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Human Mesenchymal Stem Cell Media

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Serum Alternatives for Adult Stem Cell Expansion
In this webinar, Allan Dietz, Ph.D., Scientific Director of the Human Cell Therapy Laboratory (HCTL) at the Mayo Clinic in Rochester, Minnesota will describe considerations for the effective use of PLTMax® in planar and bioreactor-based expansion, and the potential for how this unique reagent will change approaches to cell culture.

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Human Mesenchymal-XF Expansion Medium

The Human Mesenchymal-XF Expansion Medium is a xeno-free medium specially formulated for the rapid expansion of human mesenchymal stem cells isolated from a variety of sources, including adipose and bone marrow tissues while maintaining multi-lineage differenitation potential.
  • Complete medium that contains all the necessary growth factors and supplements to support human mesenchymal stem cell growth.
  • All components used within the media are of human origin and do not contain any animal derived components.
  • A separate ECM solution is not required to facilitate cell attachment.
Human mesenchymal stem cell expansion media
Human adipose mesenchymal stem cell expansion using various MSC media
Improved expansion of human adipose derived MSCs (Cat. No. SCC038) over multiple passages in Human Mesenchymal-XF Expansion Medium vs. commercial and low serum competitors. 

PLTMax® Human Platelet Lysate

A Superior Alternative to FBS for Human MSC Cultures

Merck:/Freestyle/BI-Bioscience/Cell-Culture/stem-cell-images/pltmax.jpgWith the increasing interest and utilization of human cellular therapy, PLTMax® Human Platelet Lysate meets the need for a research grade cell culture supplement that is human derived, serum-free, and cost effective for human mesenchymal stem cell cultures.

Features and Benefits of PLTMax:
  • Increased cell growth kinetics vs. FBS supplemented media
  • Cost effective alternative to serum free media
  • Manufactured to reduce lot-to-lot variation
  • Stable supply and price
  • Human derived material applicable for translational research applications
Human Platelet Lysate, Cumulative Population Doubling
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Human Platelet Lysate, Total Cell Count
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PLTMax supports better growth of hMSCs than DMEM + FBS. A: Growth of hMSCs cultured in αMEM supplemented with 5 % HPL or 10 % FBS as compared to DMEM + 10 % FBS over 4 passages in T150 flasks, or B: in duplicate 125 mL spinner flasks f or 7 days, demonstrating enhanced growth in αMEM + HPL

Mesenchymal Stem Cell Freezing Media

Reliably Freeze Your Valuable Mesenchymal Stem Cell Cultures with our Validated Mesenchymal Stem Cell Freezing Media.

Human Mesenchymal Stem Cell Differentiation Media

Osteogenesis Differentiation Media

Osteogeneisis is the process of laying down new bone material by cells called osteoblasts. It is synonymous with bone tissue formation and is often studied for disease such as osteoporosis, arthritis and bone fracture regeneration.
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Mesenchymal Stem Cell Media - OsteoMAX-XF™ Differentiation Medium
Differentiation of multiple human mesenchymal stem cell lines using OsteoMAX-XF™ Differentiation Medium (EMD Millipore Cat. No. SCM121); (ii) Supplier LT Osteogenic Differentiation Kit. Differentiation was induced over 21 days in 48-well plate cultures of four different human MSC cell lines (Promocell and Life Technologies). Alizarin Red staining of representative wells at day 7, 14 and 21 are shown.
Merck’s OsteoMAX-XF™ Differentiation Medium is specially formulated to readily differentiate human mesenchymal stem cells into the osteogenic lineage as assessed by alizarin red and alkaline phosphatase staining. This proprietary formulation was developed using Plasticell’s CombiCult™, a patented, bead-based, combinatorial technology specially developed for discovery of novel stem cell differentiation protocols.

Advantages of OsteoMAX-XF™:
  • Serum-free, xeno-free, defined medium formulation
  • Rapid, robust, and highly efficient mineralization and bone nodule formation in standard tissue culture system by as early as 7 days of differentiation.
  • Proven to work in 3D BioPrinting applications
“OsteoMAX-XF produces tissue of impressive osteogenic potential, including key bone-specific and mineralization markers. We found we could rapidly fabricate functional, fully human tissue derived from MSCs and the ECM proteins they produce using the NovoGen MMX 3D-Bioprinter”
Dr. Eric Michael David MD JD, Chief Strategy Officer of Organovo

Adipogenesis Differentiation Media

Adipogenesis is the process of cell differentiation by which cells become adipocytes of fat producing cells. Adipogenesis has been one of the most intensively studied model of cellular differentiation and is often studied for energy metabolism, diabetes and obesity research.

Mesenchymal Stem Cell Adipogenesis Kit - Merck's Mesenchymal Stem Cell Adipogenesis Kit contains reagents that readily differentiate mesenchymal stem cells to an adipogenic lineage (dexamethasone, IBMX, insulin and indomethacin) as assessed with Oil Red O staining of lipid vacuoles in mature adipocytes. Along with Oil Red O staining solution, a hematoxylin solution is provided to counterstain the cell nucleus.