AB3852 Anti-Cortactin Antibody, phospho-specific (Tyr421)

AB3852
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB3852
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Cortactin Antibody, phospho-specific (Tyr421)
      References
      Product Information
      FormatAffinity Purified
      PresentationAffinity purified immunoglobulin. Liquid in 1mg/mL in PBS with 0.05% Bronidox.
      Applications
      ApplicationAnti-Cortactin Antibody, phospho-specific (Tyr421) detects level of Cortactin & has been published & validated for use in WB.
      Key Applications
      • Western Blotting
      Application NotesWestern blot: 1-2 μg/mL

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenRabbit anti-Cortactin (Y421) polyclonal antibody was raised against a peptide corresponding to residues surrounding phosphorylated Y421of human cortactin.
      Epitopephospho-specific (Tyr421)
      HostRabbit
      SpecificityRecognizes Cortactin phosphorylated on tyrosine 421. Cortactin is an 80-85 kDa actin-binding protein, which consists of a variety of protein domains. The nature and function of these domains, coupled with the presence of multiple consensus sites for phosphorylation by tyrosine and serine/threonine kinases, suggests that cortactin integrates activation of specific signaling pathways with cytoskeletal reorganization.
      Species Reactivity
      • Human
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Two splice variants that encode different isoforms have been identified for this gene.
      Gene Symbol
      • CTTN
      • EMS1
      • Amplaxin
      • 1110020L01Rik
      • FLJ34459
      Modifications
      • Phosphorylation
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q14247 # May contribute to the organization of cell structure. The SH3 motif may function as a binding region to cytoskeleton. Tyrosine phosphorylation in transformed cells may contribute to cellular growth regulation and transformation.
      SIZE: 550 amino acids; 61636 Da
      SUBUNIT: Interacts with SHANK2 and SHANK3 via its SH2 domain. Also interacts with FGD1 (By similarity). Interacts with PLXDC2.
      SUBCELLULAR LOCATION: Cytoplasm (By similarity). Cell projection, lamellipodium (By similarity). Cell projection, ruffle (By similarity). Note=Associated with membrane ruffles and lamellipodia (By similarity).
      SIMILARITY: SwissProt: Q14247 ## Contains 7 cortactin repeats. & Contains 1 SH3 domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months after receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Cortactin Antibody, phospho-specific (Tyr421) Certificates of Analysis

      TitleLot Number
      Rabbit Anti Cortactin (Y421) Polyclonal Antibody (100ug) - 32182333218233
      Rabbit Anti Cortactin (Y421) Polyclonal Antibody (100ug) - ME8MN1287ME8MN1287
      Rabbit Anti Cortactin (Y421) Polyclonal Antibody (100ug) - ME9AN8755ME9AN8755

      References

      Reference overviewPub Med ID
      Hippocampal Cortactin Levels are Reduced Following Spatial Working Memory Formation, an Effect Blocked by Chronic Calpain Inhibition.
      Olson, ML; Ingebretson, AE; Harmelink, KM
      Brain sciences  5  241-57  2015

      Show Abstract
      26103422 26103422
      Phosphorylation of N-cadherin-associated cortactin by Fer kinase regulates N-cadherin mobility and intercellular adhesion strength.
      El Sayegh, TY; Arora, PD; Fan, L; Laschinger, CA; Greer, PA; McCulloch, CA; Kapus, A
      Molecular biology of the cell  16  5514-27  2005

      Show Abstract Full Text Article
      16176974 16176974
      Cortactin associates with N-cadherin adhesions and mediates intercellular adhesion strengthening in fibroblasts.
      El Sayegh, TY; Arora, PD; Laschinger, CA; Lee, W; Morrison, C; Overall, CM; Kapus, A; McCulloch, CA
      Journal of cell science  117  5117-31  2004

      Show Abstract
      15383621 15383621

      Data Sheet

      Title
      Anti-Cortactin, phospho-specific (Tyr421) - Data Sheet

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies