A part of Merck

S7701 | TRAPeze® Telomerase Positive Control Cell Pellet

S7701
1 vial  1x10(e6) cells
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      Description
      Catalogue NumberS7701
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionTRAPeze® Telomerase Positive Control Cell Pellet
      OverviewThis product is intended for use with the components of the TRAPEZE® Telomerase Detection Kit (Catalog # S7700), the TRAPEZE® XL Telomerase Detection Kit (Catalog # S7707) and the TRAPEZE® ELISA Telomerase Detection Kit (Catalog # S7750). Recommended Taq polymerases: must be non-proofreading, having no exonuclease activity and capable of "hot-start." Titanium Taq™, Platinum® Taq are suggested.



      Material Provided

      Each vial of S7701 TRAPEZE® Positive Control Cell Pellet contains one million cells, pelletted to the bottom of the microcentrifuge tube.
      References
      Product Information
      Applications
      ApplicationThis product is intended for use with the components of the TRAPEZE Telomerase Detection Kit, the TRAPEZE XL Telomerase Detection Kit & the TRAPEZE ELISA Telomerase Detection Kit.
      Application NotesInstructions for Use

      1. Resuspend the cell pellet in 200 μl of 1X CHAPS Lysis Buffer.

      2. Incubate the suspension on ice for 30 minutes.

      3. Spin the sample in a microcentrifuge at 12,000 X g for 20 minutes at 4°C.

      4. Transfer 160 μl of the supernatant into a fresh tube.

      5. Aliquot and quick-freeze the remaining extract on dry ice, and store at -75°C to -85°C. The extracts for the TRAP assay should be quick-frozen on dry ice after each use. Aliquots should not be freeze-thawed more than 5 times to avoid loss of telomerase activity. Additionally, aliquoting reduces the risk of contamination.

      6. Use 1000 cell equivalents (2 μl of a 1:10 dilution) per TRAP assay, according to the kit manuals.
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMust be stored at -75°C to -85°C. Telomerase in specimens stored as a cell pellet is stable at this temperature for several years. Once the cell extract is made (in 1X CHAPS Lysis Buffer), it is stable for 1 year, if not subjected to more than 5 freeze-thaw cycles
      Packaging Information
      Material Size1 vial
      Material Package1x10(e6) cells
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificados de análise

      TítuloNúmero do lote
      Telomerase Positive Control 2833526
      Telomerase Positive Control - 2392288 2392288
      Telomerase Positive Control - 2064552 2064552
      Telomerase Positive Control - 2200944 2200944
      Telomerase Positive Control - 2206396 2206396
      Telomerase Positive Control - 2283339 2283339
      Telomerase Positive Control - 2533221 2533221
      Telomerase Positive Control -2575144 2575144
      Telomerase Positive Control -2649211 2649211

      Referências

      Visão geral das referênciasPub Med ID
      Inhibition of telomerase by G-quartet DNA structures.
      A M Zahler,J R Williamson,T R Cech,D M Prescott
      Nature 350 1991

      Mostrar Resumo
      2023635 2023635

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