F2012
Anti-Mouse IgG (whole molecule)–FITC antibody produced in goat
affinity isolated antibody, buffered aqueous solution
Synonyme(s) :
Goat Anti-Mouse IgG (whole molecule)–Fluorescein isothiocyanate
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A propos de cet article
Conjugate:
FITC conjugate
Clone:
polyclonal
Application:
direct immunofluorescence
Technique(s):
direct immunofluorescence: 1:64 (minimum)
Citations:
104
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Laissez-nous vous aiderNom du produit
Anti-Mouse IgG (whole molecule)–FITC antibody produced in goat, affinity isolated antibody, buffered aqueous solution
biological source
goat
conjugate
FITC conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
technique(s)
direct immunofluorescence: 1:64 (minimum)
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
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Application
Anti-Mouse IgG (whole molecule)−FITC antibody has been used:
- in immunofluorescence
- in immunohistochemistry
- in immunofluorescence staining
- in immunofluorescence microscopy
- in confocal imaging
Anti-Mouse IgG (whole molecule)-FITC antibody produced in goat was used for FACS analysis of DF3 antigen on breast cancer cell lines.
Biochem/physiol Actions
IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of FITC to Anti-Mouse IgG (whole molecule) antibody allows for the visualization of protein by fluorescent microscopy.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by disulfide bond. It mainly helps in immune defense. It is a glycoprotein and a major class of immunoglobulin. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.
The product binds all mouse Igs.
Other Notes
Antibody adsorbed with human serum proteins.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
Preparation Note
Adsorbed to reduce background staining with human samples
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Classe de stockage
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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Li D, et al.
Journal of virology, 414(2-3), JVI-03130 (2013)
Exposure to ELF-pulse modulated X band microwaves increases in vitro human astrocytoma cell proliferation
Perez-Castejon C, et al.
Histology and Histopathology, 24(10), 1551-1551 (2009)
Stimulation of intracellular sphingosine-1-phosphate production by G-protein-coupled sphingosine-1-phosphate receptors
zu H D M, et al.
European Journal of Pharmacology, 414(2-3), 145-154 (2001)
L Chen et al.
The Journal of clinical investigation, 96(6), 2775-2782 (1995-12-01)
The high molecular weight mucin-like glycoprotein, DF3 (MUC1), is overexpressed in the majority of human breast cancers. Here we demonstrate that replication defective recombinant adenoviral vectors, containing the DF3 promoter (bp -725 to +31), can be used to express beta-galactosidase
Adhesion and differentiation of human mesenchymal stem cells on plasma-functionalized graphenes with different feeding gases
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