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Merck

516560

Pertussis toxin

from Bordetella pertussis, Five distinct bands by SDS-PAGE, lyophilized, protein endotoxin, Calbiochem

Synonyme(s) :

Pertussis Toxin, Bordetella pertussis

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A propos de cet article

Numéro CAS:
70323-44-3
UNSPSC Code:
12352200
NACRES:
NA.77
MDL number:
MFCD00131841
Form:
lyophilized
Quality level:
100
Storage condition:
do not freeze

Principaux documents

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Nom du produit

Pertussis Toxin, Bordetella pertussis, This Pertussis toxin, CAS 70323-44-3, catalyzes ADP-ribosylation of guanine nucleotide-binding regulatory proteins Gi, Go, and Gt.

SMILES string

O(C)C(=O)CCCCCCCCCCCCCCCCCCCCCCCC

InChI

1S/C26H52O2/c1-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-18-19-20-21-22-23-24-25-26(27)28-2/h3-25H2,1-2H3

InChI key

WOPKHAQDUMDJIY-UHFFFAOYSA-N

description

RTECS - XW5883750

form

lyophilized

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

solubility

soluble (Reconstitute with 500 µl H2O. Obtain a uniform suspension before each use by scraping the walls of the tube and gently pipetting the solution to mix.)

foreign activity

adenylate cyclase ≤35 pmol/min-μg (in the presence of calmodulin)

shipped in

ambient

storage temp.

2-8°C

Quality Level

100

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Catégories apparentées

Analysis Note

Five distinct bands by SDS-PAGE

Application

Pertussis Toxin, Bordetella pertussis has been used as a Gi protein inhibitor to study its ability to reduce the depolarizing responses of hypothalamic pro-opiomelanocortin (POMC) neurons. It has also been used in pertussis toxin experiments to block Gαi for an in vivo inner ear and an in vitro outer hair cell (HC) study.

Biochem/physiol Actions

Primary Target
Guanine nucleotide-binding regulatory proteins Gi, Go, and Gt
Product does not compete with ATP.

Disclaimer

Toxicity: Toxic (F)

General description

Pertussis toxin (PT), secreted protein exotoxin and virulence factor by Bordetella pertussis, is the causative agent of whooping cough. It is transported across the bacterial outer membrane through a type IV secretion system encoded by the ptl genes, situated downstream from the ptx genes that encode the toxin. This complex multisubunit toxin with an AB5 configuration, serves as a crucial component of acellular pertussis vaccines. It can function protectively as a single-component toxoid vaccine. Moreover, it is widely used in mammalian cell signaling studies due to its inhibitory effect on G-protein-coupled receptor pathways. It inhibits neutrophil recruitment and airway influx, a common inflammatory response to bacterial infection.

Other Notes

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Joshi, S.A., et al. 1998. FEBS Lett. 441, 67.
Fryer, M.W. 1992. Neurosci. Lett.146, 84.
Gierschik, P. 1992. Curr. Topics Microbiol. Immunol.175, 69.
Harada, J., et al. 1992. Eur. J. Pharmacol.227, 301.
Hausman, S.Z., and Burns, D.L. 1992. J. Biol. Chem.267, 13735.
Kaslow, H.R., and Burns, D.L., 1992. FASEB J.6, 2684.
Zhu, J., et al. 1992. J. Pharmacol. Exp. Therap.263, 1479.
Hewlett, E.L., et al. 1983. Infect. Immun.40, 1198.
Moss, J., et al. 1983. J. Biol. Chem.258, 11879.

Physical form

Lyophilized from 50 mM NaCl, 10 mM sodium phosphate buffer, pH 7.0.

Preparation Note

Following reconstitution, refrigerate (4°C). For long-term storage in solution, reconstitute with a high ionic strength buffer, such as 100 mM sodium phosphate, 500 mM sodium chloride, pH 7.0. Stock solutions are stable for at least 6 months for use in CHO cell assays and for 1-2 months for use in non-cellular systems.
Reconstitute with 500 µl H₂O. Obtain a uniform suspension before each use by scraping the walls of the tube and gently pipetting the solution to mix.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Classe de stockage

13 - Non Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Pertussis Toxin: Structure-Function Relationship
Locht C, et al.
Bacterial Protein Toxins, 167-185 (2000)
Nicholas H Carbonetti
Future microbiology, 5(3), 455-469 (2010-03-10)
Pertussis toxin and adenylate cyclase toxin are two important virulence factors of Bordetella pertussis, the bacterial cause of the respiratory disease pertussis or whooping cough. In addition to studies on the structure, function and role in pathogenesis of these two
Live imaging of hair bundle polarity acquisition demonstrates a critical timeline for transcription factor Emx2
Tona Y and Wu DK
eLife, 9, e59282-e59282 (2020)
P Órdenes et al.
Scientific reports, 11(1), 21644-21644 (2021-11-06)
Previous studies indicate that the activity of hypothalamic POMC neurons can be regulated by glucose via intracellular mechanisms, but its regulation by lactate is poorly understood. In addition to its energetic role, lactate acts as a signaling molecule. In this

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