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Merck

A2554

Anti-Mouse IgG (Fc specific)–Peroxidase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

Synonyme(s) :

Goat Anti-Mouse IgG (Fc specific)–HRP

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00162629

Nom du produit

Anti-Mouse IgG (Fc specific)–Peroxidase antibody produced in goat, affinity isolated antibody, buffered aqueous solution

biological source

goat

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

mouse

should not react with

bovine, human, horse

technique(s)

direct ELISA: 1:70,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200
western blot (chemiluminescent): 1:80,000-1:160,000 using total cell extract of Hela cells (5-10 μg/mL)

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

300

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Catégories apparentées

Application

Anti-Mouse IgG (Fc specific)-Peroxidase antibody produced in goat has been used in:
  • immunohistochemistry
  • enzyme-linked immunosorbent assay (ELISA)
  • immunoblot
  • dot-blot analysis

Specificity of a new antibody for PND in blood from immunized mice was tested by Elisa using biotynlated PND and streptavidin coated plates. HRP conjugated goat anti-mouse IgG (Fc specific) was used as secondary.

Biochem/physiol Actions

Goat anti-mouse IgG(Fc specific)-peroxidase antibody is specific for mouse IgG and mouse IgG, Fc fragment. The conjugate shows no reactivity with mouse Fab fragment, human IgG, IgA, IgM, κ and λ light chain, bovine IgG and IgM, or horse IgG.
Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice.
Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. IgG helps in opsonization and antibody dependent cell mediated cytotoxicity.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. Each immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein. IgG is a major class of immunoglobulin. Mice consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
Antibody adsorbed with bovine, equine and human serum proteins.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT.

Preparation Note

Adsorbed to reduce background staining with bovine, horse, or human samples.
Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

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pictograms

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GHS07

signalword

Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1

Classe de stockage

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificats d'analyse (COA)

Lot/Batch Number
0000466581
0000466581
0000452733
0000452733
0000225296

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Consulter la Bibliothèque de documents

Ryan V Petty et al.
Journal of virology, 88(22), 13503-13509 (2014-09-12)
The leader (L) and 2A proteins of cardioviruses are the primary antihost agents produced during infection. For encephalomyocarditis virus (EMCV), the prototype of the genus Cardiovirus, these proteins interact independently with key cellular partners to bring about inhibition of active
Design and use of a phage display library Human antibodies with subnanomolar affinity against a marker of angiogenesis eluted from a two-dimensional gel
Pini A, et al.
The Journal of Biological Chemistry, 273(34), 21769-21776 (1998)
Adenovirus-mediated soluble FLT-1 gene therapy for ovarian carcinoma
Mahasreshti PJ, et al.
Clinical Cancer Research, 7(7), 2057-2066 (2001)
TGF-beta neutralization enhances AngII-induced aortic rupture and aneurysm in both thoracic and abdominal regions
Chen X, et al.
Testing, 11(4), e0153811-e0153811 (2016)
Christoph Bock et al.
Scientific reports, 9(1), 11884-11884 (2019-08-17)
The lysosomal polypeptide transporter TAPL belongs to the superfamily of ATP-binding cassette transporters. TAPL forms a homodimeric transport complex, which translocates oligo- and polypeptides into the lumen of lysosomes driven by ATP hydrolysis. Although the structure and the function of
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