N6658
Nutrient Mixture F-12 Ham
With ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Synonyme(s) :
Ham’s F-12
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Nutrient Mixture F-12 Ham, With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
L-glutamine: yes
HEPES: no
phenol red: yes
sodium pyruvate: 0.11 g/L
NaHCO3: yes
shipped in
ambient
storage temp.
2-8°C
General description
Ham′s Nutrient Mixtures were mainly created to support the clonal growth of numerous clones of Chinese hamster ovary (CHO) cells and clones of HeLa and mouse L-cells. It is also used to grow primary rat hepatocytes and rat prostate epithelial cells. Ham′s F-12 is used as the medium of choice for a clonal toxicity assay using CHO cells.
Application
Nutrient Mixture F-12 Ham has been used:
- to culture Chinese hamster ovary cells overexpressing insulin receptor (CHO-IR)
- to culture LAN-1 cells
- to maintain SUM149, SUM159, SUM190, FC-IBC02, BCX010, and IBC3 cells
- with Dulbecco’s modified Eagle’s medium (DMEM) media in a 1:1 ratio to culture oral cavity squamous cell carcinoma (OSCC) cell lines.
Classe de stockage
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Contenu apparenté
Yoshinobu Takahashi et al.
Oncology letters, 14(1), 264-270 (2017-07-12)
Whether the poor prognosis of primary central nervous system lymphoma (PCNSL) compared with systemic diffuse large B cell lymphoma (DLBCL) is attributable to the immune privilege of the intracerebral location or to intrinsic differences in the biological characteristics of two
Ali I Mohammed et al.
Biomolecules, 13(8) (2023-08-26)
Reactive oxygen species (ROS) are highly reactive molecules generated in living organisms and an excessive production of ROS culminates in oxidative stress and cellular damage. Notably, oxidative stress plays a critical role in the pathogenesis of a number of oral
Jianjia Fan et al.
Journal of lipid research, 59(5), 830-842 (2018-03-23)
apoE is the primary lipid carrier within the CNS and the strongest genetic risk factor for late onset Alzheimer's disease (AD). apoE is primarily lipidated via ABCA1, and both are under transcriptional regulation by the nuclear liver X receptor (LXR).