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MAB3408 | Anti-Tubulin Antibody, beta, clone KMX-1

MAB3408
50 µg  
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      Replacement Information

      Ofertas especiais

      Tabela com principais espec.

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      A, H, M, R ICC, WB M Purified Monoclonal Antibody
      Description
      Catalogue NumberMAB3408
      Replaces04-1049
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Tubulin Antibody, beta, clone KMX-1
      Alternate Names
      • Tubulin beta-III
      • tubulin, beta 3
      • tubulin, beta 4
      Background InformationMicrotubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 kDa. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta Tubulin may not be stable in certain cells. For example, expression of tubulin in adipose tissue is very low and therefore beta Tubulin should not be used as loading control for these tissues.
      References
      Product Information
      FormatPurified
      Control
      • HeLa Cell lysate
      • A431 Cell lysate
      • MCF7 Cell lysate
      PresentationPurified mouse monoclonal IgG2b liquid in buffer containing 0.02M phosphate buffer, 0.25M NaCl with 0.1% sodium azide.
      Applications
      ApplicationThis Anti-Tubulin Antibody, beta, clone KMX-1 is validated for use in IC, WB for the detection of Tubulin.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      Application NotesImmunocytochemistry:
      A 1-2 μg/mL concentration of a previous lot was used in immunocytochemistry.

      Western blot:
      0.05-0.5 μg/mL

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenTubulin purified from polycephalum myxamoebae
      CloneKMX-1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody reacts specifically with β-tubulin and shows a strong cross-reactivity to different species. This antibody allows staining of microtubuli using indirect immunofluorescence showing a low background only.
      IsotypeIgG2b
      Species Reactivity
      • All
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TUBB3
      • TUBB4
      • MC1R
      • beta-4
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q13509 # Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
      SIZE: 450 amino acids; 50433 Da
      SUBUNIT: Dimer of alpha and beta chains.
      DOMAIN: SwissProt: Q13509 The highly acidic C-terminal region may bind cations such as calcium.
      SIMILARITY: Belongs to the tubulin family.
      Molecular Weight50 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on A431 lysates.

      Western blot:
      1:500 dilution of this lot detected TUBULIN (BETA) on 10 μg of A431 lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 6 months at 2-8ºC in undiluted aliquots from date of receipt.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      MSDS

      Título

      Ficha de Segurança de Produtos (MSDS) 

      Certificados de análise

      TítuloNúmero do lote
      Anti-Tubulin, beta, clone KMX-1 - 2452493 2452493
      Anti-Tubulin, beta, clone KMX-1 - 2462845 2462845
      Anti-Tubulin, beta, clone KMX-1 - 2022031 2022031
      Anti-Tubulin, beta, clone KMX-1 - 2049797 2049797
      Anti-Tubulin, beta, clone KMX-1 - 2090933 2090933
      Anti-Tubulin, beta, clone KMX-1 - 2191969 2191969
      Anti-Tubulin, beta, clone KMX-1 - 2298363 2298363
      Anti-Tubulin, beta, clone KMX-1 - LV1475931 LV1475931
      Anti-Tubulin, beta, clone KMX-1 - LV1584912 LV1584912
      Anti-Tubulin, beta, clone KMX-1 - LV1649527 LV1649527
      Anti-Tubulin, beta, clone KMX-1 - LV1706242 LV1706242
      Anti-Tubulin, beta, clone KMX-1 - LV1770313 LV1770313
      Anti-Tubulin, beta, clone KMX-1 - NG1879015 NG1879015
      Anti-Tubulin, beta, clone KMX-1 -2489027 2489027
      Anti-Tubulin, beta, clone KMX-1 -2549089 2549089
      Anti-Tubulin, beta, clone KMX-1 -2576301 2576301
      Anti-Tubulin, beta, clone KMX-1 -2616155 2616155
      Anti-Tubulin, beta, clone KMX-1 -2646950 2646950
      Anti-Tubulin, beta, clone KMX-1 -2669951 2669951
      Anti-Tubulin, beta, clone KMX-1 -2727234 2727234
      Anti-Tubulin, beta, clone KMX-1 -2780473 2780473

      Referências | 62 Disponível | Ver todas as referências

      Visão geral das referênciasAplicaçãoEspéciesPub Med ID
      Zinc finger independent genome-wide binding of Sp2 potentiates recruitment of histone-fold protein Nf-y distinguishing it from Sp1 and Sp3.
      Völkel, S; Stielow, B; Finkernagel, F; Stiewe, T; Nist, A; Suske, G
      PLoS genetics 11 e1005102 2015

      Mostrar Resumo
      25793500 25793500
      Shifts in temperature within the physiologic range modify strand-specific expression of select human microRNAs.
      Potla, R; Singh, IS; Atamas, SP; Hasday, JD
      RNA (New York, N.Y.) 21 1261-73 2015

      Mostrar Resumo
      26018549 26018549
      Type-1 interferons contribute to oxygen glucose deprivation induced neuro-inflammation in BE(2)M17 human neuroblastoma cells.
      Minter, MR; Zhang, M; Ates, RC; Taylor, JM; Crack, PJ
      Journal of neuroinflammation 11 43 2014

      Mostrar Resumo
      24602263 24602263
      The sphingolipid receptor S1PR2 is a receptor for Nogo-a repressing synaptic plasticity.
      Kempf, A; Tews, B; Arzt, ME; Weinmann, O; Obermair, FJ; Pernet, V; Zagrebelsky, M; Delekate, A; Iobbi, C; Zemmar, A; Ristic, Z; Gullo, M; Spies, P; Dodd, D; Gygax, D; Korte, M; Schwab, ME
      PLoS biology 12 e1001763 2014

      Mostrar Resumo
      24453941 24453941
      Comparative analysis of single and combined APP/APLP knockouts reveals reduced spine density in APP-KO mice that is prevented by APPsα expression.
      Weyer, SW; Zagrebelsky, M; Herrmann, U; Hick, M; Ganss, L; Gobbert, J; Gruber, M; Altmann, C; Korte, M; Deller, T; Müller, UC
      Acta neuropathologica communications 2 36 2014

      Mostrar Resumo
      24684730 24684730
      Localization of heat shock proteins in cerebral cortical cultures following induction by celastrol.
      Chow, AM; Tang, DW; Hanif, A; Brown, IR
      Cell stress & chaperones 19 845-51 2014

      Mostrar Resumo
      24700193 24700193
      Nucleosome positioning and histone modifications define relationships between regulatory elements and nearby gene expression in breast epithelial cells.
      Rhie, SK; Hazelett, DJ; Coetzee, SG; Yan, C; Noushmehr, H; Coetzee, GA
      BMC genomics 15 331 2014

      Mostrar Resumo
      Western Blotting24885402 24885402
      Contactin-1 regulates myelination and nodal/paranodal domain organization in the central nervous system.
      Çolakoğlu, G; Bergstrom-Tyrberg, U; Berglund, EO; Ranscht, B
      Proceedings of the National Academy of Sciences of the United States of America 111 E394-403 2014

      Mostrar Resumo
      24385581 24385581
      Stress-induced localization of HSPA6 (HSP70B') and HSPA1A (HSP70-1) proteins to centrioles in human neuronal cells.
      Khalouei, S; Chow, AM; Brown, IR
      Cell stress & chaperones 19 321-7 2014

      Mostrar Resumo
      Western Blotting24061851 24061851
      Induction of heat shock proteins in cerebral cortical cultures by celastrol.
      Chow, AM; Tang, DW; Hanif, A; Brown, IR
      Cell stress & chaperones 18 155-60 2013

      Mostrar Resumo
      22865541 22865541

      Ficha de dados

      Título
      Anti-Tubulin, beta, clone KMX-1 - Data Sheet

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      Categorias

      Life Science Research > Antibodies and Assays > Primary Antibodies