RIP
Imprint® RNA Immunoprecipitation Kit
High-capacity Protein A magnetic beads for successful RNA Immunoprecipitation,suitable for use with mRNA and microRNA
Synonyme(s) :
Magnetic Bead RNA Immunoprecipitation, RNA Immunopreciptation, mRNA Immunopreciptation, microRNA Immunoprecipitation
Se connecter pour consulter les tarifs organisationnels et contractuels.
Sélectionner une taille de conditionnement
A propos de cet article
NACRES:
NA.54
UNSPSC Code:
41116158
Service technique
Besoin d'aide ? Notre équipe de scientifiques expérimentés est là pour vous.
Laissez-nous vous aiderService technique
Besoin d'aide ? Notre équipe de scientifiques expérimentés est là pour vous.
Laissez-nous vous aiderQuality Level
Catégories apparentées
General description
Our Imprint RNA Immunoprecipitation Kit provides all reagents needed for successful RIP when paired with an appropriate antibody. The kit includes two lysis buffers, one mild for less tightly bound RNPs, the other harsh for strongly associated RNPs. Protein A magnetic beads are provided to collect immune precipitates and facilitate washing. The kit also includes both rabbit and mouse IgG for negative control RIPs, a rabbit anti-mouse bridging antibody to bind mouse monoclonal primary antibodies efficiently to protein A on the magnetic beads, as well as both protease and ribonuclease inhibitors to protect the RNPs from degradation during RIP.
Learn more about this product and view application data.
Learn more about this product and view application data.
RNA immunoprecipitation (RIP) is a very powerful procedure for the study of RNA binding proteins (RBPs) and their RNA targets in ribonucleoprotein (RNP) complexes. Antibodies raised against specific RBPs are used to coprecipitate RNPs, i.e., the RBP along with its RNA partner. The RNA can then be identified by next generation sequencing, or if testing for a specific RNA, by RT-PCR.
Application
Imprint® RNA Immunoprecipitation Kit has been used for:
- Suitable for downstream applications
- Individual target characterization to genome-wide profiling techniques
- Characterization of signal transduction pathways
- Verification of ChIp-chIP and ChIP-seq data
Features and Benefits
- Xtra Protein A magnetic beads with higher binding capacity
- Step-by-step protocols for cell lysis and immunoprecipitation
- RNAse inhibitors and RNAse-free reagents to maintain RNA integrity
- Negative control and antibody included
Legal Information
Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany
Composants de kit également disponibles séparément
Réf. du produit
Description
FDS
- I5381IgG from mouse serum, reagent grade, ≥95% (SDS-PAGE), lyophilized powder
- I5006IgG from rabbit serum, reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder
- M7023Anti-Mouse IgG (whole molecule) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
- P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution
- R1158Ribonuclease Inhibitor, recombinant, expressed in E. coli, 20-40 units/μL
Classe de stockage
3 - Flammable liquids
wgk
WGK 3
flash_point_f
100.4 °F
flash_point_c
38 °C
signalword
Danger
Hazard Classifications
Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2 - Flam. Liq. 3 - Skin Irrit. 2
Faites votre choix parmi les versions les plus récentes :
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Yiwei Liao et al.
Journal of cellular biochemistry, 118(7), 1889-1899 (2017-01-26)
Temozolomide (TMZ)-based chemotherapy is a standard strategy for glioma, while chemoresistance remains a major therapeutic challenge. Recent evidence highlights the crucial regulatory roles of long non-coding RNAs (lncRNA) in tumor biology. However, the roles and regulatory mechanisms of lncRNA cancer
Qiyuan Wang et al.
Journal of cellular biochemistry, 118(11), 3775-3784 (2017-04-06)
Osteoarthritis (OA) is characterized by progressive destruction of articular cartilage, resulting in significant disability. Inflammatory cytokines commonly initiate the extreme changes in the synovium and cartilage microenvironment of the OA patients, subsequently resulting in cell dysfunctions, especially synoviocyte dysfunction. We
Shuai Xiao et al.
DNA and cell biology, 37(2), 117-125 (2018-02-08)
P73 antisense RNA 1T (non-protein coding), known as TP73-AS1 or PDAM, is a long noncoding RNA (lncRNA), which may regulate apoptosis by regulation of p53-dependent antiapoptotic genes. An abnormal change of TP73-AS1 expression was noticed in cancers. The effects of
Nicola Amodio et al.
Leukemia (2018-03-01)
The biological role and therapeutic potential of long non-coding RNAs (lncRNAs) in multiple myeloma (MM) are still to be investigated. Here, we studied the functional significance and the druggability of the oncogenic lncRNA MALAT1 in MM. Targeting MALAT1 by novel
Lin Cao et al.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 93, 570-579 (2017-07-08)
Non-small cell lung cancer (NSCLC), known as the most common type of lung cancer, has caused great economic losses and brought the patients with NSCLC great suffering. The NSCLC chemo-resistance to cisplatin (DDP) -based chemotherapy remains a huge challenge. The
Contenu apparenté
Instructions
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| 03-902 | 04065265138673 |
| 03-901 | 04065265138680 |
| 03-176 | 04053252291005 |
| 03-191 | 04053252518928 |
| 03-110 | 04053252679209 |
| 03-111 | 04053252679216 |
| 03-119 | 04053252376474 |
| 03-204 | 04053252682308 |
| MAGNARIP03 | 04053252666650 |
| 03-102 | 04053252583209 |
| 03-104 | 04053252468421 |
| 03-101 | 04053252320415 |
| 03-200 | 04053252480621 |
| 03-178 | 04053252480614 |
| 03-196 | 04053252376498 |
| 03-179 | 04053252376481 |
| 17-704 | 04053252025426 |
| 03-248 | 04053252756535 |
| 03-107 | 04053252679186 |
| 03-206 | 04053252556128 |
| 03-113 | 04053252679759 |
| 03-900 | 04065265138697 |
| 03-181 | 04053252682292 |
| 17-700 | 04053252010880 |
| 17-701 | 04053252000867 |
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique