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MABT199 | Anti-Integrin Beta1 Antibody, clone 8E3

MABT199
100 µL  
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      Replacement Information

      Ofertas especiais

      Tabela com principais espec.

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, ACT, IP M Purified Monoclonal Antibody
      Description
      Catalogue NumberMABT199
      DescriptionAnti-Integrin Beta1 Antibody, clone 8E3
      Alternate Names
      • Integrin beta-1
      • Fibronectin receptor subunit beta
      • VLA-4 subunit beta
      • CD29
      Background InformationIntegrin beta1 is a transmembrane glycoprotein that forms noncovalent complexes with various alpha integrin subunits to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metatastatic diffusion of tumor cells. Interactions between integrins and extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression. Phosphorylation of threonines 788 and 789 on integrin beta1 receptor may play a key role in cell-cycle dependent regulation.
      References
      Product Information
      FormatPurified
      Control
      • HT-1080 cell lysate
      PresentationPurified mouse monoclonal IgG1κ in buffer containing PBS without preservatives.
      Applications
      ApplicationThis Anti-Integrin beta1 Antibody is validated for use in WB, IP & activity assays for the detection of Integrin beta1.
      Key Applications
      • Western Blotting
      • Activity Assay
      • Immunoprecipitation
      Application NotesWestern Blot Analysis: A 1:100,000 dilution from a representative lot detected Integrin protein in WB under non-reducing conditions.

      Activity Assay Analysis: A representative lot from an independent laboratory stimulated cellular adhesion and increased ligand binding activity of Integrin Beta1 (Mould, A. P., et al. (2004). J Biol Chem. 280(56):4238-4226.).

      Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Integrin Beta1 in IP (Tiwari, S., et al. (2011). J Cell Sci. 124(10):1672-1680.).
      Biological Information
      ImmunogenPurified Integrin Beta1 from HT-1080 cells.
      EpitopeN-terminus of the PSI domain
      Clone8E3
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes the N-terminus of the PSI domain of Integrin Beta1. Under native conditions, clone 8E3 is shown to recognize the unbent, monomeric ER form of Integrin Beta1 at the PSI domain; however, the epitope only becomes exposed in the Golgi form after denaturation. This observation suggests that the epitopes are present in monomeric beta 1-integrins, but become lost following assembly with alpha-integrin subunits (Tiwari, S., et al. (2011). J Cell Sci. 124(10):1672-1680.; Mould, A. P., et al. (2004). J Biol Chem. 280(56):4238-4226.)
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ITGB
      • FNRB
      • MDF2
      • MSK12
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~100-150 kDa observed. The calculated molecular weight is 86 kDa; however, it is known to have multiple isoforms that range between ~87-91 kDa. This protein is highly glycosolated and depending on the cell line or tissue, it has been observed at ~100-150 kDa (Bridger, P. S., et al. (2008). Biol Reprod. 79(2):274-282.).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HT-1080 cell lysate.

      Western Blot Analysis: A 1:2,000 dilution of this antibody detected Integrin Beta1 in 10 µg of HT-1080 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      MSDS

      Título

      Ficha de Segurança de Produtos (MSDS) 

      Certificados de análise

      TítuloNúmero do lote
      Anti-Integrin Beta1, clone 8E3 - 2463681 2463681
      Anti-Integrin Beta1, clone 8E3 - 2492164 2492164
      Anti-Integrin Beta1, clone 8E3 - Q2054372 Q2054372
      Anti-Integrin Beta1, clone 8E3 -2535301 2535301
      Anti-Integrin Beta1, clone 8E3 -2556309 2556309
      Anti-Integrin Beta1, clone 8E3 -2621112 2621112
      Anti-Integrin Beta1, clone 8E3 -2710208 2710208
      Anti-Integrin Beta1, clone 8E3 -2765745 2765745

      Referências

      Visão geral das referênciasAplicaçãoPub Med ID
      Divalent cations regulate the folding and activation status of integrins during their intracellular trafficking.
      Tiwari, Shweta, et al.
      J. Cell. Sci., 124: 1672-80 (2011) 2011

      Mostrar Resumo
      21511727 21511727
      Integrin activation in bovine placentomes and in caruncular epithelial cells isolated from pregnant cows.
      Bridger, PS; Haupt, S; Leiser, R; Johnson, GA; Burghardt, RC; Tinneberg, HR; Pfarrer, C
      Biology of reproduction 79 274-82 2008

      Mostrar Resumo
      Immunofluorescence18417711 18417711
      Evidence that monoclonal antibodies directed against the integrin beta subunit plexin/semaphorin/integrin domain stimulate function by inducing receptor extension.
      Mould, A Paul, et al.
      J. Biol. Chem., 280: 4238-46 (2005) 2005

      Mostrar Resumo
      15557320 15557320

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      Categorias

      Life Science Research > Antibodies and Assays > Primary Antibodies