyclic glycopeptide antibiotic with 18 chiral centers, as the chiral selector covalently bonded to high-purity, spherical silica particles. The columns are available in particle sizes of 5, 10, or 16 μm, with a pore size of 100 Å. The columns are particularly effective for neutral molecules, amides, acids, esters, and amines, showing considerable enantioselectivity for these analytes. They demonstrae many of the separation characteristics of protein-based stationary phases but with exceptional stability and higher sample capacity.
• Astec^® CHIROBIOTIC^® V2: They use high-purity, spherical silica particles covalently bound to vancomycin, a macrocyclic glycopeptide antibiotic containing eighteen chiral centers, as the chiral stationary phase. The columns have a pore size of 200 Å and come in particle sizes of 5, 10, or 16 μm. The columns exhibit significant enantioselectivity for these analytes and are especially useful for neutral compounds, amides, acids, esters, and amines. They exhibit remarkable stability and a greater sample capacity while displaying many of the separation properties of protein-based stationary phases.

• Supel™ Carbon LC: Packed with porous graphitic carbon (PGC) particles, with a particle size of 2.7 µm and a pore size of 200 Å, these columns operate effectively across a pH range of 1 to 14 and at elevated temperatures up to 250 °C. The Polar Retention Effect on Graphite (PREG) enables efficient retention of polar and charged compounds without requiring HILIC conditions and allows the resolution of geometric isomers.

• SeQuant^® ZIC^®-HILIC: They feature a porous silica-based stationary phase covalently bonded to a zwitterionic sulfobetaine functional group with a charge balance of 1:1. They are available in various formats, including analytical, capillary, and semi-preparative sizes, with particle sizes of 3.5 µm or 5 µm and pore sizes of 100 Å or 200 Å. They are used in the analysis of a wide variety of polar hydrophilic compounds such as acids and bases, anions and cations, carbohydrates, metabolites, metal complexes, amino acids, peptides, and protein digests.

• SeQuant^® ZIC^®-pHILIC: Featuring a polymer-based stationary phase with covalently bonded, zwitterionic functional groups, they provide enhanced pH stability across a range of 2 to 10. These columns are the preferred choice when extreme eluent conditions are needed to overcome challenging separations of polar hydrophilic compounds. Available in 5 μm particle size, these polymer-based columns carry densely bonded, truly zwitterionic functional groups with a charge balance of 1:1. columns minimize background noise in evaporative detection techniques with high sensitivity to ions, such as charged aerosol detectors (CAD).