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SCT120 BioTracker 488 Green Nuclear Dye

Live cell imaging green nuclear staining dye with greater photostability than traditional blue fluorescent nuclear stains such as DAPI and Hoechst 33342.

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SCT120
1 Kit  
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      Descripción

      Replacement Information
      Description
      Catalogue NumberSCT120
      DescriptionBioTracker 488 Green Nuclear Dye
      OverviewThe nucleus is a membrane-enclosed organelle found in eukaryotic cells. Cell nuclei contain most of the cell's genetic material, organized as multiple long linear DNA molecules in complex with a large variety of proteins, such as histones, to form chromosomes. Traditionally, DNA stains such as DAPI and Hoechst have been used for microscopy but require fixation and cannot be used for live cell imaging.

      The BioTracker 488 Green Nuclear Dye is a cell-membrane permeable green fluorescent DNA dye that specifically stains nuclei in live or fixed cells. It has excellent specificity for DNA without the need for a wash step, and it has low toxicity for live cell imaging. The dye is supplied with a vial of verapamil, an efflux pump inhibitor that may improve probe retention and live cell staining in certain cell types.

      Note: BioTracker™ Nuclear Dyes also shows blue fluorescence in the DAPI channel, and may not be suitable for multicolor imaging with blue probes.

      Spectral Properties
      Absorbance: 500nm
      Emission: 515nm
      Alternate Names
      • Live cell imaging probe
      References
      Product Information
      Components
      • 1) 1 vial of 50µL BioTracker 488 Green Nuclear Dye (1000X in DMSO) (CS224594)
      • 2) 1 vial of 100µL Verapamil HCL (100mM in DMSO) (CS224592)
      Detection methodFluorescence
      PresentationLiquid
      Applications
      ApplicationLive cell imaging green nuclear staining dye with greater photostability than traditional blue fluorescent nuclear stains such as DAPI and Hoechst 33342.
      Key Applications
      • Cell Based Assays
      Application NotesLive cell fluorescent imaging
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceSpectral Properties
      Absorbance: 500nm
      Emission: 515nm
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore BioTracker 488 Green Nuclear Dye at 2-8ºC. Protect From Light.
      Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
      Packaging Information
      Material Size1 Kit
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      SCT120 04054839566943