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N6639

Chlorure de bleu de nitrotétrazolium

Name: Chlorure de bleu de nitrotétrazolium
Brand: SIAL

powder, electrophoresis grade

Synonyme(s) :

Chlorure de p-nitro-bleu de tétrazolium, p-bleu de nitrotétrazolium, Chlorure de 2,2′-bis(4-nitrophényl)-5,5′-diphényl-3,3′-(3,3′-diméthoxy-4,4′-diphénylène)ditétrazolium, Chlorure de 3,3′-(3,3′-diméthoxy-4,4′-biphénylène)bis[2-(4-nitrophényl)-5-phényl-2H-tétrazolium], NBT, Nitro BT

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A propos de cet article

Formule linéaire :

C₄₀H₃₀N₁₀O₆ · 2Cl

Numéro CAS:

298-83-9

Poids moléculaire :

817.64

UNSPSC Code:

12171500

NACRES:

NA.52

PubChem Substance ID:

24897786

EC Number:

206-067-4

Beilstein/REAXYS Number:

4115923

MDL number:

MFCD00012159

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Propriétés

grade

electrophoresis grade

Quality Segment

100

assay

>90%

form

powder

technique(s)

electrophoresis: suitable

solubility

H₂O: 10 mg/mL (A stock solution at 10mg/ml in water is stable for 1-2 weeks in the dark at 0-4°C.), 2-methoxyethanol: 20 mg/mL, ethanol: 5 mg/mL

storage temp.

2-8°C

SMILES string

[Cl-].[Cl-].COc1cc(ccc1-[n+]2nc(nn2-c3ccc(cc3)[N+]([O-])=O)-c4ccccc4)-c5ccc(c(OC)c5)-[n+]6nc(nn6-c7ccc(cc7)[N+]([O-])=O)-c8ccccc8

InChI

1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3;2*1H/q+2;;/p-2

InChI key

FSVCQIDHPKZJSO-UHFFFAOYSA-L

Description

General description

Nitrotetrazolium Blue chloride (NBT) is a dye that is used for detection of alkaline phosphatase in combination with 5-bromo-4-chloro-3-indoxyl phosphate (BCIP).

Application

Nitrotetrazolium Blue chloride has been used:

to determine the superoxide anion in skeletal muscle cells
as a component in Tris-HCl buffered saline for nicotinamide adenine dinucleotide phosphate (NADPH)-diaphorase histochemistry of brain sections
as a chromogenic substrate to study the histochemical detection of hydrogen peroxide (H2O2) and superoxide (O₂- ) anion in Brassica juncea seedlings
as a component in Triton X-100 solution to perform staining of coronal brain sections

Biochem/physiol Actions

Nitrotetrazolium Blue chloride (NBT) has also been used as a colorimetric indicator of bacterial infection in blood samples. NBT has also been used as a redox indicator for other enzymatic reactions. NBT produces a dark blue insoluble formazan compound while reacting with O₂.