D5546
DMEM - low glucose
With sodium bicarbonate and sodium pyruvate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture
Synonyme(s) :
DME, Dulbecco′s Modified Eagle′s Medium - high glucose, DMEM
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Dulbecco′s Modified Eagle′s Medium - low glucose, With 1000 mg/L glucose, and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
NaHCO3: yes
phenol red: yes
glucose: low
HEPES: no
sodium pyruvate: yes
L-glutamine: no
shipped in
ambient
storage temp.
2-8°C
General description
Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains a higher concentration of amino acids, vitamins, and additional supplementary components. The original DMEM formula, first reported for culturing embryonic mouse cells, contained 1,000 mg/L of glucose. An alteration with 4,500 mg/L glucose is optimal in cultivating certain cell types. This modification has been shown to improve the stability of the medium.
Application
Dulbecco′s Modified Eagle′s Medium - low glucose has been used to culture:
- human osteosarcoma cells and HeLa cells
- primary human mesenchymal stromal cells (hBMSCs)
- human embryonic kidney cell lines
Preparation Note
Supplement with 0.584 g/L glutamine.
Classe de stockage
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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