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Merck

A4789

Anti-Mouse IgA (α-chain specific)−Peroxidase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

Synonyme(s) :

Goat Anti-Mouse IgA (α-chain specific)−HRP

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A propos de cet article

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
Conjugate:
peroxidase conjugate
Clone:
polyclonal
Application:
ELISA (d)
Citations:
28
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biological source

goat

Quality Level

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

direct ELISA: 1:10,000

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

IgA antibody plays an essential role in mucosal immunity and binds with immunogens/pathogens to restrict them from entering the mucosal membrane. Anti-mouse IgA (α-chain specific)-peroxidase antibody can be used for determining the secretory IgA (sIgA) in intestinal fluid. It can also be used in direct ELISA. Goat anti-mouse IgA (α-chain specific)-peroxidase antibody reacts specifically with mouse IgA.

Immunogen

Purified mouse IgA

Application

Anti-mouse IgA (α-chain specific)-peroxidase antibody can be used in modified indirect DIBA (dot- immunobinding assay) .
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Enzyme-linked immunosorbent assay (1 paper)
Western Blotting (1 paper)
ELISAS were performed using HRP conjugated gaot anti-mouse IgA as the secondary antibody at a dilution of 1:10000 for a 1 hour incubation at 37 degrees to detect IgA levels in mouse fecal extracts.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin with preservative.

Preparation Note

Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p 215 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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hcodes

Hazard Classifications

Aquatic Chronic 3 - Skin Sens. 1

Classe de stockage

12 - Non Combustible Liquids

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WGK 2


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Consulter la Bibliothèque de documents

A C Rodrigues et al.
Journal of applied microbiology, 89(3), 404-414 (2000-10-06)
The effect of Saccharomyces boulardii on the immune system was evaluated, comparing germ-free Swiss/NIH mice monoassociated with the probiotic with germ-free mice. Saccharomyces boulardii colonized the gut of germ-free mice and survived the gastrointestinal conditions. An increase in sIgA production
The Immune Response in Mice Immunized with Lactobacillus acidophilus LF221 - A Potential Probiotic Strain.
Irena Rogelj
Food Technology and Biotechnology, 37(3), 153-158 (1999)
J M Ball et al.
Journal of virology, 72(2), 1345-1353 (1998-01-28)
Recombinant Norwalk virus-like particles (rNV VLPs) produced in insect cells were evaluated as an oral immunogen in CD1 and BALB/c mice by monitoring rNV-specific serum total and subclass immunoglobulin G (IgG) and intestinal IgA responses. Dose and kinetics of response
Catalina Lopez-Saucedo et al.
BioMed research international, 2015, 679850-679850 (2015-06-13)
Individuals with X-HIGM syndrome fail to express functional CD40 ligand; consequently they cannot mount effective protective antibody responses against pathogenic bacteria. We evaluated, compared, and characterized the humoral immune response of wild type (WT) and C57-CD40L deficient (C57-CD40L(-/-)) mice infected
Sidonia Fagarasan et al.
Nature reviews. Immunology, 3(1), 63-72 (2003-01-04)
Immunoglobulin A is the most abundant immunoglobulin isotype in mucosal secretions. In this review, we summarize recent advances in our understanding of the sites, mechanisms and functions of intestinal IgA synthesis in mice. On the basis of these recent findings

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