A part of Merck

AB1991 | Anti-Neurofilament H (200 kDa) Antibody, lysine-serine-proline repeat

AB1991
50 µL  
Recuperando preço...
O preço não pôde ser recuperado
Quantidade mínima necessária para ser múltipla de
Após finalização do pedido Mais informações
Você salvou ()
 
Solicitar preço
Disponibilidade limitadaDisponibilidade limitada
Em estoque 
Descontinuado
Quantidades limitadas disponíveis
Disponibilidade a ser confirmada
    Restante: será informado
      Restante: será informado
      Vai informar
      Contatar o serviço de atendimento ao cliente

      Ofertas especiais

       

      Contatar o serviço de atendimento ao cliente

      Click To Print This Page

      Panorama geral

      Replacement Information

      Ofertas especiais

      Tabela com principais espec.

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Av, Ma, Rp IH(P), WB, IHC Rb Serum Polyclonal Antibody
      Description
      Catalogue NumberAB1991
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Neurofilament H (200 kDa) Antibody, lysine-serine-proline repeat
      Background InformationNeurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexs of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments. For more neurofilament information see Nervous System Cell Type Specific Marker chart online under the CHEMICON Technical Support section.
      References
      Product Information
      FormatSerum
      PresentationNeat rabbit antisera.
      Applications
      ApplicationDetect Neurofilament H (200 kDa) using this Anti-Neurofilament H (200 kDa) Antibody, lysine-serine-proline repeat validated for use in IH(P), WB, IH.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunohistochemistry: 1:250-1:1,000 AB1991 is not affected by the level of neurofilament phosphorylation and is particularly good for revealing dendritic and perikaryal neurofilaments.

      Immunoblotting

      Electron microscopy

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenE. Coli recombinant fusion protein containing 37 lysine-serine-proline repeats of rat NF-H.
      Epitopelysine-serine-proline repeat
      HostRabbit
      SpecificityStrong reactivity to the major neurofilament subunit HF-H. Since a second neurofilament subunit, NF-M, also contains a few lysine-serine-proline (KSP) sequences, there is generally some reactivity with this protein also. AB1991 stains both phosphorylated and dephosphorylated neurofilaments.
      Species Reactivity
      • Avian
      • Mammals
      • Reptile
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NEFH
      • NFH
      • NF-H
      • KIAA0845
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P12036 # Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
      SIZE: 1026 amino acids; 112480 Da
      PTM: There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. & Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
      DISEASE: SwissProt: P12036 # Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.
      SIMILARITY: SwissProt: P12036 ## Belongs to the intermediate filament family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size50 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      MSDS

      Título

      Ficha de Segurança de Produtos (MSDS) 

      Certificados de análise

      TítuloNúmero do lote
      RABBIT ANTI-NEUROFILAMENT H (LYSINE-SERINE-PROLINE REPEAT) POLYCLONAL ANTIBODY - 2428519 2428519
      RABBIT ANTI-NEUROFILAMENT H (LYSINE-SERINE-PROLINE REPEAT) POLYCLONAL ANTIBODY - 2462740 2462740
      RABBIT ANTI-NEUROFILAMENT H (LYSINE-SERINE-PROLINE REPEAT) POLYCLONAL ANTIBODY - 2070399 2070399
      RABBIT ANTI-NEUROFILAMENT H (LYSINE-SERINE-PROLINE REPEAT) POLYCLONAL ANTIBODY - 2234135 2234135
      RABBIT ANTI-NEUROFILAMENT H -2572992 2572992
      RABBIT ANTI-NEUROFILAMENT H -2610967 2610967
      RABBIT ANTI-NEUROFILAMENT H -2728423 2728423
      RABBIT ANTI-NEUROFILAMENT H -2776886 2776886

      Referências | 28 Disponível | Ver todas as referências

      Visão geral das referênciasAplicaçãoEspéciesPub Med ID
      An NT4/TrkB-dependent increase in innervation links early-life allergen exposure to persistent airway hyperreactivity.
      Aven, L; Paez-Cortez, J; Achey, R; Krishnan, R; Ram-Mohan, S; Cruikshank, WW; Fine, A; Ai, X
      FASEB journal : official publication of the Federation of American Societies for Experimental Biology 28 897-907 2014

      Mostrar Resumo
      Western Blotting24221086 24221086
      Antibodies against low-density lipoprotein receptor-related protein 4 induce myasthenia gravis.
      Shen, C; Lu, Y; Zhang, B; Figueiredo, D; Bean, J; Jung, J; Wu, H; Barik, A; Yin, DM; Xiong, WC; Mei, L
      The Journal of clinical investigation 123 5190-202 2013

      Mostrar Resumo
      24200689 24200689
      Rescue of peripheral and CNS axon defects in mice lacking NMNAT2.
      Gilley, J; Adalbert, R; Yu, G; Coleman, MP
      The Journal of neuroscience : the official journal of the Society for Neuroscience 33 13410-24 2013

      Mostrar Resumo
      23946398 23946398
      β-Catenin gain of function in muscles impairs neuromuscular junction formation.
      Wu, H; Lu, Y; Barik, A; Joseph, A; Taketo, MM; Xiong, WC; Mei, L
      Development (Cambridge, England) 139 2392-404 2012

      Mostrar Resumo
      22627288 22627288
      Distinct roles of muscle and motoneuron LRP4 in neuromuscular junction formation.
      Wu, H; Lu, Y; Shen, C; Patel, N; Gan, L; Xiong, WC; Mei, L
      Neuron 75 94-107 2012

      Mostrar Resumo
      Western Blotting22794264 22794264
      VEGF-Induced Growth Cone Enhancement Is Diminished by Inhibiting Tyrosine-Residue 1214 of VEGFR-2.
      Daniel Foehring,Beate Brand-Saberi,Carsten Theiss
      Cells, tissues, organs 196 2012

      Mostrar Resumo
      22433970 22433970
      Astrocyte-secreted factors modulate the developmental distribution of inhibitory synapses in nucleus laminaris of the avian auditory brainstem.
      Matthew J Korn,Scott J Koppel,Lan H Li,Divya Mehta,Sonia B Mehta,Armin H Seidl,Karina S Cramer
      The Journal of comparative neurology 520 2012

      Mostrar Resumo
      22020566 22020566
      Olig1 function is required for remyelination potential of transplanted neural progenitor cells in a model of viral-induced demyelination.
      Whitman, LM; Blanc, CA; Schaumburg, CS; Rowitch, DH; Lane, TE
      Experimental neurology 235 380-7 2012

      Mostrar Resumo
      Immunohistochemistry22449475 22449475
      A method to isolate and culture expand human dental pulp stem cells.
      Stan Gronthos,Agnieszka Arthur,P Mark Bartold,Songtao Shi
      Methods in molecular biology (Clifton, N.J.) 698 2011

      Mostrar Resumo
      21431514 21431514
      Spreading of prions from the immune to the peripheral nervous system: a potential implication of dendritic cells.
      Dorban, Gauthier, et al.
      Histochem. Cell Biol., 133: 493-504 (2010) 2010

      Mostrar Resumo
      20238136 20238136

      Ficha de dados

      Título
      RABBIT ANTI-NEUROFILAMENT H

      Produtos e aplicações relacionadas

      Related Products

      Número de catálogo Descrição  
      MAB5266 Anti-Neurofilament 200 kDa Antibody, clone N52 Preço e disponibilidade
      AB1989 Anti-Neurofilament H (200 kDa) Antibody, CT Preço e disponibilidade

      Famílias de produtos

      Categorias

      Life Science Research > Antibodies and Assays > Primary Antibodies