A part of Merck

07-540 | Anti-acetyl-Histone H3 (Lys36) Antibody

07-540
100 µL  
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      Replacement Information

      Ofertas especiais

      Tabela com principais espec.

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, Mplex Rb Serum Polyclonal Antibody
      Description
      Catalogue Number07-540
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-acetyl-Histone H3 (Lys36) Antibody
      Alternate Names
      • H3K36Ac
      • Histone H3 (acetyl K36)
      References
      Product Information
      FormatSerum
      Presentation100μl of rabbit antiserum containing 0.05% sodium azide and 30% glycerol.
      Applications
      ApplicationUse Anti-acetyl-Histone H3 (Lys36) Antibody (Rabbit Polyclonal Antibody) validated in WB, Mplex to detect acetyl-Histone H3 (Lys36) also known as H3K36Ac, Histone H3 (acetyl K36).
      Key Applications
      • Western Blotting
      • Multiplexing
      Application NotesSuggested dilutions:
      Western Blot: 1:5000 - 1:20,000

      ChIP Analysis: A representative lot was used by an independent laboratory for ChIP (Gatta, R., et al. (2011). Epigenetics. 6(4):526-534.)
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide containing the sequence ...GV[AcK]KP... in which [AcK] corresponds to acetyl-lysine 36 of human histone H3.
      HostRabbit
      SpecificityRecognizes Histone H3 when acetylated on Lys36
      Species Reactivity
      • Human
      Species Reactivity NoteBroad species cross reactivity is expected
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family.
      Gene Symbol
      • H3F3A
      • MGC87783
      • H3.3A
      • MGC87782
      • H3F3
      • H3.3B
      • H3F3B
      Modifications
      • Acetylation
      Purification MethodAntiserum
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 136 amino acids; 15508 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      SUBCELLULAR LOCATION: Nucleus.
      PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity).
      SIMILARITY: SwissProt: Q16695 ## Belongs to the histone H3 family.
      Molecular Weight~17kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C from date of shipment
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      MSDS

      Título

      Ficha de Segurança de Produtos (MSDS) 

      Certificados de análise

      TítuloNúmero do lote
      Anti-acetyl-Histone H3 (Lys36) 2465199
      Anti-acetyl-Histone H3 (Lys36) - 26750 26750

      Referências

      Visão geral das referênciasAplicaçãoEspéciesPub Med ID
      The histone acetyltransferase MOF activates hypothalamic polysialylation to prevent diet-induced obesity in mice.
      Brenachot, X; Rigault, C; Nédélec, E; Laderrière, A; Khanam, T; Gouazé, A; Chaudy, S; Lemoine, A; Datiche, F; Gascuel, J; Pénicaud, L; Benani, A
      Molecular metabolism 3 619-29 2014

      Mostrar Resumo
      25161885 25161885
      Histone acetyltransferase Enok regulates oocyte polarization by promoting expression of the actin nucleation factor spire.
      Huang, F; Paulson, A; Dutta, A; Venkatesh, S; Smolle, M; Abmayr, SM; Workman, JL
      Genes & development 28 2750-63 2014

      Mostrar Resumo
      25512562 25512562
      Cluster analysis reveals differential transcript profiles associated with resistance training-induced human skeletal muscle hypertrophy.
      Thalacker-Mercer, A; Stec, M; Cui, X; Cross, J; Windham, S; Bamman, M
      Physiological genomics 45 499-507 2013

      Mostrar Resumo
      23632419 23632419
      Endoplasmic reticulum stress-associated cone photoreceptor degeneration in cyclic nucleotide-gated channel deficiency.
      Thapa, A; Morris, L; Xu, J; Ma, H; Michalakis, S; Biel, M; Ding, XQ
      The Journal of biological chemistry 287 18018-29 2012

      Mostrar Resumo
      Western BlottingMouse22493484 22493484
      Lysine-specific demethylase 1 regulates the embryonic transcriptome and CoREST stability.
      Foster, CT; Dovey, OM; Lezina, L; Luo, JL; Gant, TW; Barlev, N; Bradley, A; Cowley, SM
      Molecular and cellular biology 30 4851-63 2010

      Mostrar Resumo Texto completo do artigo
      Western Blotting20713442 20713442
      Histone acetylation: a switch between repressive and permissive chromatin. Second in review series on chromatin dynamics.
      Eberharter, Anton and Becker, Peter B
      EMBO Rep., 3: 224-9 (2002) 2002

      Mostrar Resumo
      11882541 11882541

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      Categorias

      Life Science Research > Antibodies and Assays > Primary Antibodies