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ECM102 Millicoat™ Human Vitronectin Coated Strips (96-Wells)

Millicoat Cell Adhesion Strips are provided as 12 x 8-well removable strips in a 96-well plate frame for convenience & flexibility in designing assays.

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Key Applications: Activity Assay
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ECM102
1 plate  96 wells
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      Replacement Information

      Tabela com principais espec.

      Key ApplicationsDetection Methods
      ACTChromogenic
      Description
      Catalogue NumberECM102
      Brand Family Chemicon®
      Trade Name
      • Millicoat
      • Chemicon
      DescriptionMillicoat™ Human Vitronectin Coated Strips (96-Wells)
      OverviewCell adhesion plays a major role in cellular communication and regulation, and is of fundamental importance in the development and maintenance of tissues. Scientists are continually examining the adhesion and migration of many diverse cell types on various extracellular matrix (ECM) component proteins. Millicoat™ Cell Adhesion Strips are provided as 12 x 8-well removable strips in a plate frame for convenience and flexibility in designing assays. The wells in rows A - G have been coated with Human Vitronectin. Row H of each strip is coated with BSA which serves as a negative assay control. Cells are seeded onto the coated substrate. Subsequently, adherent cells are fixed and stained. Relative attachment is determined using absorbance readings.
      Alternate Names
      • Formerly under the CytoMatrixTM brand name.
      References
      Product Information
      Detection methodChromogenic
      Quality LevelMQ100
      Applications
      ApplicationMillicoat Cell Adhesion Strips are provided as 12 x 8-well removable strips in a 96-well plate frame for convenience & flexibility in designing assays.
      Key Applications
      • Activity Assay
      Application NotesPROCEDURE:

      NOTE: Optimal assay timing and performance may vary for different cell lines but generally can be obtained using subconfluent cell cultures in the assay described below. Subconfluent cultures can be achieved by splitting cells 1 to 2 days prior to performing the assay.

      1. Rehydrate the strips with 200 mL of PBS per well for at least 15 minutes at room temperature. Remove the PBS from the rehydrated strips.

      2. Prepare a single cell suspension, preferably using a non-enzymatic dissociation buffer. Optimum cell density may be determined by titration of the cells. A common starting range is between 1x10E05 to 1x10E07 cells/mL.

      3. Add 100 mL of the diluted cell suspension to each well. Incubate the plate at 37°C for 45 minutes in a CO2 incubator. Gently wash the plate 2-3 times with PBS containing Ca2+/Mg2+ (200 mL/well).

      4. Add 100 mL of 0.2% crystal violet in 10% ethanol to each well. Incubate for 5 minutes at room temperature. Remove the stain from the wells. Gently wash the strips 3-5 times with PBS (300 mL/well) to remove the excess stain.

      5. Add 100 mL of Solubilization Buffer (A 50/50 mixture of 0.1M NaH2PO4, pH 4.5 and 50% ethanol) to each well. Allow strips to incubate and gently shake at room temperature until the cell-bound stain is completely solubilized; approximately 5 minutes.

      6. Determine the absorbance at 540 - 570 nm on a microplate reader.
      Biological Information
      Species Reactivity
      • Human
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the pexin family. It is found in serum and tissues and promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. It is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond.
      Gene Symbol
      • VTN
      • VNT
      • S-protein
      • VN
      • epibolin
      • V75
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P04004 # Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.
      SIZE: 478 amino acids; 54306 Da
      SUBUNIT: Exists in two forms: a single chain 75 kDa form (V75) and a clipped form composed of two chains (65 kDa and 10 kDa) (V65+V10) which are held together by a disulfide bond. Interacts with SERPINE1/PAI1 and insulin.
      SUBCELLULAR LOCATION: Secreted, extracellular space.
      TISSUE SPECIFICITY: Plasma.
      DOMAIN: SwissProt: P04004 The SMB domain mediates interaction with SERPINE1/PAI1. The heparin-binding domain mediates interaction with insulin.
      PTM: Sulfated on 2 tyrosine residues. & N- and O-glycosylated (By similarity). & Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading. & It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.SIMILARITY: Contains 4 hemopexin-like domains. & Contains 1 SMB (somatomedin-B) domain.
      Stem Cell Type
      • Human Embryonic Stem Cells
      • Mouse Embryonic Stem Cells
      • Mesenchymal Stem Cells
      • Neural Stem Cells
      • Hematopoietic Stem Cells
      • Epithelial Cells
      • Pancreatic Stem Cells
      • Induced Pluripotent Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStrips may be stored at 2-8°C in the foil pouch for at least 3 months. Unused strips may be placed back in the pouch for storage. Ensure that the desiccant remains in the pouch, and that the pouch is securely closed.
      Packaging Information
      Material Size1 plate
      Material Package96 wells
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de catálogo GTIN
      ECM102 04053252402128