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MAB1501R | Anti-Actin Antibody, near a.a. 50-70, clone C4

MAB1501R
100 µg  
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      Replacement Information

      Ofertas especiais

      Tabela com principais espec.

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      A ELISA, ICC, IHC, IH(P), WB M Protein G Purified Monoclonal Antibody
      Description
      Catalogue NumberMAB1501R
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Actin Antibody, near a.a. 50-70, clone C4
      Alternate Names
      • actin, alpha 1, skeletal muscle
      • alpha skeletal muscle actin
      • MAB1501
      • MAB1501X
      • smooth muscle actin
      • SMA
      Background InformationActin is an abundant cytoskeletal protein found in all cells (True, 1990). The protein's 42 kD peptide chain assumes two physical forms: globular actin, which may serve as a cytoplasmic storage pool, and fibrous actin, which, in conjunction with myosin, generates muscle contraction (True, 1990). In non-muscle cells, actin appears to be involved in a variety of functions, such as cell motility, exocytosis, and phagocytosis (True, 1990). Distribution of the six known isoforms of actin - four muscle actins (alpha-skeletal, alpha-vascular smooth, alpha-cardiac, and gamma-enteric smooth) and two cytoplasmic actins (alpha and gamma) is tissue specific (Otey, 1986; Lessard, 1988).
      References
      Product Information
      FormatProtein G Purified
      Control
      • HeLa whole cell lysate.
      PresentationProtein G Purified in 0.1M Tris-Glycine (pH 7.4) 150mM NaCl with 0.05% NaN3.
      Applications
      ApplicationThis Anti-Actin Antibody, near a.a. 50-70, clone C4 is validated for use in ELISA, IC, IH, IH(P), WB for the detection of Actin and has published in over 70 citations.
      Key Applications
      • ELISA
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunocytochemistry:
      10 μg/mL dilution from a previous lot was used (methanol fixed mouse 3T3 cells).

      Immunohistochemistry:
      10μg/mL dilution from a previous lot was used for paraffin embedded, 4% formaldehyde, 3% glutaraldehyde, sodium cacodylate treated sections {see Luciano, L et al. 2003}.

      ELISA:
      A previous lot was shown to be strongly reactive with the cytoplasmic actin and shows a significant binding to gizzard, skeletal, arterial and cardiac actins. Also shows a significant binding to both Dictyostelium discoidum and Physarum polycephalum.

      Western blot:
      1-20 µg/ml. On muscle homogenates subject to SDS-PAGE, reacts relatively uniformly with a 43 kD protein present in skeletal, cardiac, gizzard and aorta tissues. Appears to react with all isoforms of actin found in these preparations and shows a strong reaction with the alpha-actin found in skeletal, cardiac, and arterial muscle (Otey, 1987).

      Immunohistochemistry:
      10µg/mL for paraffin embedded, 4% formaldehyde, 3% glutaraldehyde, sodium cacodylate treated sections {see Luciano, L et al. 2003}.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenPurified chicken gizzard actin (Lessard, 1988).
      Epitopea.a. 50-70
      CloneC4
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityMAB1501R is a pan-actin antibody that binds to an epitope in a highly conserved region of actin; therefore, this antibody reacts with all six isoforms of vertabrate actin (Lessard, 1988). The epitope recognized by the antibody appears to be located in the N-terminal two thirds of the actin molecule, possibly near amino acids 50-70. Reacts with both globular (G) and fillimentous (F) forms of actin and does not interfere with actin polymerization to form filaments, at a ratio as high as one antibody per two actin monomers. However, this antibody does increase the extent of polymerization when used at a lower ratio of antibody to actin. In addition to labeling myotubes, anti-actin stains myoblasts and fibroblasts (Lessard, 1983). Although clone C4 is prepared as an antibody to chicken gizzard muscles actin, it reacts with actins from all vertebrates, as well as with Dictyostelium discoideum and Physarum polycephalum actins (Lessard, 1988).
      IsotypeIgG2bκ
      Species Reactivity
      • All
      Species Reactivity NoteTo date, all animal species and cell types with an actin form react by indirect immunofluorescence or immunoblot, including plant actin.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.
      Gene Symbol
      • ACTA1
      • MPFD
      • ASMA
      • NEM1
      • CFTDM
      • ACTA
      • CFTD
      • NEM2
      • Alpha-actin-1
      • CFTD1
      • NEM3
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P68133 # Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
      SIZE: 377 amino acids; 42051 Da
      SUBUNIT: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Interacts with TTID.
      SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton.
      DISEASE: SwissProt: P68133 # Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. Nemaline myopathy (NEM) is a form of congenital myopathy characterized by abnormal thread- or rod-like structures in muscle fibers on histologic examination. The clinical phenotype is highly variable, with differing age at onset and severity. & Defects in ACTA1 are a cause of congenital myopathy with excess of thin myofilaments (CM) [MIM:102610]. & Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
      SIMILARITY: SwissProt: P68133 ## Belongs to the actin family.
      MISCELLANEOUS: In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
      Molecular Weight43 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on HeLa lysate.

      Western Blot Analysis:
      1:1000 dilution of this lot detected actin on 10 μg of HeLa lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 6 months at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      MSDS

      Título

      Ficha de Segurança de Produtos (MSDS) 

      Certificados de análise

      TítuloNúmero do lote
      Anti-Actin, Clone C4 - 2142171 2142171
      Anti-Actin, Clone C4 - 2387816 2387816
      Anti-Actin, Clone C4 - 2391073 2391073
      Anti-Actin, Clone C4 - 2459631 2459631
      Anti-Actin, Clone C4 - 1991258 1991258
      Anti-Actin, Clone C4 - 2020280 2020280
      Anti-Actin, Clone C4 - 2066001 2066001
      Anti-Actin, Clone C4 - 2188493 2188493
      Anti-Actin, Clone C4 - 2279536 2279536
      Anti-Actin, Clone C4 - 2489021 2489021
      Anti-Actin, Clone C4 - LV1611401 LV1611401
      Anti-Actin, Clone C4 - LV1688376 LV1688376
      Anti-Actin, Clone C4 - LV1728681 LV1728681
      Anti-Actin, Clone C4 - LV1746178 LV1746178
      Anti-Actin, Clone C4 - LV1746179 LV1746179
      Anti-Actin, Clone C4 - NG1853933 NG1853933
      Anti-Actin, Clone C4 - NG1867683 NG1867683
      Anti-Actin, Clone C4 -2521458 2521458
      Anti-Actin, Clone C4 -2585812 2585812
      Anti-Actin, Clone C4 -2594388 2594388
      Anti-Actin, Clone C4 -2626041 2626041
      Anti-Actin, Clone C4 -2653087 2653087
      Anti-Actin, Clone C4 -2697553 2697553
      Anti-Actin, Clone C4 -2721700 2721700
      Anti-Actin, Clone C4 -2746338 2746338
      Anti-Actin, Clone C4 -2819194 2819194
      Anti-Actin, near a.a. 50-70, clone C4 - LV1530193 LV1530193

      Referências | 221 Disponível | Ver todas as referências

      Visão geral das referênciasAplicaçãoEspéciesPub Med ID
      Roles of PCNA ubiquitination and TLS polymerases κ and η in the bypass of methyl methanesulfonate-induced DNA damage.
      Wit, N; Buoninfante, OA; van den Berk, PC; Jansen, JG; Hogenbirk, MA; de Wind, N; Jacobs, H
      Nucleic acids research 43 282-94 2015

      Mostrar Resumo
      25505145 25505145
      Sqstm1-GFP knock-in mice reveal dynamic actions of Sqstm1 during autophagy and under stress conditions in living cells.
      Eino, A; Kageyama, S; Uemura, T; Annoh, H; Saito, T; Narita, I; Waguri, S; Komatsu, M
      Journal of cell science 128 4453-61 2015

      Mostrar Resumo
      26483381 26483381
      Hypoxia-induced gene expression results from selective mRNA partitioning to the endoplasmic reticulum.
      Staudacher, JJ; Naarmann-de Vries, IS; Ujvari, SJ; Klinger, B; Kasim, M; Benko, E; Ostareck-Lederer, A; Ostareck, DH; Bondke Persson, A; Lorenzen, S; Meier, JC; Blüthgen, N; Persson, PB; Henrion-Caude, A; Mrowka, R; Fähling, M
      Nucleic acids research 43 3219-36 2015

      Mostrar Resumo
      Western Blotting25753659 25753659
      Wilms' tumor gene 1 regulates p63 and promotes cell proliferation in squamous cell carcinoma of the head and neck.
      Li, X; Ottosson, S; Wang, S; Jernberg, E; Boldrup, L; Gu, X; Nylander, K; Li, A
      BMC cancer 15 342 2015

      Mostrar Resumo
      25929687 25929687
      Human Cytomegalovirus miR-UL112-3p Targets TLR2 and Modulates the TLR2/IRAK1/NFκB Signaling Pathway.
      Landais, I; Pelton, C; Streblow, D; DeFilippis, V; McWeeney, S; Nelson, JA
      PLoS pathogens 11 e1004881 2015

      Mostrar Resumo
      25955717 25955717
      DOT1L cooperates with the c-Myc-p300 complex to epigenetically derepress CDH1 transcription factors in breast cancer progression.
      Cho, MH; Park, JH; Choi, HJ; Park, MK; Won, HY; Park, YJ; Lee, CH; Oh, SH; Song, YS; Kim, HS; Oh, YH; Lee, JY; Kong, G
      Nature communications 6 7821 2015

      Mostrar Resumo
      26199140 26199140
      Sirt1-deficiency causes defective protein quality control.
      Tomita, T; Hamazaki, J; Hirayama, S; McBurney, MW; Yashiroda, H; Murata, S
      Scientific reports 5 12613 2015

      Mostrar Resumo
      26219988 26219988
      Redundant Roles of Rpn10 and Rpn13 in Recognition of Ubiquitinated Proteins and Cellular Homeostasis.
      Hamazaki, J; Hirayama, S; Murata, S
      PLoS genetics 11 e1005401 2015

      Mostrar Resumo
      26222436 26222436
      The unexpected role of polyubiquitin chains in the formation of fibrillar aggregates.
      Morimoto, D; Walinda, E; Fukada, H; Sou, YS; Kageyama, S; Hoshino, M; Fujii, T; Tsuchiya, H; Saeki, Y; Arita, K; Ariyoshi, M; Tochio, H; Iwai, K; Namba, K; Komatsu, M; Tanaka, K; Shirakawa, M
      Nature communications 6 6116 2015

      Mostrar Resumo
      25600778 25600778
      Gq signaling causes glomerular injury by activating TRPC6.
      Wang, L; Jirka, G; Rosenberg, PB; Buckley, AF; Gomez, JA; Fields, TA; Winn, MP; Spurney, RF
      The Journal of clinical investigation 125 1913-26 2015

      Mostrar Resumo
      25844902 25844902

      Ficha de dados

      Título
      Anti-Actin, near a.a. 50-70, clone C4 - Data Sheet

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      MAB1501 Anti-Actin Antibody, clone C4 Preço e disponibilidade

      Categorias

      Life Science Research > Antibodies and Assays > Primary Antibodies