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MABE146 | Anti-5-methylcytosine Antibody, clone 33D3

MABE146
100 µg  
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      Replacement Information

      Ofertas especiais

      Tabela com principais espec.

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M DB, IF, Southern Blotting, IH(P), Methylated DNA Immunoprecipitation (MeDIP), FC M Purified Monoclonal Antibody
      Description
      Catalogue NumberMABE146
      ReplacesMABE527
      DescriptionAnti-5-methylcytosine Antibody, clone 33D3
      Alternate Names
      • methylcytosine
      • methylcytidine
      Background Information5-Methylcytosine is a modified base found in the DNA of higher eukaryotes. Methylation of DNA is known to be involved in the control of gene expression and is mediated by a class of enzymes known as DNA methyltransferases (DNMT’s). Appropriate cytosine methylation is essential for normal development and maintenance of mammalian cells. Alternations in DNA methylation patterns is often associated with cancers with the turmor DNA being hypomethylated compared to DNA from normal tissues.
      References
      Product Information
      FormatPurified
      Control
      • Methylated and unmethylated BRCA1
      PresentationPurified mouse monoclonal IgG1λ in buffer containing PBS without preservative.
      Applications
      ApplicationUse Anti-5-methylcytosine Antibody, clone 33D3 (mouse monoclonal antibody) validated in DB, IF, SB, IHC(P), Flow Cytometry, MeDIP to detect 5-methylcytosine also known as methylcytosine, methylcytidine.
      Key Applications
      • Dot Blot
      • Immunofluorescence
      • Southern Blotting
      • Immunohistochemistry (Paraffin)
      • Methylated DNA Immunoprecipitation (MeDIP)
      • Flow Cytometry
      Application NotesIF
      Habib, M., et al. (1999). Exp Cell Res. 249:46-53

      IHC-P
      Hernadez-Blazquez, F.J., et al. (2000). Gut. 47:689-693

      Flow Cytometry
      Reynaud, C.C., et al. (1992). Cancer Lett. 61(3):255-262

      MeDIP
      Keshet, I., et al. (2006). Nat Genet. 38(2):149-153
      Biological Information
      ImmunogenOvalbumin-conjugated 5-methylcytosine
      Clone33D3
      HostMouse
      SpecificityThis antibody detects 5-Methylcytosine, but not unmethylated cytosine.
      IsotypeIgG1λ
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteDemonstrated to react with Human. Predicted to react with multiple species.
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein A
      Molecular WeightDetects methylated cytosine
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Dot Blot in methylated and unmethylated BRCA1.

      Dot Blot Analysis: 1 µg/mL of this antibody detected methylcytosine in methylated and unmethylated BRCA1.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      MSDS

      Título

      Ficha de Segurança de Produtos (MSDS) 

      Certificados de análise

      TítuloNúmero do lote
      Anti-5-methylcytosine, clone 33D3 2475946
      Anti-5-methylcytosine, clone 33D3 - 2105266 2105266
      Anti-5-methylcytosine, clone 33D3 - 2398952 2398952
      Anti-5-methylcytosine, clone 33D3 - 2461586 2461586
      Anti-5-methylcytosine, clone 33D3 - 1982501 1982501
      Anti-5-methylcytosine, clone 33D3 - 2041598 2041598
      Anti-5-methylcytosine, clone 33D3 - 2187393 2187393
      Anti-5-methylcytosine, clone 33D3 - 2187393A 2187393A
      Anti-5-methylcytosine, clone 33D3 - 2204981 2204981
      Anti-5-methylcytosine, clone 33D3 - 2297298 2297298
      Anti-5-methylcytosine, clone 33D3 - 2329182 2329182
      Anti-5-methylcytosine, clone 33D3 - NRG1890922 NRG1890922
      Anti-5-methylcytosine, clone 33D3 -2522248 2522248
      Anti-5-methylcytosine, clone 33D3 -2562010 2562010
      Anti-5-methylcytosine, clone 33D3 -2585926 2585926
      Anti-5-methylcytosine, clone 33D3 -2594421 2594421
      Anti-5-methylcytosine, clone 33D3 -2649284 2649284
      Anti-5-methylcytosine, clone 33D3 -2707234 2707234
      Anti-5-methylcytosine, clone 33D3 -2710542 2710542
      Anti-5-methylcytosine, clone 33D3 -2710543 2710543
      Anti-5-methylcytosine, clone 33D3 -2745940 2745940
      Anti-5-methylcytosine, clone 33D3 -2802021 2802021
      Anti-5-methylcytosine, clone 33D3 -2826074 2826074

      Referências

      Visão geral das referênciasPub Med ID
      Canine spontaneous head and neck squamous cell carcinomas represent their human counterparts at the molecular level.
      Liu, D; Xiong, H; Ellis, AE; Northrup, NC; Dobbin, KK; Shin, DM; Zhao, S
      PLoS genetics 11 e1005277 2015

      Mostrar Resumo
      26030765 26030765
      Chilling- and Freezing-Induced Alterations in Cytosine Methylation and Its Association with the Cold Tolerance of an Alpine Subnival Plant, Chorispora bungeana.
      Song, Y; Liu, L; Feng, Y; Wei, Y; Yue, X; He, W; Zhang, H; An, L
      PloS one 10 e0135485 2015

      Mostrar Resumo
      26270551 26270551
      Induction of sarcomas by mutant IDH2.
      Lu, C; Venneti, S; Akalin, A; Fang, F; Ward, PS; Dematteo, RG; Intlekofer, AM; Chen, C; Ye, J; Hameed, M; Nafa, K; Agaram, NP; Cross, JR; Khanin, R; Mason, CE; Healey, JH; Lowe, SW; Schwartz, GK; Melnick, A; Thompson, CB
      Genes & development 27 1986-98 2013

      Mostrar Resumo
      24065766 24065766
      Evidence for an instructive mechanism of de novo methylation in cancer cells.
      Keshet, Ilana, et al.
      Nat. Genet., 38: 149-53 (2006) 2006

      Mostrar Resumo
      16444255 16444255
      Evaluation of global DNA hypomethylation in human colon cancer tissues by immunohistochemistry and image analysis.
      Hernandez-Blazquez, F J, et al.
      Gut, 47: 689-93 (2000) 2000

      Mostrar Resumo
      11034586 11034586
      DNA global hypomethylation in EBV-transformed interphase nuclei.
      Habib, M, et al.
      Exp. Cell Res., 249: 46-53 (1999) 1999

      Mostrar Resumo
      10328952 10328952
      Methylation of genomes and genes at the invertebrate-vertebrate boundary.
      Tweedie, S, et al.
      Mol. Cell. Biol., 17: 1469-75 (1997) 1997

      Mostrar Resumo
      9032274 9032274
      Polymerase chain reaction products containing 5-methyldeoxycytidine: a microplate immunoquantitation method.
      Niveleau, A, et al.
      Nucleic Acids Res., 22: 5508-9 (1994) 1994

      7816646 7816646
      Abnormal methylation pattern in constitutive and facultative (X inactive chromosome) heterochromatin of ICF patients.
      Miniou, P, et al.
      Hum. Mol. Genet., 3: 2093-102 (1994) 1994

      Mostrar Resumo
      7881405 7881405
      Monitoring of urinary excretion of modified nucleosides in cancer patients using a set of six monoclonal antibodies.
      Reynaud, C, et al.
      Cancer Lett., 61: 255-62 (1992) 1992

      Mostrar Resumo
      1739950 1739950

      Brochura

      Título
      New Products: Volume 3, 2012
      Product Selection Guide - Antibodies, small molecule inhibitors, kits, assays and proteins for signaling research.

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