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Merck

87748

3,3′,5,5′-Tetramethylbenzidine

TMB substrate, chromogenic, ≥98.0% (NT), powder

Synonyme(s) :

BM blue, Sure Blue TMB, TMB, TMB Blotting Plus, TMB substrate

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About This Item

Formule linéaire :
[-C6H2(CH3)2-4-NH2]2
Numéro CAS:
54827-17-7
Poids moléculaire :
240.34
NACRES:
NA.83
PubChem Substance ID:
329769606
UNSPSC Code:
12352204
EC Number:
259-364-6
MDL number:
MFCD00007748
Beilstein/REAXYS Number:
2808541

Nom du produit

3,3′,5,5′-Tetramethylbenzidine, ≥98.0% (NT)

SMILES string

Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2

InChI key

UAIUNKRWKOVEES-UHFFFAOYSA-N

InChI

1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3

assay

≥98.0% (NT)

form

powder

technique(s)

UV/Vis spectroscopy: suitable

mp

166-171 °C
168-171 °C (lit.)

storage temp.

2-8°C

Quality Level

300

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Catégories apparentées

Application

3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases.
3,3′,5,5′-Tetramethylbenzidine has been used as a substrate for peroxidase in:
  • enzyme-immunoassay (EIA)
  • indirect competitive enzyme-linked immunosorbent assay (ELISA)
  • sandwich enzyme-linked immunosorbent assay

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Warning

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 4 - Carc. 2

Classe de stockage

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Certificats d'analyse (COA)

Lot/Batch Number
BCCH3716
BCCF7622
BCCK5557
BCCG6414
BCCD4813

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Noble DWAA, et al.
Biological journal of the Linnean Society, 111(4), 834-849 (2014)
Sally L Sherwen et al.
Zoo biology, 34(4), 287-295 (2015-06-04)
Free range exhibits are becoming increasingly popular in zoos as a means to enhance interaction between visitors and animals. However very little research exists on the impacts of visitors on animal behaviour and stress in free range exhibits. We investigated
Simon Z He et al.
Leukemia & lymphoma, 56(5), 1406-1415 (2014-09-25)
Acute myeloid leukemia (AML) blasts express high levels of interlekin-3 (IL-3) receptor-α (CD123). CSL360 is a recombinant, chimeric immunoglobulin G1 (IgG1), anti-CD123 monoclonal antibody (MoAb) that neutralizes IL-3 and demonstrates anti-leukemic activity in vitro. This phase 1 study assessed safety
Ronit Mazor et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(23), 8571-8576 (2014-05-07)
Nonhuman proteins have valuable therapeutic properties, but their efficacy is limited by neutralizing antibodies. Recombinant immunotoxins (RITs) are potent anticancer agents that have produced many complete remissions in leukemia, but immunogenicity limits the number of doses that can be given
Y Jung et al.
Mucosal immunology, 8(4), 930-942 (2015-01-08)
Eosinophils are multifunctional leukocytes that reside in the gastrointestinal (GI) lamina propria, where their basal function remains largely unexplored. In this study, by examining mice with a selective deficiency of systemic eosinophils (by lineage ablation) or GI eosinophils (eotaxin-1/2 double
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