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CELLMM2

FLAG® M Purification Kit

For Mammalian expression systems.

Synonyme(s) :

Anti-ddddk, Anti-dykddddk

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NACRES:
NA.32
UNSPSC Code:
12352200
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Quality Level

200

technique(s)

protein extraction: suitable

shipped in

wet ice

storage temp.

−20°C

Application

The FLAG Purification Kit utilizes CelLytic™ Reagents for rapid and efficient cell lysis and protein extraction and ANTI-FLAG® M2 affinity gel for affinity purification of active FLAG fusion proteins.

Learn more product details in our FLAG® application portal.

Features and Benefits

  • Includes ready to use reagents, columns, and a detailed protocol for affinity purification of FLAG fusion proteins.
  • ANTI-FLAG M2 Affinity Gel allows efficient binding of FLAG fusion proteins without the need for preliminary steps and calibrations.
  • Two alternatives for efficient elution conditions (by acidic conditions or by competition with FLAG peptide).

Packaging

Sufficient for 3-5 uses of 1-ml affinity purification column.

Other Notes

Kit contains CelLytic™ M for cell lysis and protein extraction from Mammalian cells.

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
CelLytic is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Composants de kit également disponibles séparément

Réf. du produit
Description
FDS & Tarif
  • C2978
    CelLytic™ M, Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization.
    FDS
  • SAE0194
    Purified 3xFLAG® peptide, ≥95% (HPLC), lyophilized powder
    FDS
  • A2220
    ANTI-FLAG® M2 Affinity Gel, purified immunoglobulin, buffered aqueous glycerol solution
    FDS
  • C2103
    Chromatography columns, general-purpose, volume 10 mL, Overall H 5 in.
    FDS

Classe de stockage

10 - Combustible liquids

wgk

WGK 3

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Certificats d'analyse (COA)

Lot/Batch Number
0000524039
0000524039
0000240927
0000240927
0000176020

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Consulter la Bibliothèque de documents

Takeshi Into et al.
Molecular and cellular biology, 28(4), 1338-1347 (2007-12-19)
Nitric oxide (NO) has been thought to regulate the immune system through S nitrosylation of the transcriptional factor NF-kappaB. However, regulatory effects of NO on innate immune responses are unclear. Here, we report that NO has a capability to control
M D Mostaqul Huq et al.
Molecular & cellular proteomics : MCP, 6(4), 677-688 (2007-01-09)
Retinoic acid receptors (RARs) belong to the nuclear receptor superfamily. The mechanism of ligand-dependent activation of RARs is well known. The effect of protein phosphorylation on the activity of RARs has also been demonstrated. However, it is unclear whether other
Severa Bunda et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(36), E3785-E3794 (2014-08-27)
Mutations in Ras GTPase and various other components of the Ras signaling pathways are among the most common genetic alterations in human cancers and also have been identified in several familial developmental syndromes. Over the past few decades it has
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