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HCCB4MAG-58K MILLIPLEX MAP Human Circulating Cancer Biomarker Magnetic Bead Panel 4 - Cancer Multiplex Assay

MILLIPLEX® MAP Human Circulating Cancer Biomarker Panel 4 is a Bead-Based Multiplex Assay using the Luminex xMAP® technology, enabling the simultaneous analysis of multiple cancer biomarkers in human serum, plasma, and cellular tissue culture samples.

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Key Applications: Multiplexing Detection Methods: Luminex xMAP Research Category: Cancer Research Sub-Category: Immune Response, Alzheimer's Disease, Inflammation, Oncology, Tumor Markers
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HCCB4MAG-58K
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Descripción

Replacement Information

Tabla espec. clave

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
ALDH1A1 CD44 Hepsin L1CAM Mesothelin Transglutaminase 2 (TGM2) Carbonic Anhydrase IX (CA9) EpCam Kallikrein-6 Midkine HMplexLuminex xMAP
Description
Catalogue NumberHCCB4MAG-58K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX MAP Human Circulating Cancer Biomarker Magnetic Bead Panel 4 - Cancer Multiplex Assay
OverviewEMD Millipore’s MILLIPLEX® MAPHuman Circulating Cancer Biomarker Magnetic Bead Panel4 to be used for the simultaneous quantification of the following analytes in serum, plasma or culture supernatant samples:ALDH1A1, CD44, hepsin, L1CAM, mesothelin, transglutaminase 2 (TGM2), carbonic anhydrase IX (CA9), epCam, kallikrein-6, and midkine.

MILLIPLEX® MAP offers the broadest selection of analytes across a wide range of disease states and species. Once the analytes of interest have been identified, you can rely on the quality that we build into each kit to produce results you can trust. In addition to the assay characteristics listed in the protocol, other performance criteria evaluated during the validation process include: cross-reactivity, dilution linearity, kit stability, and sample behavior (e.g. detectability and stability). Each panel and kit meets stringent manufacturing criteria to ensure batch-to-batch reproducibility.

Each MILLIPLEX® MAP panel and kit includes:
• Quality controls (QCs) provided to qualify assay performance

• Comparison of standard (calibrator) and QC lots to a reference lot to ensure lot-to-lot consistency

• Optimized serum matrix to mimic native analyte environment

• Detection antibody cocktails designed to yield consistent analyte profiles within panel


MILLIPLEX® MAP offers you:
o The ability to choose any combination of analytes from our panel of seven analytes to design a custom kit that better meets your needs.
o A convenient “all-in-one” box format that gives you the assurance that you will have all the necessary reagents you need to run your assay.

EMD Millipore’s MILLIPLEX® MAP Human Diabetes Magnetic Bead Panel is part of the most versatile system available for complement factor research. From our single to multiplex biomarker solutions, we partner with you to design, develop, analytically validate and build the most comprehensive library available for protein detection and quantitation.
Background InformationCancer research has always focused on intracellular biomarkers, such as HER2 and B-Raf, and in recent years, more and more attention has shifted to include extracellular cancer biomarkers. While the use of intracellular markers in research helps clarify the process of oncogenesis, circulating cancer biomarkers give insight into how the body responds to the presence of a tumor, the dysregulation of homeostasis, and the relationship between a tumor and its environment. Study of isolated biomarkers, whether intracellular or circulating, is often inadequate to fully characterize the complex relationship between tumor and non-tumor. Consequently, multianalyte panels have become essential tools that enable researchers to measure cancer biomarkers simultaneously, teasing out these relationships and applying them to understand tumor biology.
References
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Intra-assay: < 20%
  • Inter-assay: < 10
Panel TypeMAGNETIC Circulating Cancer
Quality LevelMQ200
Applications
ApplicationMILLIPLEX® MAP Human Circulating Cancer Biomarker Panel 4 is a Bead-Based Multiplex Assay using the Luminex xMAP® technology, enabling the simultaneous analysis of multiple cancer biomarkers in human serum, plasma, and cellular tissue culture samples.
Key Applications
  • Multiplexing
Application NotesThis is an overnight incubation assay.

A maximum of 25 μL per well of 1:5 diluted serum or plasma can be used. Tissue culture supernatant may require a dilution with an appropriate control medium prior to assay.

This kit may be used for the analysis of all or any combination of the Human Circulating Cancer Biomarker panel 4 analytes in serum, plasma or tissue/cell/tumor culture supernatant.

Please note that min DC for CA9, Midkine, Kallikrein 6 and EpCAM are in pg/mL while the rest of the analyte min DC are in ng/mL.
Biological Information
SpecificityThere was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.
Species Reactivity
  • Human
Analytes Available
  • ALDH1A1
  • CD44
  • Hepsin
  • L1CAM
  • Mesothelin
  • Transglutaminase 2 (TGM2)
  • Carbonic Anhydrase IX (CA9)
  • EpCam
  • Kallikrein-6
  • Midkine
Assay PanelEverything you need in a single kit.
Cross ReactivityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Accuracy
  • 80%-110%
Physicochemical Information
Sensitivity
  • Refer to kit protocol for sensitivities of individual biomarkers.
Linearity of DilutionContact technical support for this information
Standard Curve Range
  • ALDH1A1: 2.7 - 2,000 ng/mL
  • CD44: 1.4 - 1,000 ng/mL
  • Hepsin: 0.3 - 250 ng/mL
  • L1CAM: 0.1 - 100 ng/mL
  • Mesothelin: 0.1 - 100 ng/mL
  • Transglutaminase 2 (TGM2): 1.4 - 1,000 ng/mL
  • Carbonic Anhydrase IX (CA9): 20.6 - 15,000 pg/mL
  • EpCAM: 13.7 - 10,000 pg/mL
  • Kallekrein-6: 27.4-20,000 pg/mL
  • Midkine: 13.7 - 10,000 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
HCCB4MAG-58K 04054839443213

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Categorías

Life Science Research > Antibodies and Assays > Immunoassays > Multiplexed Immunoassays > Milliplex MAP Assay Products > Configurable Kits
Life Science Research > Protein Detection and Quantification > Immunoassays > Multiplexed Immunoassays > Milliplex MAP Assay Products > Configurable Kits